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Conserved RNA-binding proteins required for dendrite morphogenesis in Caenorhabditis elegans sensory neurons.

Antonacci S, Forand D, Wolf M, Tyus C, Barney J, Kellogg L, Simon MA, Kerr G, Wells KL, Younes S, Mortimer NT, Olesnicky EC, Killian DJ - G3 (Bethesda) (2015)

Bottom Line: Homologs of each of these genes were previously identified as important in the Drosophila melanogaster dendritic arborization sensory neurons.Our results suggest that RNA processing, mRNA localization, mRNA stability, and translational control are all important mechanisms that contribute to dendrite morphogenesis, and we present a conserved set of RNA-binding proteins that regulate these processes in diverse animal species.Furthermore, homologs of these genes are expressed in the human brain, suggesting that these RNA-binding proteins are candidate regulators of dendrite development in humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Colorado College, Colorado Springs, Colorado 80903.

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Cytoplasmic localization of CGH-1, CPB-3, LARP-5, and SUP-26 in PVD neurons. cDNAs for RNA-binding protein (RBP) genes indicated were fused to green fluorescent protein (GFP) under the control of a PVD-specific promoter and reveal cytoplasmic localization. PVDs were marked by ser-2prom3::myr-mCherry. Arrowheads indicate GFP-positive particles within dendrites. Bar = 5 μm.
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fig6: Cytoplasmic localization of CGH-1, CPB-3, LARP-5, and SUP-26 in PVD neurons. cDNAs for RNA-binding protein (RBP) genes indicated were fused to green fluorescent protein (GFP) under the control of a PVD-specific promoter and reveal cytoplasmic localization. PVDs were marked by ser-2prom3::myr-mCherry. Arrowheads indicate GFP-positive particles within dendrites. Bar = 5 μm.

Mentions: DDX-17, MBL-1, MTR-4, RSP-3, RSP-6, SET-2, and Y55F3AM.3 translation fusions to GFP show clear nuclear expression (Figure 5). This finding is consistent with these RBPs playing roles in RNA processing within the nucleus (see the section Discussion). Conversely, we find that CGH-1, CPB-3, LARP-5, and SUP-26 translational fusions to GFP are localized to the cytoplasm (Figure 6). More specifically, CGH-1::GFP is enriched in the perinuclear region and small puncta are present throughout the cytoplasm, including particles in dendrites (Figure 6). Similarly, CPB-3::GFP also is found in puncta in the cytoplasm of the cell body and dendrites. However, CPB-3 puncta are substantially larger and lack perinuclear enrichment (Figure 6). Unlike CGH-1 and CPB-3, SUP-26::GFP is restricted to the cytoplasm of the cell body and is not found in dendrites. SUP-26 has a striking, perinuclear enrichment with intermediate-sized puncta, relative to CGH-1 and CPB-3, located further from the nucleus but still within the cell body (Figure 6). Finally, LARP-5::GFP is diffuse throughout the cytoplasm, including the dendrites, and the nucleus and does not localize to discrete puncta (Figure 6).


Conserved RNA-binding proteins required for dendrite morphogenesis in Caenorhabditis elegans sensory neurons.

Antonacci S, Forand D, Wolf M, Tyus C, Barney J, Kellogg L, Simon MA, Kerr G, Wells KL, Younes S, Mortimer NT, Olesnicky EC, Killian DJ - G3 (Bethesda) (2015)

Cytoplasmic localization of CGH-1, CPB-3, LARP-5, and SUP-26 in PVD neurons. cDNAs for RNA-binding protein (RBP) genes indicated were fused to green fluorescent protein (GFP) under the control of a PVD-specific promoter and reveal cytoplasmic localization. PVDs were marked by ser-2prom3::myr-mCherry. Arrowheads indicate GFP-positive particles within dendrites. Bar = 5 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390579&req=5

fig6: Cytoplasmic localization of CGH-1, CPB-3, LARP-5, and SUP-26 in PVD neurons. cDNAs for RNA-binding protein (RBP) genes indicated were fused to green fluorescent protein (GFP) under the control of a PVD-specific promoter and reveal cytoplasmic localization. PVDs were marked by ser-2prom3::myr-mCherry. Arrowheads indicate GFP-positive particles within dendrites. Bar = 5 μm.
Mentions: DDX-17, MBL-1, MTR-4, RSP-3, RSP-6, SET-2, and Y55F3AM.3 translation fusions to GFP show clear nuclear expression (Figure 5). This finding is consistent with these RBPs playing roles in RNA processing within the nucleus (see the section Discussion). Conversely, we find that CGH-1, CPB-3, LARP-5, and SUP-26 translational fusions to GFP are localized to the cytoplasm (Figure 6). More specifically, CGH-1::GFP is enriched in the perinuclear region and small puncta are present throughout the cytoplasm, including particles in dendrites (Figure 6). Similarly, CPB-3::GFP also is found in puncta in the cytoplasm of the cell body and dendrites. However, CPB-3 puncta are substantially larger and lack perinuclear enrichment (Figure 6). Unlike CGH-1 and CPB-3, SUP-26::GFP is restricted to the cytoplasm of the cell body and is not found in dendrites. SUP-26 has a striking, perinuclear enrichment with intermediate-sized puncta, relative to CGH-1 and CPB-3, located further from the nucleus but still within the cell body (Figure 6). Finally, LARP-5::GFP is diffuse throughout the cytoplasm, including the dendrites, and the nucleus and does not localize to discrete puncta (Figure 6).

Bottom Line: Homologs of each of these genes were previously identified as important in the Drosophila melanogaster dendritic arborization sensory neurons.Our results suggest that RNA processing, mRNA localization, mRNA stability, and translational control are all important mechanisms that contribute to dendrite morphogenesis, and we present a conserved set of RNA-binding proteins that regulate these processes in diverse animal species.Furthermore, homologs of these genes are expressed in the human brain, suggesting that these RNA-binding proteins are candidate regulators of dendrite development in humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Colorado College, Colorado Springs, Colorado 80903.

Show MeSH
Related in: MedlinePlus