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The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy of chloroplasts in expanded third leaves: (A) The cell of wild type; (B) An intact chloroplast in the wild type cell; (C) The cell of anasl3mutant; (D) An abnormal chloroplast in theasl3mutant cell. c, chloroplast; g, grana stack.
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Fig2: Transmission electron microscopy of chloroplasts in expanded third leaves: (A) The cell of wild type; (B) An intact chloroplast in the wild type cell; (C) The cell of anasl3mutant; (D) An abnormal chloroplast in theasl3mutant cell. c, chloroplast; g, grana stack.

Mentions: To investigate chloroplast development in asl3 mutant, the ultrastructure of chloroplasts at 3-leaf stages were examined by transmission electron microscopy (TEM). As expected, the unabridged chloroplast was found in all WT plants and the grana stacks were dense and well structured (Figure 2A,B), whereas chloroplast did not display the usual architecture and had no observable grana lamella stacks in asl3 mutant (Figure 2C,D). These observations indicate that the asl3 mutation results in abnormal development of the chloroplasts.Figure 2


The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Transmission electron microscopy of chloroplasts in expanded third leaves: (A) The cell of wild type; (B) An intact chloroplast in the wild type cell; (C) The cell of anasl3mutant; (D) An abnormal chloroplast in theasl3mutant cell. c, chloroplast; g, grana stack.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390607&req=5

Fig2: Transmission electron microscopy of chloroplasts in expanded third leaves: (A) The cell of wild type; (B) An intact chloroplast in the wild type cell; (C) The cell of anasl3mutant; (D) An abnormal chloroplast in theasl3mutant cell. c, chloroplast; g, grana stack.
Mentions: To investigate chloroplast development in asl3 mutant, the ultrastructure of chloroplasts at 3-leaf stages were examined by transmission electron microscopy (TEM). As expected, the unabridged chloroplast was found in all WT plants and the grana stacks were dense and well structured (Figure 2A,B), whereas chloroplast did not display the usual architecture and had no observable grana lamella stacks in asl3 mutant (Figure 2C,D). These observations indicate that the asl3 mutation results in abnormal development of the chloroplasts.Figure 2

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus