The phage Mu middle promoter Pm contains a partial UP element.
Bottom Line: The results demonstrated that mutations upstream of -57 had no effect on Pm activity in vivo, assayed by expression of lacZ fused downstream of a wild-type or mutant Pm.In DNase I footprinting and gel mobility shift assays, paired mutations at positions -55 and -54 did not affect Mor binding but decreased the synergistic binding of Mor with histidine tagged α (His-α), indicating that His-α binds to Pm in a sequence- and/or structure-specific manner.Taken together, these results demonstrate that Pm has a strong proximal UP element subsite, but lacks a distal subsite.
Affiliation: Department of Microbiology, Immunology & Biochemistry, University of Tennessee Health Science Center, Memphis, Tennessee 38163.Show MeSH
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Mentions: Because mutations close to the Mor binding site (−51 to −36) could affect Pm activity by altering Mor binding, gel mobility shift assays were performed to examine Mor binding to mutant Pm DNA fragments. All of the mutants had substantial Mor binding as shown in the representative gels presented in Figure 4.
Affiliation: Department of Microbiology, Immunology & Biochemistry, University of Tennessee Health Science Center, Memphis, Tennessee 38163.