A genetic screen and transcript profiling reveal a shared regulatory program for Drosophila linker histone H1 and chromatin remodeler CHD1.
Bottom Line: It has a profound effect on organization of chromatin into higher-order structures and on recruitment of histone-modifying enzymes to chromatin.We identify 41 mis-expression alleles that enhance and 20 that suppress the effect of His1 depletion in vivo.Specifically, the reduced viability of H1-depleted animals is strongly suppressed by ubiquitous mis-expression of the ATP-dependent chromatin remodeling enzyme CHD1.
Affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.Show MeSH
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Mentions: To confirm the effects of H1 on these genes, we performed quantitative RT-PCR to compare mRNA expression of some of the immune response–related genes in H1-depleted and control salivary glands. We discovered a consistent correspondence between the microarray and RT-PCR data: genes that were detected as repression targets of H1 by microarray analyses also exhibited upregulation in H1-depleted salivary glands when examined by RT-PCR (Figure 2A and data not shown). Many additional immune response–related genes that were not originally identified as H1 targets by microarray analyses also exhibit strong upregulation (Figure 2A). It is of interest that many of these genes are repressed by a chromatin-remodeling factor CHD1 (Sebald et al. 2012), and Chd1 was identified as one of the His1-interacting genes in our mis-expression screen (Table 2).
Affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.