Limits...
Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.

Huang Y, Ng FS, Jackson FR - G3 (Bethesda) (2015)

Bottom Line: These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior.Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis.Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.

Show MeSH

Related in: MedlinePlus

Enhancer trap-driven GFP expression for GAT and CG14141. Strains NP5633 (Kyoto stock collection) and 46117 (Janelia Farm) with Gal4 insertions near CG14141 and in or near GAT were used to drive nuclear GFP. Brains were stained with anti-GFP to detect enhancer trap expression and anti-Repo to reveal all glial cells. As shown in the merge images, both Gal4 drivers express in a subset of Repo-positive glial cells (yellow), consistent with expression in astrocytes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4390571&req=5

fig4: Enhancer trap-driven GFP expression for GAT and CG14141. Strains NP5633 (Kyoto stock collection) and 46117 (Janelia Farm) with Gal4 insertions near CG14141 and in or near GAT were used to drive nuclear GFP. Brains were stained with anti-GFP to detect enhancer trap expression and anti-Repo to reveal all glial cells. As shown in the merge images, both Gal4 drivers express in a subset of Repo-positive glial cells (yellow), consistent with expression in astrocytes.

Mentions: As already mentioned, many of these genes are known to be expressed in fly astrocytes, based on previous studies. Making use of enhancer trap Gal4 insertions in or near the genes encoding GAT (known astrocyte expression) and a novel enriched factor (CG14141), we examined the expression pattern relative to Repo, a pan-glial factor. Both genes are expressed in a subset of Repo-positive glial cells of the larval brain lobes, consistent with astrocyte expression (Figure 4).


Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.

Huang Y, Ng FS, Jackson FR - G3 (Bethesda) (2015)

Enhancer trap-driven GFP expression for GAT and CG14141. Strains NP5633 (Kyoto stock collection) and 46117 (Janelia Farm) with Gal4 insertions near CG14141 and in or near GAT were used to drive nuclear GFP. Brains were stained with anti-GFP to detect enhancer trap expression and anti-Repo to reveal all glial cells. As shown in the merge images, both Gal4 drivers express in a subset of Repo-positive glial cells (yellow), consistent with expression in astrocytes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390571&req=5

fig4: Enhancer trap-driven GFP expression for GAT and CG14141. Strains NP5633 (Kyoto stock collection) and 46117 (Janelia Farm) with Gal4 insertions near CG14141 and in or near GAT were used to drive nuclear GFP. Brains were stained with anti-GFP to detect enhancer trap expression and anti-Repo to reveal all glial cells. As shown in the merge images, both Gal4 drivers express in a subset of Repo-positive glial cells (yellow), consistent with expression in astrocytes.
Mentions: As already mentioned, many of these genes are known to be expressed in fly astrocytes, based on previous studies. Making use of enhancer trap Gal4 insertions in or near the genes encoding GAT (known astrocyte expression) and a novel enriched factor (CG14141), we examined the expression pattern relative to Repo, a pan-glial factor. Both genes are expressed in a subset of Repo-positive glial cells of the larval brain lobes, consistent with astrocyte expression (Figure 4).

Bottom Line: These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior.Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis.Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.

Show MeSH
Related in: MedlinePlus