A genetic screen and transcript profiling reveal a shared regulatory program for Drosophila linker histone H1 and chromatin remodeler CHD1.
Bottom Line: It has a profound effect on organization of chromatin into higher-order structures and on recruitment of histone-modifying enzymes to chromatin.We identify 41 mis-expression alleles that enhance and 20 that suppress the effect of His1 depletion in vivo.Specifically, the reduced viability of H1-depleted animals is strongly suppressed by ubiquitous mis-expression of the ATP-dependent chromatin remodeling enzyme CHD1.
Affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.Show MeSH
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Mentions: Ten virgin Act5C-GAL4, pINT-H14M/SM5 or Act5C-GAL4/SM5 females were mated with 10 males carrying an EP insertion on the second chromosome (534 alleles), either homozygous or balanced heterozygous (Figure 1, A and B), and reared at 27°. Otherwise, the crosses were performed, and the adult progeny were scored exactly as described above. At least 50 adult F1 flies were scored for each cross.
Affiliation: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.