Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.
Bottom Line: These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior.Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis.Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.
Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.Show MeSH
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Mentions: To asses GO overrepresentation in the genes with adult-specific enrichment, we again used the GO Toolset (Princeton University). Using a cutoff of P < 0.01, we determined that two major GO terms were overrepresented in the adult genes: chromatin modification and vesicle-mediated transport (Table S8). The latter category includes genes important for intracellular transport and neurosecretion. Figure 5 illustrates the relationships between GO categories and genes that fall under vesicle-mediated transport. Many of these genes encode factors important for vesicle secretion. We note that this figure only shows genes found to have adult-specific enrichment. There are many other factors required for vesicular trafficking or secretion that show astrocyte enrichment in larvae and in adults (Table S3 and Table S4). Overrepresentation of this category of genes strongly suggests an important function for astrocyte secretion in the adult fly CNS.
Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.