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The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus

Map-Based cloning ofASL3: (A) TheASL3locus was initially mapped to a region between markers RM488 and RM297 on the long arm of rice chromosome 1 (Chr.1) with 160 recessive individuals; (B) Fine mapping ofASL3between BAC1 (AP008207.2) and BAC2 (AP006867) within a 32-kb region by the markers P3 and P4 using 4,213 mutant individuals; (C) Diagram of the predicted ORFs and the mutation site; (D) Gene model ofASL3, a 1-bp deletion (G*) inLOC_Os01g48380results in a premature stop codon.
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Fig3: Map-Based cloning ofASL3: (A) TheASL3locus was initially mapped to a region between markers RM488 and RM297 on the long arm of rice chromosome 1 (Chr.1) with 160 recessive individuals; (B) Fine mapping ofASL3between BAC1 (AP008207.2) and BAC2 (AP006867) within a 32-kb region by the markers P3 and P4 using 4,213 mutant individuals; (C) Diagram of the predicted ORFs and the mutation site; (D) Gene model ofASL3, a 1-bp deletion (G*) inLOC_Os01g48380results in a premature stop codon.

Mentions: The ASL3 locus was initially mapped to the long arm of chromosome 1(Chr1) between the molecular markers RM488 and RM297 by analyzing 160 mutant individuals (Figure 3A). Then a larger F2 population with 4213 mutant individuals was used for fine mapping. Eight InDel markers (P1 → P8) were developed between RM488 and RM297. The ASL3 locus was further narrowed down to a 32-kb region between P3 and P4 (Figure 3B), which included 3 putative open reading frames (ORFs) (http://rice.plantbiology.msu.edu) (Figure 3C). All putative ORFs were sequenced and a 1-bp deletion (G*) was found in LOC_Os01g48380, causing a premature stop codon (Figure 3D).Figure 3


The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Map-Based cloning ofASL3: (A) TheASL3locus was initially mapped to a region between markers RM488 and RM297 on the long arm of rice chromosome 1 (Chr.1) with 160 recessive individuals; (B) Fine mapping ofASL3between BAC1 (AP008207.2) and BAC2 (AP006867) within a 32-kb region by the markers P3 and P4 using 4,213 mutant individuals; (C) Diagram of the predicted ORFs and the mutation site; (D) Gene model ofASL3, a 1-bp deletion (G*) inLOC_Os01g48380results in a premature stop codon.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390607&req=5

Fig3: Map-Based cloning ofASL3: (A) TheASL3locus was initially mapped to a region between markers RM488 and RM297 on the long arm of rice chromosome 1 (Chr.1) with 160 recessive individuals; (B) Fine mapping ofASL3between BAC1 (AP008207.2) and BAC2 (AP006867) within a 32-kb region by the markers P3 and P4 using 4,213 mutant individuals; (C) Diagram of the predicted ORFs and the mutation site; (D) Gene model ofASL3, a 1-bp deletion (G*) inLOC_Os01g48380results in a premature stop codon.
Mentions: The ASL3 locus was initially mapped to the long arm of chromosome 1(Chr1) between the molecular markers RM488 and RM297 by analyzing 160 mutant individuals (Figure 3A). Then a larger F2 population with 4213 mutant individuals was used for fine mapping. Eight InDel markers (P1 → P8) were developed between RM488 and RM297. The ASL3 locus was further narrowed down to a 32-kb region between P3 and P4 (Figure 3B), which included 3 putative open reading frames (ORFs) (http://rice.plantbiology.msu.edu) (Figure 3C). All putative ORFs were sequenced and a 1-bp deletion (G*) was found in LOC_Os01g48380, causing a premature stop codon (Figure 3D).Figure 3

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus