The transcriptional stress response of Candida albicans to weak organic acids.
Bottom Line: Genes commonly up-regulated by WOAs were enriched in several iron transporters, which was associated with an overall decrease in intracellular iron concentrations.Moreover, chronic exposure to any WOA lead to down-regulation of RNA synthesis and ribosome biogenesis genes, which resulted in significant reduction of total RNA levels and of ribosomal RNA in particular.In conclusion, this study suggests that gastrointestinal microbiota might directly influence C. albicans physiology via production of WOAs, with possible implications of how this fungus interacts with its host in both health and disease.
Affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore.Show MeSH
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Mentions: The GO enrichment analysis also revealed a distinctive down-regulation of genes involved in RNA synthesis and ribosome biogenesis (Figure 2B), most especially during chronic exposure to WOAs. In accordance, we noticed during sample preparation a significant decrease in the yield of total RNA extracted per unit biomass between WOA-treated and -untreated samples (~50%) as well as between late and early time points (up to ~90%) (Figure 5A). Moreover, WOA treatment caused a significant reduction in the relative abundance of ribosomal RNA over total RNA (Figure 5B). These data suggest that prolonged exposure of C. albicans to WOAs leads to a general down-regulation of both transcription and translation.
Affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore.