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Identification of eQTLs for hepatic Xbp1s and Socs3 gene expression in mice fed a high-fat, high-caloric diet.

Pasricha S, Kenney-Hunt J, Anderson K, Jafari N, Hall RA, Lammert F, Cheverud J, Green RM - G3 (Bethesda) (2015)

Bottom Line: We identified two overlapping loci for Xbp1s and Socs3 on Chr 1 (164.0-185.4 Mb and 174.4-190.5 Mb, respectively) and Chr 11 (41.1-73.1 Mb and 44.0-68.6 Mb, respectively), and an additional locus for Socs3 on Chr 12 (109.9-117.4 Mb).In addition, we replicated the eQTLs on Chr 1 and Chr 12 (LOD scores ≥3.5) using mice from the BXD murine reference panel challenged with CCl4 to induce chronic liver injury and fibrosis.Identification of the genes for these eQTLs will lead to a better understanding of the genetic factors responsible for NAFLD and potentially other hepatic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Northwestern University, Chicago, Illinois Department of Internal Medicine, University of North Carolina, Chapel Hill, North Carolina.

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Hepatic mRNA expression of Xbp1s and Socs3 in A/J, C57BL/6, and F1(A/J × C57BL/6J) mice. Mice were fed a 60% high-fat, high-caloric (HFHC) diet for 8 wk and hepatic mRNA expression of (A) Xbp1s and (B) Socs3 measured using RT-PCR. RLU, random light units. Mean ± SEM. A/J (white), C57BL/6J (black), F1(A/J × C57BL/6J) (gray) mice; ap < 0.05 vs. C57BL/6J; bp < 0.05 vs. A/J mice; cp < 0.05 vs. C57BL/6J mice (n = 8 for all groups).
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fig1: Hepatic mRNA expression of Xbp1s and Socs3 in A/J, C57BL/6, and F1(A/J × C57BL/6J) mice. Mice were fed a 60% high-fat, high-caloric (HFHC) diet for 8 wk and hepatic mRNA expression of (A) Xbp1s and (B) Socs3 measured using RT-PCR. RLU, random light units. Mean ± SEM. A/J (white), C57BL/6J (black), F1(A/J × C57BL/6J) (gray) mice; ap < 0.05 vs. C57BL/6J; bp < 0.05 vs. A/J mice; cp < 0.05 vs. C57BL/6J mice (n = 8 for all groups).

Mentions: Parental mouse strains C57BL/6J and A/J fed a HFHC diet for 8 wk elicited significant differences in the hepatic gene expression of Xbp1s: 1.0 ± 0.2 vs. 0.4 ± 0.2 in A/J and C57BL/6J mice, respectively (P < 0.05). There was also a similar difference in hepatic Socs3 gene expression: 1.0 ± 0.2 vs. 0.6 ± 0.2 in A/J and C57BL/6J mice, respectively (P < 0.05). Hepatic Xbp1s and Socs3 gene expression in F1 (A/J × C57BL/6J) mice was 0.7 ± 0.1 for Xbp1s and 0.7 ± 0.2 for Socs3 (P < 0.05), both significantly different from A/J mice. Hepatic Xbp1s expression also differed between the F1 (A/J × C57BL/6J) and C57BL/6J mice (Figure 1; n = 8 for all groups). Supporting Information, Table S1 shows the Pearson correlation matrix of the phenotypes Xbp1s and Socs3 in C57BL/6J and A/J mice (as well as for several phenotypes of the metabolic syndrome).


Identification of eQTLs for hepatic Xbp1s and Socs3 gene expression in mice fed a high-fat, high-caloric diet.

Pasricha S, Kenney-Hunt J, Anderson K, Jafari N, Hall RA, Lammert F, Cheverud J, Green RM - G3 (Bethesda) (2015)

Hepatic mRNA expression of Xbp1s and Socs3 in A/J, C57BL/6, and F1(A/J × C57BL/6J) mice. Mice were fed a 60% high-fat, high-caloric (HFHC) diet for 8 wk and hepatic mRNA expression of (A) Xbp1s and (B) Socs3 measured using RT-PCR. RLU, random light units. Mean ± SEM. A/J (white), C57BL/6J (black), F1(A/J × C57BL/6J) (gray) mice; ap < 0.05 vs. C57BL/6J; bp < 0.05 vs. A/J mice; cp < 0.05 vs. C57BL/6J mice (n = 8 for all groups).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390565&req=5

fig1: Hepatic mRNA expression of Xbp1s and Socs3 in A/J, C57BL/6, and F1(A/J × C57BL/6J) mice. Mice were fed a 60% high-fat, high-caloric (HFHC) diet for 8 wk and hepatic mRNA expression of (A) Xbp1s and (B) Socs3 measured using RT-PCR. RLU, random light units. Mean ± SEM. A/J (white), C57BL/6J (black), F1(A/J × C57BL/6J) (gray) mice; ap < 0.05 vs. C57BL/6J; bp < 0.05 vs. A/J mice; cp < 0.05 vs. C57BL/6J mice (n = 8 for all groups).
Mentions: Parental mouse strains C57BL/6J and A/J fed a HFHC diet for 8 wk elicited significant differences in the hepatic gene expression of Xbp1s: 1.0 ± 0.2 vs. 0.4 ± 0.2 in A/J and C57BL/6J mice, respectively (P < 0.05). There was also a similar difference in hepatic Socs3 gene expression: 1.0 ± 0.2 vs. 0.6 ± 0.2 in A/J and C57BL/6J mice, respectively (P < 0.05). Hepatic Xbp1s and Socs3 gene expression in F1 (A/J × C57BL/6J) mice was 0.7 ± 0.1 for Xbp1s and 0.7 ± 0.2 for Socs3 (P < 0.05), both significantly different from A/J mice. Hepatic Xbp1s expression also differed between the F1 (A/J × C57BL/6J) and C57BL/6J mice (Figure 1; n = 8 for all groups). Supporting Information, Table S1 shows the Pearson correlation matrix of the phenotypes Xbp1s and Socs3 in C57BL/6J and A/J mice (as well as for several phenotypes of the metabolic syndrome).

Bottom Line: We identified two overlapping loci for Xbp1s and Socs3 on Chr 1 (164.0-185.4 Mb and 174.4-190.5 Mb, respectively) and Chr 11 (41.1-73.1 Mb and 44.0-68.6 Mb, respectively), and an additional locus for Socs3 on Chr 12 (109.9-117.4 Mb).In addition, we replicated the eQTLs on Chr 1 and Chr 12 (LOD scores ≥3.5) using mice from the BXD murine reference panel challenged with CCl4 to induce chronic liver injury and fibrosis.Identification of the genes for these eQTLs will lead to a better understanding of the genetic factors responsible for NAFLD and potentially other hepatic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Northwestern University, Chicago, Illinois Department of Internal Medicine, University of North Carolina, Chapel Hill, North Carolina.

Show MeSH
Related in: MedlinePlus