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Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.

Huang Y, Ng FS, Jackson FR - G3 (Bethesda) (2015)

Bottom Line: These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior.Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis.Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.

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Gene ontology (GO) relationships for adult-enriched genes/proteins involved in vesicle-mediated transport and vesicle secretion. The expression of genes encoding these proteins is overrepresented in adult astrocytes, as indicated by the GO Toolset (Princeton University). Note that this figure does not include other factors, such as Rop, syntaxins, sec homologs, Rab proteins, and others, which exhibit astrocyte-enrichment in both larvae and adults.
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fig5: Gene ontology (GO) relationships for adult-enriched genes/proteins involved in vesicle-mediated transport and vesicle secretion. The expression of genes encoding these proteins is overrepresented in adult astrocytes, as indicated by the GO Toolset (Princeton University). Note that this figure does not include other factors, such as Rop, syntaxins, sec homologs, Rab proteins, and others, which exhibit astrocyte-enrichment in both larvae and adults.

Mentions: To asses GO overrepresentation in the genes with adult-specific enrichment, we again used the GO Toolset (Princeton University). Using a cutoff of P < 0.01, we determined that two major GO terms were overrepresented in the adult genes: chromatin modification and vesicle-mediated transport (Table S8). The latter category includes genes important for intracellular transport and neurosecretion. Figure 5 illustrates the relationships between GO categories and genes that fall under vesicle-mediated transport. Many of these genes encode factors important for vesicle secretion. We note that this figure only shows genes found to have adult-specific enrichment. There are many other factors required for vesicular trafficking or secretion that show astrocyte enrichment in larvae and in adults (Table S3 and Table S4). Overrepresentation of this category of genes strongly suggests an important function for astrocyte secretion in the adult fly CNS.


Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.

Huang Y, Ng FS, Jackson FR - G3 (Bethesda) (2015)

Gene ontology (GO) relationships for adult-enriched genes/proteins involved in vesicle-mediated transport and vesicle secretion. The expression of genes encoding these proteins is overrepresented in adult astrocytes, as indicated by the GO Toolset (Princeton University). Note that this figure does not include other factors, such as Rop, syntaxins, sec homologs, Rab proteins, and others, which exhibit astrocyte-enrichment in both larvae and adults.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390571&req=5

fig5: Gene ontology (GO) relationships for adult-enriched genes/proteins involved in vesicle-mediated transport and vesicle secretion. The expression of genes encoding these proteins is overrepresented in adult astrocytes, as indicated by the GO Toolset (Princeton University). Note that this figure does not include other factors, such as Rop, syntaxins, sec homologs, Rab proteins, and others, which exhibit astrocyte-enrichment in both larvae and adults.
Mentions: To asses GO overrepresentation in the genes with adult-specific enrichment, we again used the GO Toolset (Princeton University). Using a cutoff of P < 0.01, we determined that two major GO terms were overrepresented in the adult genes: chromatin modification and vesicle-mediated transport (Table S8). The latter category includes genes important for intracellular transport and neurosecretion. Figure 5 illustrates the relationships between GO categories and genes that fall under vesicle-mediated transport. Many of these genes encode factors important for vesicle secretion. We note that this figure only shows genes found to have adult-specific enrichment. There are many other factors required for vesicular trafficking or secretion that show astrocyte enrichment in larvae and in adults (Table S3 and Table S4). Overrepresentation of this category of genes strongly suggests an important function for astrocyte secretion in the adult fly CNS.

Bottom Line: These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior.Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis.Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.

Show MeSH
Related in: MedlinePlus