Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles.
Bottom Line: These include genes important for metabolism and energy production, translation, chromatin modification, protein glycosylation, neuropeptide signaling, immune responses, vesicle-mediated trafficking or secretion, and the regulation of behavior.Among these functional classes, the expression of genes important for chromatin modification and vesicle-mediated trafficking or secretion is overrepresented in adult astrocytes based on Gene Ontology analysis.Certain genes with selective adult enrichment may mediate functions specific to this stage or may be important for the differentiation or maintenance of adult astrocytes, with the latter perhaps contributing to population heterogeneity.
Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.Show MeSH
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Mentions: We performed larval TRAP profiling using a previously described astrocyte Gal4 driver strain (alrm-Gal4) (Doherty et al. 2009). The alrm-Gal4 driver was combined with a Drosophila transgene encoding a tagged large ribosomal subunit (UAS-EGFP-L10a) (Huang et al. 2013) to generate flies specifically expressing EGFP-L10a in astrocytes of the larval nervous system (brain and ventral nerve cord; Figure 1A). As shown in Figure 1, EGFP-L10a could be detected in glial cells of the larval brain lobes (Figure 1, B and C) and ventral nerve cord (Figure 1, B and D). In both locations, EGFP-L10a was observed to be cytoplasmic, as determined by straining with the Repo nuclear marker (Figure 1, C and D). Expression of alrm-Gal4-driven EGFP-L10a throughout development did not grossly affect astrocyte cell morphology (Figure 1 and data not shown), nor did it affect viability or activity level or cause circadian arrhythmicity for adult animals (Supporting Information, Figure S1).
Affiliation: Department of Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts 02111.