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Maturation of mast cell progenitors to mucosal mast cells during allergic pulmonary inflammation in mice.

Bankova LG, Dwyer DF, Liu AY, Austen KF, Gurish MF - Mucosal Immunol (2014)

Bottom Line: The resident tracheal CMCs had higher SSC, FcɛRI, and Kit and lower β7-integrin expression than the MMCs.By histology, the MMCs follow similar kinetics to the flow cytometry-identified mature MMCs and are notably persistent for >42 days.Steroid treatment reduced inflammation and MCp influx but had no effect on established MMCs.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.

ABSTRACT
In contrast to resident constitutive mast cells (CMCs), mucosal MCs (MMCs) appear in the lung and trachea of sensitized mice only following inhalation challenge. We monitored the influx and maturation of MCs by their expression of Kit, FcɛRI, β7-integrin and side scatter (SSC) by flow cytometry. Influx of MC progenitors (MCps) (FcɛRI(lo), Kit(int), β7(hi), and SSC(lo)) peaks 1 day after challenges and subsides to baseline by day 7 after challenge. The mature MMCs appear as a distinct population on day 7 and peak at day 14 with higher SSC and FcɛRI expression, but lower β7 and Kit expression. A distinct transitional population is present between 1 and 7 days after challenge. Maturation occurs more rapidly in the trachea. The resident tracheal CMCs had higher SSC, FcɛRI, and Kit and lower β7-integrin expression than the MMCs. By histology, the MMCs follow similar kinetics to the flow cytometry-identified mature MMCs and are notably persistent for >42 days. Steroid treatment reduced inflammation and MCp influx but had no effect on established MMCs. Thus, changes in SSC, FcɛRI, and Kit together with the expression of αE/α4:β7-integrins characterizes the development of induced MMCs from MCps and distinguishes them from resident CMCs in the trachea and large airways.

No MeSH data available.


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Expression of α4, αE, β7, and β1 integrins on the constitutive and inducible MC populations in the trachea. (a) Representative contour plots presenting the expression of α4 and β1 integrins on eMMCs (red) on D1, and MMCs (blue) on D7 after challenges. (b) Mean (±SEM) percentage of cells positive for α4 and β1 integrins in the 2 MC populations, D1 eMMCs, and D7 MMCs as in a.(c) Representative contour plots presenting the expression of αE and β7 integrins colored as in a. (d) Mean (± SEM) percentage of cells positive for αE and β7 integrins in the 2 MC populations colored as in a. Data are from 4 experiments with 4-8 mice per group. **p < 0.001.
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Figure 6: Expression of α4, αE, β7, and β1 integrins on the constitutive and inducible MC populations in the trachea. (a) Representative contour plots presenting the expression of α4 and β1 integrins on eMMCs (red) on D1, and MMCs (blue) on D7 after challenges. (b) Mean (±SEM) percentage of cells positive for α4 and β1 integrins in the 2 MC populations, D1 eMMCs, and D7 MMCs as in a.(c) Representative contour plots presenting the expression of αE and β7 integrins colored as in a. (d) Mean (± SEM) percentage of cells positive for αE and β7 integrins in the 2 MC populations colored as in a. Data are from 4 experiments with 4-8 mice per group. **p < 0.001.

Mentions: Assessment of the different α chain partners of the β7 integrin shows that the eMMCs are 59% positive for α4 on D1 and that this decreases to 4% on the MMCs on D7 (Figure 6a and b). In contrast, the expression of αE is retained at 59% of eMMCs and 66% of MMCs (Figure 6c and d). There is no change in expression of β1 integrin while the β7 integrin expression is decreased from 92% of eMMCs to 68% of MMCs. The majority of MMCs on D7 are positive for both αE and β7 integrin chains. Most of the CMCs in the trachea express β1 integrin and about one half are positive for β7 while only about one third express α4 and very few are positive for the αE integrin chains (data not shown).


Maturation of mast cell progenitors to mucosal mast cells during allergic pulmonary inflammation in mice.

Bankova LG, Dwyer DF, Liu AY, Austen KF, Gurish MF - Mucosal Immunol (2014)

Expression of α4, αE, β7, and β1 integrins on the constitutive and inducible MC populations in the trachea. (a) Representative contour plots presenting the expression of α4 and β1 integrins on eMMCs (red) on D1, and MMCs (blue) on D7 after challenges. (b) Mean (±SEM) percentage of cells positive for α4 and β1 integrins in the 2 MC populations, D1 eMMCs, and D7 MMCs as in a.(c) Representative contour plots presenting the expression of αE and β7 integrins colored as in a. (d) Mean (± SEM) percentage of cells positive for αE and β7 integrins in the 2 MC populations colored as in a. Data are from 4 experiments with 4-8 mice per group. **p < 0.001.
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Related In: Results  -  Collection

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Figure 6: Expression of α4, αE, β7, and β1 integrins on the constitutive and inducible MC populations in the trachea. (a) Representative contour plots presenting the expression of α4 and β1 integrins on eMMCs (red) on D1, and MMCs (blue) on D7 after challenges. (b) Mean (±SEM) percentage of cells positive for α4 and β1 integrins in the 2 MC populations, D1 eMMCs, and D7 MMCs as in a.(c) Representative contour plots presenting the expression of αE and β7 integrins colored as in a. (d) Mean (± SEM) percentage of cells positive for αE and β7 integrins in the 2 MC populations colored as in a. Data are from 4 experiments with 4-8 mice per group. **p < 0.001.
Mentions: Assessment of the different α chain partners of the β7 integrin shows that the eMMCs are 59% positive for α4 on D1 and that this decreases to 4% on the MMCs on D7 (Figure 6a and b). In contrast, the expression of αE is retained at 59% of eMMCs and 66% of MMCs (Figure 6c and d). There is no change in expression of β1 integrin while the β7 integrin expression is decreased from 92% of eMMCs to 68% of MMCs. The majority of MMCs on D7 are positive for both αE and β7 integrin chains. Most of the CMCs in the trachea express β1 integrin and about one half are positive for β7 while only about one third express α4 and very few are positive for the αE integrin chains (data not shown).

Bottom Line: The resident tracheal CMCs had higher SSC, FcɛRI, and Kit and lower β7-integrin expression than the MMCs.By histology, the MMCs follow similar kinetics to the flow cytometry-identified mature MMCs and are notably persistent for >42 days.Steroid treatment reduced inflammation and MCp influx but had no effect on established MMCs.

View Article: PubMed Central - PubMed

Affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.

ABSTRACT
In contrast to resident constitutive mast cells (CMCs), mucosal MCs (MMCs) appear in the lung and trachea of sensitized mice only following inhalation challenge. We monitored the influx and maturation of MCs by their expression of Kit, FcɛRI, β7-integrin and side scatter (SSC) by flow cytometry. Influx of MC progenitors (MCps) (FcɛRI(lo), Kit(int), β7(hi), and SSC(lo)) peaks 1 day after challenges and subsides to baseline by day 7 after challenge. The mature MMCs appear as a distinct population on day 7 and peak at day 14 with higher SSC and FcɛRI expression, but lower β7 and Kit expression. A distinct transitional population is present between 1 and 7 days after challenge. Maturation occurs more rapidly in the trachea. The resident tracheal CMCs had higher SSC, FcɛRI, and Kit and lower β7-integrin expression than the MMCs. By histology, the MMCs follow similar kinetics to the flow cytometry-identified mature MMCs and are notably persistent for >42 days. Steroid treatment reduced inflammation and MCp influx but had no effect on established MMCs. Thus, changes in SSC, FcɛRI, and Kit together with the expression of αE/α4:β7-integrins characterizes the development of induced MMCs from MCps and distinguishes them from resident CMCs in the trachea and large airways.

No MeSH data available.


Related in: MedlinePlus