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Identification of novel adipokines in the joint. Differential expression in healthy and osteoarthritis tissues.

Conde J, Scotece M, Abella V, Gómez R, López V, Villar R, Hermida M, Pino J, Gómez-Reino JJ, Gualillo O - PLoS ONE (2015)

Bottom Line: Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples.Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

View Article: PubMed Central - PubMed

Affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), the NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Research Laboratory 9, Santiago University Clinical Hospital, Santiago de Compostela, Spain.

ABSTRACT

Objectives: Emerging data suggest that several metabolic factors, released mainly by white adipose tissue (WAT) and joint tissues, and collectively named adipokines, might have a role in the pathophysiology of OA. Recently, novel adipokines such as SERPINE2, WISP2, GPNMB and ITIH5 have been identified in WAT. The main goal of this study was to analyse the expression of these novel adipokines in synovium, infrapatellar fat pad and chondrocytes and to compare the expression of these molecules in healthy and OA tissues.

Methods: Synovial tissues, infrapatellar fat pad and chondrocytes were obtained from 36 OA patients (age 52-85; mean BMI 28.9) who underwent total knee replacement surgery. Healthy synovial tissues and infrapatellar fat pad were obtained from 15 traumatic knee patients (age 23-53; mean BMI 23.5). mRNA and protein expression were determined by qRT-PCR and western blot analysis respectively.

Results: All the novel adipokines, matter of our study, are expressed in OA synovium, infrapatellar fat pad and chondrocytes. Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples. Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.

Conclusions: In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

No MeSH data available.


Related in: MedlinePlus

Determination of SERPINE2, WISP2 and ITIH5 mRNA and protein expression by quantitative real-time PCR and western blot.A. PCR results were shown in fold change, where black bars represent the mRNA expression of SERPINE2 and ITIH5 in 10 different synovial tissues obtained from healthy donors. Grey bars represent the mRNA expression of SERPINE2 and ITIH5 in 30 different synovial tissues obtained from OA patients. B. PCR results were shown in fold change, where black bars represent the mRNA expression of WISP2 in IPFP obtained from 10 healthy donors. Grey bars represent the mRNA expression of WISP2 in IPFP obtained from 30 OA patients. Statistical analysis was performed using unpaired t-test. P value * = p<0.05; ** = p<0.01 C vs OA.
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pone.0123601.g002: Determination of SERPINE2, WISP2 and ITIH5 mRNA and protein expression by quantitative real-time PCR and western blot.A. PCR results were shown in fold change, where black bars represent the mRNA expression of SERPINE2 and ITIH5 in 10 different synovial tissues obtained from healthy donors. Grey bars represent the mRNA expression of SERPINE2 and ITIH5 in 30 different synovial tissues obtained from OA patients. B. PCR results were shown in fold change, where black bars represent the mRNA expression of WISP2 in IPFP obtained from 10 healthy donors. Grey bars represent the mRNA expression of WISP2 in IPFP obtained from 30 OA patients. Statistical analysis was performed using unpaired t-test. P value * = p<0.05; ** = p<0.01 C vs OA.

Mentions: Comparison between healthy and OA synovium. As shown in Fig 2A, SERPINE2 mRNA and protein expression was significantly up-regulated in OA synovial tissues in comparison to healthy samples. On the other hand, ITIH5 expression was decreased in OA tissues (Fig 2A). However, we did not observe any variation on WISP2 or GPNMB expression between OA and healthy synovial tissues (data not shown).


Identification of novel adipokines in the joint. Differential expression in healthy and osteoarthritis tissues.

Conde J, Scotece M, Abella V, Gómez R, López V, Villar R, Hermida M, Pino J, Gómez-Reino JJ, Gualillo O - PLoS ONE (2015)

Determination of SERPINE2, WISP2 and ITIH5 mRNA and protein expression by quantitative real-time PCR and western blot.A. PCR results were shown in fold change, where black bars represent the mRNA expression of SERPINE2 and ITIH5 in 10 different synovial tissues obtained from healthy donors. Grey bars represent the mRNA expression of SERPINE2 and ITIH5 in 30 different synovial tissues obtained from OA patients. B. PCR results were shown in fold change, where black bars represent the mRNA expression of WISP2 in IPFP obtained from 10 healthy donors. Grey bars represent the mRNA expression of WISP2 in IPFP obtained from 30 OA patients. Statistical analysis was performed using unpaired t-test. P value * = p<0.05; ** = p<0.01 C vs OA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390373&req=5

pone.0123601.g002: Determination of SERPINE2, WISP2 and ITIH5 mRNA and protein expression by quantitative real-time PCR and western blot.A. PCR results were shown in fold change, where black bars represent the mRNA expression of SERPINE2 and ITIH5 in 10 different synovial tissues obtained from healthy donors. Grey bars represent the mRNA expression of SERPINE2 and ITIH5 in 30 different synovial tissues obtained from OA patients. B. PCR results were shown in fold change, where black bars represent the mRNA expression of WISP2 in IPFP obtained from 10 healthy donors. Grey bars represent the mRNA expression of WISP2 in IPFP obtained from 30 OA patients. Statistical analysis was performed using unpaired t-test. P value * = p<0.05; ** = p<0.01 C vs OA.
Mentions: Comparison between healthy and OA synovium. As shown in Fig 2A, SERPINE2 mRNA and protein expression was significantly up-regulated in OA synovial tissues in comparison to healthy samples. On the other hand, ITIH5 expression was decreased in OA tissues (Fig 2A). However, we did not observe any variation on WISP2 or GPNMB expression between OA and healthy synovial tissues (data not shown).

Bottom Line: Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples.Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

View Article: PubMed Central - PubMed

Affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), the NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Research Laboratory 9, Santiago University Clinical Hospital, Santiago de Compostela, Spain.

ABSTRACT

Objectives: Emerging data suggest that several metabolic factors, released mainly by white adipose tissue (WAT) and joint tissues, and collectively named adipokines, might have a role in the pathophysiology of OA. Recently, novel adipokines such as SERPINE2, WISP2, GPNMB and ITIH5 have been identified in WAT. The main goal of this study was to analyse the expression of these novel adipokines in synovium, infrapatellar fat pad and chondrocytes and to compare the expression of these molecules in healthy and OA tissues.

Methods: Synovial tissues, infrapatellar fat pad and chondrocytes were obtained from 36 OA patients (age 52-85; mean BMI 28.9) who underwent total knee replacement surgery. Healthy synovial tissues and infrapatellar fat pad were obtained from 15 traumatic knee patients (age 23-53; mean BMI 23.5). mRNA and protein expression were determined by qRT-PCR and western blot analysis respectively.

Results: All the novel adipokines, matter of our study, are expressed in OA synovium, infrapatellar fat pad and chondrocytes. Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples. Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.

Conclusions: In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

No MeSH data available.


Related in: MedlinePlus