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Expanding the species and chemical diversity of Penicillium section Cinnamopurpurea.

Peterson SW, Jurjević Ž, Frisvad JC - PLoS ONE (2015)

Bottom Line: Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species.Seven new species were revealed in the analysis and are described here.These isolates could provide leads in pharmaceutical research.

View Article: PubMed Central - PubMed

Affiliation: Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois, 61604, United States of America.

ABSTRACT
A set of isolates very similar to or potentially conspecific with an unidentified Penicillium isolate NRRL 735, was assembled using a BLAST search of ITS similarity among described (GenBank) and undescribed Penicillium isolates in our laboratories. DNA was amplified from six loci of the assembled isolates and sequenced. Two species in section Cinnamopurpurea are self-compatible sexual species, but the asexual species had polymorphic loci suggestive of sexual reproduction and variation in conidium size suggestive of ploidy level differences typical of heterothallism. Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species. Seven new species were revealed in the analysis and are described here. Extrolite analysis showed that two of the new species, P. colei and P. monsserratidens produce the mycotoxin citreoviridin that has demonstrated pharmacological activity against human lung tumors. These isolates could provide leads in pharmaceutical research.

No MeSH data available.


Related in: MedlinePlus

Haplotype analysis among isolates of section Cinnamopurpurea at seven loci grouped by high bootstrap values listed in the last column.At each locus unique haplotypes are given arbitrary letter designations. Subgroups with strong statistical support (Fig. 1) are shaded in a distinct color for the multi-isolate species. Haplotypes shared between distinct clades of a species are presented with red lettering.
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pone.0121987.g003: Haplotype analysis among isolates of section Cinnamopurpurea at seven loci grouped by high bootstrap values listed in the last column.At each locus unique haplotypes are given arbitrary letter designations. Subgroups with strong statistical support (Fig. 1) are shaded in a distinct color for the multi-isolate species. Haplotypes shared between distinct clades of a species are presented with red lettering.

Mentions: The haplotypes of each isolate at each locus were compared and arbitrarily assigned letter designations (Fig. 3). In the figure, shading is used to represent groups of isolates with >90% bootstrap support in the multilocus tree (Fig. 1). Haplotypes are shared at some loci between subclades of species, but there are no shared haplotypes between species. The homothallic sexually reproducing species P. cinnamopurpureum has a pattern of haplotypes among the isolates similar to the patterns of the putatively clonal species P. cvjetkovicii and P. fluviserpens. Sharing haplotypes between strongly supported groups could result from incomplete lineage sorting [43] or from hyphal fusion of different genotypes in a homothallic species. Other species have only one or two representatives and patterns cannot be discerned.


Expanding the species and chemical diversity of Penicillium section Cinnamopurpurea.

Peterson SW, Jurjević Ž, Frisvad JC - PLoS ONE (2015)

Haplotype analysis among isolates of section Cinnamopurpurea at seven loci grouped by high bootstrap values listed in the last column.At each locus unique haplotypes are given arbitrary letter designations. Subgroups with strong statistical support (Fig. 1) are shaded in a distinct color for the multi-isolate species. Haplotypes shared between distinct clades of a species are presented with red lettering.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390383&req=5

pone.0121987.g003: Haplotype analysis among isolates of section Cinnamopurpurea at seven loci grouped by high bootstrap values listed in the last column.At each locus unique haplotypes are given arbitrary letter designations. Subgroups with strong statistical support (Fig. 1) are shaded in a distinct color for the multi-isolate species. Haplotypes shared between distinct clades of a species are presented with red lettering.
Mentions: The haplotypes of each isolate at each locus were compared and arbitrarily assigned letter designations (Fig. 3). In the figure, shading is used to represent groups of isolates with >90% bootstrap support in the multilocus tree (Fig. 1). Haplotypes are shared at some loci between subclades of species, but there are no shared haplotypes between species. The homothallic sexually reproducing species P. cinnamopurpureum has a pattern of haplotypes among the isolates similar to the patterns of the putatively clonal species P. cvjetkovicii and P. fluviserpens. Sharing haplotypes between strongly supported groups could result from incomplete lineage sorting [43] or from hyphal fusion of different genotypes in a homothallic species. Other species have only one or two representatives and patterns cannot be discerned.

Bottom Line: Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species.Seven new species were revealed in the analysis and are described here.These isolates could provide leads in pharmaceutical research.

View Article: PubMed Central - PubMed

Affiliation: Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois, 61604, United States of America.

ABSTRACT
A set of isolates very similar to or potentially conspecific with an unidentified Penicillium isolate NRRL 735, was assembled using a BLAST search of ITS similarity among described (GenBank) and undescribed Penicillium isolates in our laboratories. DNA was amplified from six loci of the assembled isolates and sequenced. Two species in section Cinnamopurpurea are self-compatible sexual species, but the asexual species had polymorphic loci suggestive of sexual reproduction and variation in conidium size suggestive of ploidy level differences typical of heterothallism. Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species. Seven new species were revealed in the analysis and are described here. Extrolite analysis showed that two of the new species, P. colei and P. monsserratidens produce the mycotoxin citreoviridin that has demonstrated pharmacological activity against human lung tumors. These isolates could provide leads in pharmaceutical research.

No MeSH data available.


Related in: MedlinePlus