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Gene regulation by H-NS as a function of growth conditions depends on chromosomal position in Escherichia coli.

Brambilla E, Sclavi B - G3 (Bethesda) (2015)

Bottom Line: Cellular adaptation to changing environmental conditions requires the coordinated regulation of expression of large sets of genes by global regulatory factors such as nucleoid associated proteins.Our results show that the activity of the Phns promoter depends on whether it is placed within the AT-rich regions of the genome that are known to be bound preferentially by H-NS.Genomic position can thus play a significant role in the adaptation of the cells to environmental changes, providing a fitness advantage that can explain the selection of a gene's position during evolution.

View Article: PubMed Central - PubMed

Affiliation: LBPA, UMR 8113 du CNRS, Ecole Normale Supérieure de Cachan, Cachan, France School of Engineering and Science, Jacobs University Bremen, Bremen, Germany.

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The change in YFP concentration as a function of growth rate shows an increase in protein dilution at faster growth and a growth rate dependence in the difference between RT and LT. Protein concentration for samples growing in M9 minimal media supplemented with various concentration of glucose and casamino acids at 30°. The error bars are the SEM resulting from three independent experiments. Data were taken at mid exponential phase. At fast growth there is a low YFP concentration due to a faster dilution rate. For the LT strain (white squares) the YFP concentration values are low also at slow growth, indicating a stronger repression in this position than at the others. LM, left medium; LO, left origin; LT, left terminus; RM, right medium; RO, right origin; RT, right terminus; YFP, yellow fluorescent protein.
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fig5: The change in YFP concentration as a function of growth rate shows an increase in protein dilution at faster growth and a growth rate dependence in the difference between RT and LT. Protein concentration for samples growing in M9 minimal media supplemented with various concentration of glucose and casamino acids at 30°. The error bars are the SEM resulting from three independent experiments. Data were taken at mid exponential phase. At fast growth there is a low YFP concentration due to a faster dilution rate. For the LT strain (white squares) the YFP concentration values are low also at slow growth, indicating a stronger repression in this position than at the others. LM, left medium; LO, left origin; LT, left terminus; RM, right medium; RO, right origin; RT, right terminus; YFP, yellow fluorescent protein.

Mentions: Schematic representation of the Phns promoter and of the insertions in the E. coli chromosome. (A) The boxes indicate the binding sites for different proteins in the Phns promoter region (black for FIS, gray for H-NS, white for CspA) as derived from the literature (La Teana et al. 1991; Ueguchi et al. 1993; Falconi et al. 1996). Stars indicate the H-NS high-affinity DNA binding sites (Lang et al. 2007). The -10, -35 regions and the transcription starting site, +1, also are annotated. (B) The promoter-yfp unit is flanked by two T1 terminators from the E. coli rrnB coding sequence. (C) Representation of the six different mirror sites on the E. coli chromosome in which the yfp gene was inserted under the control of the Phns promoter next to the gene conferring resistance to chloramphenicol. The symbols used here are the ones used to indicate these positions in Figure 2B, Figure 5 and Supporting Information, Figure S6. Details about the insertion positions can be found in Table 1. CspA, Cold shock protein A; FIS, factor for inversion stimulation; H-NS, histone-like nucleoid-structuring protein.


Gene regulation by H-NS as a function of growth conditions depends on chromosomal position in Escherichia coli.

Brambilla E, Sclavi B - G3 (Bethesda) (2015)

The change in YFP concentration as a function of growth rate shows an increase in protein dilution at faster growth and a growth rate dependence in the difference between RT and LT. Protein concentration for samples growing in M9 minimal media supplemented with various concentration of glucose and casamino acids at 30°. The error bars are the SEM resulting from three independent experiments. Data were taken at mid exponential phase. At fast growth there is a low YFP concentration due to a faster dilution rate. For the LT strain (white squares) the YFP concentration values are low also at slow growth, indicating a stronger repression in this position than at the others. LM, left medium; LO, left origin; LT, left terminus; RM, right medium; RO, right origin; RT, right terminus; YFP, yellow fluorescent protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390576&req=5

fig5: The change in YFP concentration as a function of growth rate shows an increase in protein dilution at faster growth and a growth rate dependence in the difference between RT and LT. Protein concentration for samples growing in M9 minimal media supplemented with various concentration of glucose and casamino acids at 30°. The error bars are the SEM resulting from three independent experiments. Data were taken at mid exponential phase. At fast growth there is a low YFP concentration due to a faster dilution rate. For the LT strain (white squares) the YFP concentration values are low also at slow growth, indicating a stronger repression in this position than at the others. LM, left medium; LO, left origin; LT, left terminus; RM, right medium; RO, right origin; RT, right terminus; YFP, yellow fluorescent protein.
Mentions: Schematic representation of the Phns promoter and of the insertions in the E. coli chromosome. (A) The boxes indicate the binding sites for different proteins in the Phns promoter region (black for FIS, gray for H-NS, white for CspA) as derived from the literature (La Teana et al. 1991; Ueguchi et al. 1993; Falconi et al. 1996). Stars indicate the H-NS high-affinity DNA binding sites (Lang et al. 2007). The -10, -35 regions and the transcription starting site, +1, also are annotated. (B) The promoter-yfp unit is flanked by two T1 terminators from the E. coli rrnB coding sequence. (C) Representation of the six different mirror sites on the E. coli chromosome in which the yfp gene was inserted under the control of the Phns promoter next to the gene conferring resistance to chloramphenicol. The symbols used here are the ones used to indicate these positions in Figure 2B, Figure 5 and Supporting Information, Figure S6. Details about the insertion positions can be found in Table 1. CspA, Cold shock protein A; FIS, factor for inversion stimulation; H-NS, histone-like nucleoid-structuring protein.

Bottom Line: Cellular adaptation to changing environmental conditions requires the coordinated regulation of expression of large sets of genes by global regulatory factors such as nucleoid associated proteins.Our results show that the activity of the Phns promoter depends on whether it is placed within the AT-rich regions of the genome that are known to be bound preferentially by H-NS.Genomic position can thus play a significant role in the adaptation of the cells to environmental changes, providing a fitness advantage that can explain the selection of a gene's position during evolution.

View Article: PubMed Central - PubMed

Affiliation: LBPA, UMR 8113 du CNRS, Ecole Normale Supérieure de Cachan, Cachan, France School of Engineering and Science, Jacobs University Bremen, Bremen, Germany.

Show MeSH
Related in: MedlinePlus