Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.
Bottom Line: These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior.Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis.Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.
Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.Show MeSH
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Mentions: Three biological replicates were sequenced for the TRAP-IP and total RNA samples. For all TRAP RNA samples, average read numbers ranged from 0 to ∼315,000 for individual genes. Of these astrocyte-expressed genes, 9107 were represented by 20 or more reads in all three samples. For the total RNA samples, the reads averaged 0 to ∼426,000, and 9725 genes were represented by 20 or more reads. All samples had a mean quality score of >30. Figure 2 shows scatter plots indicating the reproducibility of RNA-seq results for the TRAP IP and total RNA replicate samples. A spearman rank correlation analysis indicated that there was greater than a 95% correlation between samples with regard to expression values.
Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.