Limits...
Expanding the species and chemical diversity of Penicillium section Cinnamopurpurea.

Peterson SW, Jurjević Ž, Frisvad JC - PLoS ONE (2015)

Bottom Line: Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species.Seven new species were revealed in the analysis and are described here.These isolates could provide leads in pharmaceutical research.

View Article: PubMed Central - PubMed

Affiliation: Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois, 61604, United States of America.

ABSTRACT
A set of isolates very similar to or potentially conspecific with an unidentified Penicillium isolate NRRL 735, was assembled using a BLAST search of ITS similarity among described (GenBank) and undescribed Penicillium isolates in our laboratories. DNA was amplified from six loci of the assembled isolates and sequenced. Two species in section Cinnamopurpurea are self-compatible sexual species, but the asexual species had polymorphic loci suggestive of sexual reproduction and variation in conidium size suggestive of ploidy level differences typical of heterothallism. Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species. Seven new species were revealed in the analysis and are described here. Extrolite analysis showed that two of the new species, P. colei and P. monsserratidens produce the mycotoxin citreoviridin that has demonstrated pharmacological activity against human lung tumors. These isolates could provide leads in pharmaceutical research.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of section Cinnamopurpurea.Fully resolved phylogenetic tree based on combined sequence data from BT2, CF, Mcm7, RPB2 and Tsr1, computed with maximum likelihood in Mega. In the bootstrap, all nodes were above 95%, except the P. fluviserpens subclade NRRL 35844, NRRL 58649 77%; P. cinnamopurpureum subclade NRRL 35501-NRRL 35502 55%; and P. cinnamopurpureum subclade NRRL 162-NRRL 3118-NRRL 3326-NRRL 35503 64%). Outgroup chosen based on prior work [27]. P. ellipsoideosporum is represented only by sequences obtained from GenBank.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4390383&req=5

pone.0121987.g001: Phylogenetic tree of section Cinnamopurpurea.Fully resolved phylogenetic tree based on combined sequence data from BT2, CF, Mcm7, RPB2 and Tsr1, computed with maximum likelihood in Mega. In the bootstrap, all nodes were above 95%, except the P. fluviserpens subclade NRRL 35844, NRRL 58649 77%; P. cinnamopurpureum subclade NRRL 35501-NRRL 35502 55%; and P. cinnamopurpureum subclade NRRL 162-NRRL 3118-NRRL 3326-NRRL 35503 64%). Outgroup chosen based on prior work [27]. P. ellipsoideosporum is represented only by sequences obtained from GenBank.

Mentions: The datasets examined contained, by locus, BT2, 489 aligned coding and non-coding characters, best model K2+G; CF, 741 aligned coding and non-coding characters, best model K2+G; ITS, 533 aligned characters from ITS1, ITS2 and 5.8S rDNA, best model JC+I; Mcm7, 616 aligned coding characters, best model K2+I; RPB2, 1014 aligned coding characters best model K2+G; and Tsr1, 816 coding characters, best model T92+G. In each of the single locus trees (S1 Fig.) isolates formed terminal groups with strong statistical support. Penicillium cvjetkovicii, P. lemhiflumine and P. fluviserpens are strongly supported at 6 of 6 loci, P. monsgalena, P. monsserratidens and P. idahoense are supported by 5 of the 6 loci, and P. colei and P. salmoniflumine are supported by 4 of the 6 loci. Some species and terminal groups had very low bootstrap support at the ITS locus, but the ITS region is an effective barcode for these species, with the five protein coding loci also useful for genotypic species recognition. Mcm7 and RPB2 loci display the lowest intraspecific variation and would be most suitable for diagnostic purposes. The datasets were concatenated and analyzed providing a fully resolved view of the group (Fig. 1).


Expanding the species and chemical diversity of Penicillium section Cinnamopurpurea.

Peterson SW, Jurjević Ž, Frisvad JC - PLoS ONE (2015)

Phylogenetic tree of section Cinnamopurpurea.Fully resolved phylogenetic tree based on combined sequence data from BT2, CF, Mcm7, RPB2 and Tsr1, computed with maximum likelihood in Mega. In the bootstrap, all nodes were above 95%, except the P. fluviserpens subclade NRRL 35844, NRRL 58649 77%; P. cinnamopurpureum subclade NRRL 35501-NRRL 35502 55%; and P. cinnamopurpureum subclade NRRL 162-NRRL 3118-NRRL 3326-NRRL 35503 64%). Outgroup chosen based on prior work [27]. P. ellipsoideosporum is represented only by sequences obtained from GenBank.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390383&req=5

pone.0121987.g001: Phylogenetic tree of section Cinnamopurpurea.Fully resolved phylogenetic tree based on combined sequence data from BT2, CF, Mcm7, RPB2 and Tsr1, computed with maximum likelihood in Mega. In the bootstrap, all nodes were above 95%, except the P. fluviserpens subclade NRRL 35844, NRRL 58649 77%; P. cinnamopurpureum subclade NRRL 35501-NRRL 35502 55%; and P. cinnamopurpureum subclade NRRL 162-NRRL 3118-NRRL 3326-NRRL 35503 64%). Outgroup chosen based on prior work [27]. P. ellipsoideosporum is represented only by sequences obtained from GenBank.
Mentions: The datasets examined contained, by locus, BT2, 489 aligned coding and non-coding characters, best model K2+G; CF, 741 aligned coding and non-coding characters, best model K2+G; ITS, 533 aligned characters from ITS1, ITS2 and 5.8S rDNA, best model JC+I; Mcm7, 616 aligned coding characters, best model K2+I; RPB2, 1014 aligned coding characters best model K2+G; and Tsr1, 816 coding characters, best model T92+G. In each of the single locus trees (S1 Fig.) isolates formed terminal groups with strong statistical support. Penicillium cvjetkovicii, P. lemhiflumine and P. fluviserpens are strongly supported at 6 of 6 loci, P. monsgalena, P. monsserratidens and P. idahoense are supported by 5 of the 6 loci, and P. colei and P. salmoniflumine are supported by 4 of the 6 loci. Some species and terminal groups had very low bootstrap support at the ITS locus, but the ITS region is an effective barcode for these species, with the five protein coding loci also useful for genotypic species recognition. Mcm7 and RPB2 loci display the lowest intraspecific variation and would be most suitable for diagnostic purposes. The datasets were concatenated and analyzed providing a fully resolved view of the group (Fig. 1).

Bottom Line: Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species.Seven new species were revealed in the analysis and are described here.These isolates could provide leads in pharmaceutical research.

View Article: PubMed Central - PubMed

Affiliation: Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois, 61604, United States of America.

ABSTRACT
A set of isolates very similar to or potentially conspecific with an unidentified Penicillium isolate NRRL 735, was assembled using a BLAST search of ITS similarity among described (GenBank) and undescribed Penicillium isolates in our laboratories. DNA was amplified from six loci of the assembled isolates and sequenced. Two species in section Cinnamopurpurea are self-compatible sexual species, but the asexual species had polymorphic loci suggestive of sexual reproduction and variation in conidium size suggestive of ploidy level differences typical of heterothallism. Accordingly we use genealogical concordance analysis, a technique valid only in heterothallic organisms, for putatively asexual species. Seven new species were revealed in the analysis and are described here. Extrolite analysis showed that two of the new species, P. colei and P. monsserratidens produce the mycotoxin citreoviridin that has demonstrated pharmacological activity against human lung tumors. These isolates could provide leads in pharmaceutical research.

No MeSH data available.


Related in: MedlinePlus