The transcriptional stress response of Candida albicans to weak organic acids.
Bottom Line: Genes commonly up-regulated by WOAs were enriched in several iron transporters, which was associated with an overall decrease in intracellular iron concentrations.Moreover, chronic exposure to any WOA lead to down-regulation of RNA synthesis and ribosome biogenesis genes, which resulted in significant reduction of total RNA levels and of ribosomal RNA in particular.In conclusion, this study suggests that gastrointestinal microbiota might directly influence C. albicans physiology via production of WOAs, with possible implications of how this fungus interacts with its host in both health and disease.
Affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore.Show MeSH
Related in: MedlinePlus
Mentions: To more systematically investigate the similarity between the gene expression profiles induced by the different WOAs at the different time points, we evaluated the degree of overlap between all lists of up- and down-regulated genes obtained in this study (Figure 3A and Figure S4A). By this analysis, we detected highly significant overlaps between different WOA responses, many of which shared >40% of genes in common and some of which as high as >80% (i.e., genes up-regulated by propionic or by butyric acid at T3 and T4). More precisely, genes up-regulated at any given time point in response to any given WOA displayed relatively high overlap with genes up-regulated at the same or at any other time point in response to the same or any other WOA (Figure 3A and Figure S4A). A similar picture was found for genes down-regulated by WOAs.
Affiliation: Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore.