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Heritable variation in courtship patterns in Drosophila melanogaster.

Gaertner BE, Ruedi EA, McCoy LJ, Moore JM, Wolfner MF, Mackay TF - G3 (Bethesda) (2015)

Bottom Line: We found heritable variation along the expected trajectory for courtship behaviors, including the tendency to initiate courtship and rate of progression through courtship, suggesting a genetic basis to male modulation of courtship behavior based on feedback from unrelated, outbred, and genetically identical females.We assessed the genetic basis of variation of the transition with the greatest heritability--from copulation to no engagement with the female--and identified variants in Serrate and Furin 1 as well as many other polymorphisms on the chromosome 3R associated with this transition.Our findings suggest that courtship is a highly dynamic behavior with both social and genetic inputs, and that males may play an important role in courtship initiation and duration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, W. M. Keck Center for Behavioral Biology and Program in Genetics, North Carolina State University, Raleigh, North Carolina 27695-7614.

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Related in: MedlinePlus

Copulatron schematic (expanded view). The copulatron is constructed of five layers: a CO2 pad (bottom), the lower chamber for D. melanogaster Genetic Reference Panel males, a thick opaque divider, the upper layer for tester (Or × Sam) females, and a clear lid. For simplicity, alignment posts, food, and a CO2 hookup are not shown. The divider is pulled out from between the two chambers to initiate the male mating progression assay. Copulatrons are modeled after Drapeau and Long (2000).
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fig1: Copulatron schematic (expanded view). The copulatron is constructed of five layers: a CO2 pad (bottom), the lower chamber for D. melanogaster Genetic Reference Panel males, a thick opaque divider, the upper layer for tester (Or × Sam) females, and a clear lid. For simplicity, alignment posts, food, and a CO2 hookup are not shown. The divider is pulled out from between the two chambers to initiate the male mating progression assay. Copulatrons are modeled after Drapeau and Long (2000).

Mentions: MMP (Ruedi and Hughes 2008) was observed using 8-arena “copulatrons” modeled after Drapeau and Long (2000) from Spring 2010 to Spring 2011 in five blocks (Figure 1). A total of 166 DGRP lines were assayed. Each line was assigned randomly to a block, and 10−15 males per DGRP line were assayed. Within each block, males were assigned randomly to trials and arenas of the copulatron to minimize the environmental variance; thus, multiple lines were assessed multiple days in a randomized manner. Approximately 18 hr before the start of the trial, males were lightly anesthetized with CO2 and placed in the lower portion of an arena. An opaque sheet of plastic was added, and virgin females (also lightly anesthetized with CO2) were placed in the upper portion of the arena. A clear Plexiglas lid was placed on the arena, and the flies were allowed to recover from anesthesia overnight at 25°. Both arena compartments contained fly food media to prevent starvation, desiccation, and general stress.


Heritable variation in courtship patterns in Drosophila melanogaster.

Gaertner BE, Ruedi EA, McCoy LJ, Moore JM, Wolfner MF, Mackay TF - G3 (Bethesda) (2015)

Copulatron schematic (expanded view). The copulatron is constructed of five layers: a CO2 pad (bottom), the lower chamber for D. melanogaster Genetic Reference Panel males, a thick opaque divider, the upper layer for tester (Or × Sam) females, and a clear lid. For simplicity, alignment posts, food, and a CO2 hookup are not shown. The divider is pulled out from between the two chambers to initiate the male mating progression assay. Copulatrons are modeled after Drapeau and Long (2000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390569&req=5

fig1: Copulatron schematic (expanded view). The copulatron is constructed of five layers: a CO2 pad (bottom), the lower chamber for D. melanogaster Genetic Reference Panel males, a thick opaque divider, the upper layer for tester (Or × Sam) females, and a clear lid. For simplicity, alignment posts, food, and a CO2 hookup are not shown. The divider is pulled out from between the two chambers to initiate the male mating progression assay. Copulatrons are modeled after Drapeau and Long (2000).
Mentions: MMP (Ruedi and Hughes 2008) was observed using 8-arena “copulatrons” modeled after Drapeau and Long (2000) from Spring 2010 to Spring 2011 in five blocks (Figure 1). A total of 166 DGRP lines were assayed. Each line was assigned randomly to a block, and 10−15 males per DGRP line were assayed. Within each block, males were assigned randomly to trials and arenas of the copulatron to minimize the environmental variance; thus, multiple lines were assessed multiple days in a randomized manner. Approximately 18 hr before the start of the trial, males were lightly anesthetized with CO2 and placed in the lower portion of an arena. An opaque sheet of plastic was added, and virgin females (also lightly anesthetized with CO2) were placed in the upper portion of the arena. A clear Plexiglas lid was placed on the arena, and the flies were allowed to recover from anesthesia overnight at 25°. Both arena compartments contained fly food media to prevent starvation, desiccation, and general stress.

Bottom Line: We found heritable variation along the expected trajectory for courtship behaviors, including the tendency to initiate courtship and rate of progression through courtship, suggesting a genetic basis to male modulation of courtship behavior based on feedback from unrelated, outbred, and genetically identical females.We assessed the genetic basis of variation of the transition with the greatest heritability--from copulation to no engagement with the female--and identified variants in Serrate and Furin 1 as well as many other polymorphisms on the chromosome 3R associated with this transition.Our findings suggest that courtship is a highly dynamic behavior with both social and genetic inputs, and that males may play an important role in courtship initiation and duration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, W. M. Keck Center for Behavioral Biology and Program in Genetics, North Carolina State University, Raleigh, North Carolina 27695-7614.

Show MeSH
Related in: MedlinePlus