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The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus

Expression analysis ofASL3:(A) RT–PCR analysis ofASL3in root, young stem, young leaf, flag leaf and panicle of WT. Rice Actin gene was used as a control; (B) Transcript levels of ASL3 in top leaves sampled from WT, asl3 mutant, RNAi lines at 3-leaf-stage.
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Fig4: Expression analysis ofASL3:(A) RT–PCR analysis ofASL3in root, young stem, young leaf, flag leaf and panicle of WT. Rice Actin gene was used as a control; (B) Transcript levels of ASL3 in top leaves sampled from WT, asl3 mutant, RNAi lines at 3-leaf-stage.

Mentions: To understand whether the function-loss of ASL3 is responsible for the lethal phenotype in mutant, RNA interference (RNAi) technology was used to suppress ASL3 expression in WT plants. A gene-specific fragment of ASL3 was cloned into an RNAi vector and transgenic plants were generated via Agrobacterium–mediated transformation. Resultantly, fifty-one RNAi lines showed the same albino phenotypes as in the asl3 mutant (Figure 1C). Further, two RNAi transgenic plants with albino phenotypes were selected for measurement of ASL3 transcript. The ASL3 transcripts of RNAi lines were significantly lower than that of the WT plants (Figure 4B). These results confirmed that RNAi of ASL3 could mimic the phenotypes of the asl3 mutant.Figure 4


The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Expression analysis ofASL3:(A) RT–PCR analysis ofASL3in root, young stem, young leaf, flag leaf and panicle of WT. Rice Actin gene was used as a control; (B) Transcript levels of ASL3 in top leaves sampled from WT, asl3 mutant, RNAi lines at 3-leaf-stage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390607&req=5

Fig4: Expression analysis ofASL3:(A) RT–PCR analysis ofASL3in root, young stem, young leaf, flag leaf and panicle of WT. Rice Actin gene was used as a control; (B) Transcript levels of ASL3 in top leaves sampled from WT, asl3 mutant, RNAi lines at 3-leaf-stage.
Mentions: To understand whether the function-loss of ASL3 is responsible for the lethal phenotype in mutant, RNA interference (RNAi) technology was used to suppress ASL3 expression in WT plants. A gene-specific fragment of ASL3 was cloned into an RNAi vector and transgenic plants were generated via Agrobacterium–mediated transformation. Resultantly, fifty-one RNAi lines showed the same albino phenotypes as in the asl3 mutant (Figure 1C). Further, two RNAi transgenic plants with albino phenotypes were selected for measurement of ASL3 transcript. The ASL3 transcripts of RNAi lines were significantly lower than that of the WT plants (Figure 4B). These results confirmed that RNAi of ASL3 could mimic the phenotypes of the asl3 mutant.Figure 4

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus