Limits...
Conserved RNA-binding proteins required for dendrite morphogenesis in Caenorhabditis elegans sensory neurons.

Antonacci S, Forand D, Wolf M, Tyus C, Barney J, Kellogg L, Simon MA, Kerr G, Wells KL, Younes S, Mortimer NT, Olesnicky EC, Killian DJ - G3 (Bethesda) (2015)

Bottom Line: Homologs of each of these genes were previously identified as important in the Drosophila melanogaster dendritic arborization sensory neurons.Our results suggest that RNA processing, mRNA localization, mRNA stability, and translational control are all important mechanisms that contribute to dendrite morphogenesis, and we present a conserved set of RNA-binding proteins that regulate these processes in diverse animal species.Furthermore, homologs of these genes are expressed in the human brain, suggesting that these RNA-binding proteins are candidate regulators of dendrite development in humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Colorado College, Colorado Springs, Colorado 80903.

Show MeSH

Related in: MedlinePlus

Loss or reduction of RNA-binding protein (RBP) genes results in a decrease in dendritic termini in PVD neurons. (A) PVD dendritic tree morphology includes primary (1°) branches extending from the cell body (CB) and a series of perpendicular secondary (2°), tertiary (3°), and quaternary (4°) branches. (B) Animals carrying a green fluorescent protein (GFP) marker for PVD neurons and a mutation in the RBP gene indicated have reduced dendritic termini compared with the control (ctl). (C) Animals carrying a GFP marker for PVD neurons and treated with RNA interference (RNAi) for the genes indicated have reduced dendritic termini compared with the control. mec-3(RNAi) is a positive control for RNAi and reduced dendrite phenotypes. Posterior is to the right in all images. Bar = 25 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4390579&req=5

fig1: Loss or reduction of RNA-binding protein (RBP) genes results in a decrease in dendritic termini in PVD neurons. (A) PVD dendritic tree morphology includes primary (1°) branches extending from the cell body (CB) and a series of perpendicular secondary (2°), tertiary (3°), and quaternary (4°) branches. (B) Animals carrying a green fluorescent protein (GFP) marker for PVD neurons and a mutation in the RBP gene indicated have reduced dendritic termini compared with the control (ctl). (C) Animals carrying a GFP marker for PVD neurons and treated with RNA interference (RNAi) for the genes indicated have reduced dendritic termini compared with the control. mec-3(RNAi) is a positive control for RNAi and reduced dendrite phenotypes. Posterior is to the right in all images. Bar = 25 μm.

Mentions: The C. elegans PVD neuron is an excellent model for the molecular genetic investigation of dendrite morphogenesis. The bilateral PVDs, which are located between the epidermis and the body wall muscles, have extensively branched dendritic trees that function as mechanoreceptors, nociceptors, proprioceptors, and cold temperature receptors (Way and Chalfie 1989; Halevi et al. 2002; Tsalik and Hobert 2003; Oren-Suissa et al. 2010; Smith et al. 2010; Albeg et al. 2011; Chatzigeorgiou and Schafer 2011). PVD dendritic trees are stereotypic with primary (1°) branches that project anteriorly and posteriorly from the cell body and menorah- or candelabra-shaped structures extending from the primary branches, which include an orthogonal series of secondary (2°), tertiary (3°), and quaternary (4°) branches (Oren-Suissa et al. 2010; Smith et al. 2010; Figure 1A). Thus, PVD function and morphology are similar to Drosophila da neurons and mammalian polymodal nociceptors (Albeg et al. 2011).


Conserved RNA-binding proteins required for dendrite morphogenesis in Caenorhabditis elegans sensory neurons.

Antonacci S, Forand D, Wolf M, Tyus C, Barney J, Kellogg L, Simon MA, Kerr G, Wells KL, Younes S, Mortimer NT, Olesnicky EC, Killian DJ - G3 (Bethesda) (2015)

Loss or reduction of RNA-binding protein (RBP) genes results in a decrease in dendritic termini in PVD neurons. (A) PVD dendritic tree morphology includes primary (1°) branches extending from the cell body (CB) and a series of perpendicular secondary (2°), tertiary (3°), and quaternary (4°) branches. (B) Animals carrying a green fluorescent protein (GFP) marker for PVD neurons and a mutation in the RBP gene indicated have reduced dendritic termini compared with the control (ctl). (C) Animals carrying a GFP marker for PVD neurons and treated with RNA interference (RNAi) for the genes indicated have reduced dendritic termini compared with the control. mec-3(RNAi) is a positive control for RNAi and reduced dendrite phenotypes. Posterior is to the right in all images. Bar = 25 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390579&req=5

fig1: Loss or reduction of RNA-binding protein (RBP) genes results in a decrease in dendritic termini in PVD neurons. (A) PVD dendritic tree morphology includes primary (1°) branches extending from the cell body (CB) and a series of perpendicular secondary (2°), tertiary (3°), and quaternary (4°) branches. (B) Animals carrying a green fluorescent protein (GFP) marker for PVD neurons and a mutation in the RBP gene indicated have reduced dendritic termini compared with the control (ctl). (C) Animals carrying a GFP marker for PVD neurons and treated with RNA interference (RNAi) for the genes indicated have reduced dendritic termini compared with the control. mec-3(RNAi) is a positive control for RNAi and reduced dendrite phenotypes. Posterior is to the right in all images. Bar = 25 μm.
Mentions: The C. elegans PVD neuron is an excellent model for the molecular genetic investigation of dendrite morphogenesis. The bilateral PVDs, which are located between the epidermis and the body wall muscles, have extensively branched dendritic trees that function as mechanoreceptors, nociceptors, proprioceptors, and cold temperature receptors (Way and Chalfie 1989; Halevi et al. 2002; Tsalik and Hobert 2003; Oren-Suissa et al. 2010; Smith et al. 2010; Albeg et al. 2011; Chatzigeorgiou and Schafer 2011). PVD dendritic trees are stereotypic with primary (1°) branches that project anteriorly and posteriorly from the cell body and menorah- or candelabra-shaped structures extending from the primary branches, which include an orthogonal series of secondary (2°), tertiary (3°), and quaternary (4°) branches (Oren-Suissa et al. 2010; Smith et al. 2010; Figure 1A). Thus, PVD function and morphology are similar to Drosophila da neurons and mammalian polymodal nociceptors (Albeg et al. 2011).

Bottom Line: Homologs of each of these genes were previously identified as important in the Drosophila melanogaster dendritic arborization sensory neurons.Our results suggest that RNA processing, mRNA localization, mRNA stability, and translational control are all important mechanisms that contribute to dendrite morphogenesis, and we present a conserved set of RNA-binding proteins that regulate these processes in diverse animal species.Furthermore, homologs of these genes are expressed in the human brain, suggesting that these RNA-binding proteins are candidate regulators of dendrite development in humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Colorado College, Colorado Springs, Colorado 80903.

Show MeSH
Related in: MedlinePlus