Conserved RNA-binding proteins required for dendrite morphogenesis in Caenorhabditis elegans sensory neurons.
Bottom Line: Homologs of each of these genes were previously identified as important in the Drosophila melanogaster dendritic arborization sensory neurons.Our results suggest that RNA processing, mRNA localization, mRNA stability, and translational control are all important mechanisms that contribute to dendrite morphogenesis, and we present a conserved set of RNA-binding proteins that regulate these processes in diverse animal species.Furthermore, homologs of these genes are expressed in the human brain, suggesting that these RNA-binding proteins are candidate regulators of dendrite development in humans.
Affiliation: Department of Molecular Biology, Colorado College, Colorado Springs, Colorado 80903.Show MeSH
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Mentions: To test whether these RBPs function cell-autonomously in the PVD neuron and to determine if RBP::GFP fusion protein subcellular localization is biologically relevant, we expressed each RBP::GFP transgene specifically in the PVD neuron and tested for its ability to rescue PVD dendrite defects in RBP mutant animals (see the section Materials and Methods). We found that PVD-specific expression of CGH-1, MBL-1, and Y55F3AM.3 fusion proteins to GFP confer a statistically significant complete rescue of the PVD dendritic termini defects in the respective mutants (Figure 7). This strongly suggests that CGH-1, MBL-1, and Y55F3AM.3 each function cell-autonomously in the PVD to control dendrite development. Furthermore, the subcellular localizations inferred from these RBP::GFP fusions proteins are likely accurate because these proteins are functional.
Affiliation: Department of Molecular Biology, Colorado College, Colorado Springs, Colorado 80903.