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Increased excitability of lateral habenula neurons in adolescent rats following cocaine self-administration.

Neumann PA, Ishikawa M, Otaka M, Huang YH, Schlüter OM, Dong Y - Int. J. Neuropsychopharmacol. (2014)

Bottom Line: However, the mechanisms of this effect are poorly understood.We found two major relevant neuronal subtypes: burst firing neurons and regular spiking neurons.These results may help to explain how cocaine and other drugs negatively impact affect states.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Department (Drs Neumann, Ishikawa, Otaka, and Dong), and Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA (Dr Huang); Molecular Neurobiology, European Neuroscience Institute, Göttingen, Germany (Dr Schlüter). pan23@pitt.edu yandong@pitt.edu.

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The lateral habenula (LHb) and subnuclei regions. (a) Diagram showing the parvocellular and central regions of the medial division of the LHb where cells were recorded (shaded region). 3V, third ventricle; LHbMPc, parvocellular part of the medial division of the LHb; LHbMC, central part of the medial division of the LHb; LHbLMC, magnocellular part of the lateral division of the LHb. (b) Differential interference contrast image of the habenula of a rat brain slice. The LHb region of interest is apparent from natural markings in the surrounding areas and is outlined by a dashed white line in this picture.
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Figure 2: The lateral habenula (LHb) and subnuclei regions. (a) Diagram showing the parvocellular and central regions of the medial division of the LHb where cells were recorded (shaded region). 3V, third ventricle; LHbMPc, parvocellular part of the medial division of the LHb; LHbMC, central part of the medial division of the LHb; LHbLMC, magnocellular part of the lateral division of the LHb. (b) Differential interference contrast image of the habenula of a rat brain slice. The LHb region of interest is apparent from natural markings in the surrounding areas and is outlined by a dashed white line in this picture.

Mentions: Whole-cell current-clamp recordings were made in the LHb, specifically in the parvocellular and central parts of the medial division of LHb (Figure 2a). During recordings, slices were super-fused with artificial cerebrospinal fluid that was heated to 31 to 33°C by passing the solution through a feedback controlled inline heater (Warner Instruments) before entering the recording chamber. Recordings were made under visual guidance (40x, differential interference contrast optics) with micropipettes (2.5–5 MΩ) filled with a potassium-based internal solution containing (in mM): 130 KMeSO3, 10 KCl, 0.4 ethylene glycol tetraacetic acid, 10 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 2.5 Mg-ATP, 0.25 Na-GTP, and 2 MgCl2-6H2O, pH 7.3, 294 mOsm.


Increased excitability of lateral habenula neurons in adolescent rats following cocaine self-administration.

Neumann PA, Ishikawa M, Otaka M, Huang YH, Schlüter OM, Dong Y - Int. J. Neuropsychopharmacol. (2014)

The lateral habenula (LHb) and subnuclei regions. (a) Diagram showing the parvocellular and central regions of the medial division of the LHb where cells were recorded (shaded region). 3V, third ventricle; LHbMPc, parvocellular part of the medial division of the LHb; LHbMC, central part of the medial division of the LHb; LHbLMC, magnocellular part of the lateral division of the LHb. (b) Differential interference contrast image of the habenula of a rat brain slice. The LHb region of interest is apparent from natural markings in the surrounding areas and is outlined by a dashed white line in this picture.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390528&req=5

Figure 2: The lateral habenula (LHb) and subnuclei regions. (a) Diagram showing the parvocellular and central regions of the medial division of the LHb where cells were recorded (shaded region). 3V, third ventricle; LHbMPc, parvocellular part of the medial division of the LHb; LHbMC, central part of the medial division of the LHb; LHbLMC, magnocellular part of the lateral division of the LHb. (b) Differential interference contrast image of the habenula of a rat brain slice. The LHb region of interest is apparent from natural markings in the surrounding areas and is outlined by a dashed white line in this picture.
Mentions: Whole-cell current-clamp recordings were made in the LHb, specifically in the parvocellular and central parts of the medial division of LHb (Figure 2a). During recordings, slices were super-fused with artificial cerebrospinal fluid that was heated to 31 to 33°C by passing the solution through a feedback controlled inline heater (Warner Instruments) before entering the recording chamber. Recordings were made under visual guidance (40x, differential interference contrast optics) with micropipettes (2.5–5 MΩ) filled with a potassium-based internal solution containing (in mM): 130 KMeSO3, 10 KCl, 0.4 ethylene glycol tetraacetic acid, 10 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 2.5 Mg-ATP, 0.25 Na-GTP, and 2 MgCl2-6H2O, pH 7.3, 294 mOsm.

Bottom Line: However, the mechanisms of this effect are poorly understood.We found two major relevant neuronal subtypes: burst firing neurons and regular spiking neurons.These results may help to explain how cocaine and other drugs negatively impact affect states.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Department (Drs Neumann, Ishikawa, Otaka, and Dong), and Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA (Dr Huang); Molecular Neurobiology, European Neuroscience Institute, Göttingen, Germany (Dr Schlüter). pan23@pitt.edu yandong@pitt.edu.

Show MeSH
Related in: MedlinePlus