Global diversity lines - a five-continent reference panel of sequenced Drosophila melanogaster strains.
Bottom Line: Another key feature of these strains is their widespread geographic origin, coming from Beijing, Ithaca, Netherlands, Tasmania, and Zimbabwe.We found 83 segregating inversions among the lines, and as expected these were especially abundant in the African sample.We anticipate that this will make a useful addition to the set of reference D. melanogaster strains, thanks to its geographic structuring and unusually high level of genetic diversity.
Affiliation: Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853.Show MeSH
Related in: MedlinePlus
Mentions: We next investigated the distribution of variant calls with respect to gene annotation. Variants with high predicted impact and located in exons were least likely to be retained in the variant call pipeline (Figure 3), indicating that as call quality increased due to recalibration and filtering, the false-positive rate of high impact sites decreased. Additionally, we observe a clear mod-3 pattern in small indels located in coding regions, which is magnified by the recalibration and filtering steps (Figure S7). These patterns of change in the distribution of variant annotations indicate the improved quality of the variant calls at each step of the pipeline.
Affiliation: Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853.