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The ß-importin KAP8 (Pse1/Kap121) is required for nuclear import of the cellulase transcriptional regulator XYR1, asexual sporulation and stress resistance in Trichoderma reesei.

Ghassemi S, Lichius A, Bidard F, Lemoine S, Rossignol MN, Herold S, Seidl-Seiboth V, Seiboth B, Espeso EA, Margeot A, Kubicek CP - Mol. Microbiol. (2015)

Bottom Line: We found KAP8, an ortholog of Aspergillus nidulans KapI, and Saccharomyces cerevisiae Kap121/Pse1, to be essential for nuclear recruitment of GFP-XYR1 and cellulase gene expression.Δkap8 strains were capable of forming fertile fruiting bodies but exhibited strongly reduced conidiation both in light and darkness, and showed enhanced sensitivity towards abiotic stress, including high osmotic pressure, low pH and high temperature.Together, these data underscore the significance of nuclear import of XYR1 in cellulase and hemicellulase gene regulation in T. reesei, and identify KAP8 as the major karyopherin required for this process.

View Article: PubMed Central - PubMed

Affiliation: Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, TU Wien, Vienna, 1060, Austria.

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Inventory of gene groups that were not induced by sophorose but significantly down-regulated (at least > 5-fold; P < 0.05) in the Δkap8 strain compared with the retransformant. The gene groups encounter 152 from 163 down-regulated genes (for full description, see Supporting Information Table S6). Specification of gene groups is as described in the legend to Fig. 3. New groups include HYDROLASES, extracellular lipases, esterases and amidases; SIGNAL TRANSDUCTION, involved in signal transduction pathways; and CELL WALL, proteins being components of the fungal cell wall, glycosyltransferases involved in their biosynthesis.
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Figure 7: Inventory of gene groups that were not induced by sophorose but significantly down-regulated (at least > 5-fold; P < 0.05) in the Δkap8 strain compared with the retransformant. The gene groups encounter 152 from 163 down-regulated genes (for full description, see Supporting Information Table S6). Specification of gene groups is as described in the legend to Fig. 3. New groups include HYDROLASES, extracellular lipases, esterases and amidases; SIGNAL TRANSDUCTION, involved in signal transduction pathways; and CELL WALL, proteins being components of the fungal cell wall, glycosyltransferases involved in their biosynthesis.

Mentions: To potentially identify genes that could be causally related to this reduced stress resistance, we searched our transcriptomic data for genes whose expression was KAP8 dependent but not linked to cellulose induction. Thus, we looked for genes whose expression on glycerol was either similar or even higher as that on sophorose but strongly reduced (> 5-fold, P < 0.05) under both conditions in the Δkap8 strain. One hundred sixty-three genes fulfilled this criterion (Supporting Information Table S6). Some of the major gene groups in this sample were the same as during induction with sophorose (i.e. genes encoding metabolic enzymes, MFS permeases and unknown proteins; Fig. 7). Among the other genes, however, a few examples are noteworthy, e.g. the two-component histidine kinase Trire2:70943; the aquaglyceroporin Trir2:81082; three PTH11-type G-protein coupled receptors Trire2: 82041, 69904 and 122795; the translation initiation regulator GCN20 (Trire2: 22839); several putative cell wall proteins (Trire2: 123659,123475; the Trichoderma cell wall protein QI74 Trire2:74282 (Rey et al., 1998); and five Zn(2)Cys(6) transcriptional activators of which the expression of two (Trire2: 112036, 112560) was absolutely dependent on KAP8 function.


The ß-importin KAP8 (Pse1/Kap121) is required for nuclear import of the cellulase transcriptional regulator XYR1, asexual sporulation and stress resistance in Trichoderma reesei.

Ghassemi S, Lichius A, Bidard F, Lemoine S, Rossignol MN, Herold S, Seidl-Seiboth V, Seiboth B, Espeso EA, Margeot A, Kubicek CP - Mol. Microbiol. (2015)

Inventory of gene groups that were not induced by sophorose but significantly down-regulated (at least > 5-fold; P < 0.05) in the Δkap8 strain compared with the retransformant. The gene groups encounter 152 from 163 down-regulated genes (for full description, see Supporting Information Table S6). Specification of gene groups is as described in the legend to Fig. 3. New groups include HYDROLASES, extracellular lipases, esterases and amidases; SIGNAL TRANSDUCTION, involved in signal transduction pathways; and CELL WALL, proteins being components of the fungal cell wall, glycosyltransferases involved in their biosynthesis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390390&req=5

Figure 7: Inventory of gene groups that were not induced by sophorose but significantly down-regulated (at least > 5-fold; P < 0.05) in the Δkap8 strain compared with the retransformant. The gene groups encounter 152 from 163 down-regulated genes (for full description, see Supporting Information Table S6). Specification of gene groups is as described in the legend to Fig. 3. New groups include HYDROLASES, extracellular lipases, esterases and amidases; SIGNAL TRANSDUCTION, involved in signal transduction pathways; and CELL WALL, proteins being components of the fungal cell wall, glycosyltransferases involved in their biosynthesis.
Mentions: To potentially identify genes that could be causally related to this reduced stress resistance, we searched our transcriptomic data for genes whose expression was KAP8 dependent but not linked to cellulose induction. Thus, we looked for genes whose expression on glycerol was either similar or even higher as that on sophorose but strongly reduced (> 5-fold, P < 0.05) under both conditions in the Δkap8 strain. One hundred sixty-three genes fulfilled this criterion (Supporting Information Table S6). Some of the major gene groups in this sample were the same as during induction with sophorose (i.e. genes encoding metabolic enzymes, MFS permeases and unknown proteins; Fig. 7). Among the other genes, however, a few examples are noteworthy, e.g. the two-component histidine kinase Trire2:70943; the aquaglyceroporin Trir2:81082; three PTH11-type G-protein coupled receptors Trire2: 82041, 69904 and 122795; the translation initiation regulator GCN20 (Trire2: 22839); several putative cell wall proteins (Trire2: 123659,123475; the Trichoderma cell wall protein QI74 Trire2:74282 (Rey et al., 1998); and five Zn(2)Cys(6) transcriptional activators of which the expression of two (Trire2: 112036, 112560) was absolutely dependent on KAP8 function.

Bottom Line: We found KAP8, an ortholog of Aspergillus nidulans KapI, and Saccharomyces cerevisiae Kap121/Pse1, to be essential for nuclear recruitment of GFP-XYR1 and cellulase gene expression.Δkap8 strains were capable of forming fertile fruiting bodies but exhibited strongly reduced conidiation both in light and darkness, and showed enhanced sensitivity towards abiotic stress, including high osmotic pressure, low pH and high temperature.Together, these data underscore the significance of nuclear import of XYR1 in cellulase and hemicellulase gene regulation in T. reesei, and identify KAP8 as the major karyopherin required for this process.

View Article: PubMed Central - PubMed

Affiliation: Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, TU Wien, Vienna, 1060, Austria.

Show MeSH
Related in: MedlinePlus