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Identification of novel adipokines in the joint. Differential expression in healthy and osteoarthritis tissues.

Conde J, Scotece M, Abella V, Gómez R, López V, Villar R, Hermida M, Pino J, Gómez-Reino JJ, Gualillo O - PLoS ONE (2015)

Bottom Line: Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples.Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

View Article: PubMed Central - PubMed

Affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), the NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Research Laboratory 9, Santiago University Clinical Hospital, Santiago de Compostela, Spain.

ABSTRACT

Objectives: Emerging data suggest that several metabolic factors, released mainly by white adipose tissue (WAT) and joint tissues, and collectively named adipokines, might have a role in the pathophysiology of OA. Recently, novel adipokines such as SERPINE2, WISP2, GPNMB and ITIH5 have been identified in WAT. The main goal of this study was to analyse the expression of these novel adipokines in synovium, infrapatellar fat pad and chondrocytes and to compare the expression of these molecules in healthy and OA tissues.

Methods: Synovial tissues, infrapatellar fat pad and chondrocytes were obtained from 36 OA patients (age 52-85; mean BMI 28.9) who underwent total knee replacement surgery. Healthy synovial tissues and infrapatellar fat pad were obtained from 15 traumatic knee patients (age 23-53; mean BMI 23.5). mRNA and protein expression were determined by qRT-PCR and western blot analysis respectively.

Results: All the novel adipokines, matter of our study, are expressed in OA synovium, infrapatellar fat pad and chondrocytes. Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples. Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.

Conclusions: In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

No MeSH data available.


Related in: MedlinePlus

SERPINE2, WISP2, GPNMB and ITIH5 expression evaluated by real-time reverse transcription PCR (qRT-PCR) in synovia, IPFP and chondrocytes obtained from OA patients.A. Amplicons were electrophoresed on 2% agarose gel and stained with ethidium bromide. B. Determination of the basal mRNA expression of SERPINE2, WISP2, GPNMB and ITIH5 in synovial tissues, IPFP and chondrocytes of 30 OA patients. The results were expressed using the ΔΔCt data analysis method. Statistical analysis was performed using One-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P value *** = p<0.001 WISP2 vs SERPINE2; GPNMN; ITIH5.
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pone.0123601.g001: SERPINE2, WISP2, GPNMB and ITIH5 expression evaluated by real-time reverse transcription PCR (qRT-PCR) in synovia, IPFP and chondrocytes obtained from OA patients.A. Amplicons were electrophoresed on 2% agarose gel and stained with ethidium bromide. B. Determination of the basal mRNA expression of SERPINE2, WISP2, GPNMB and ITIH5 in synovial tissues, IPFP and chondrocytes of 30 OA patients. The results were expressed using the ΔΔCt data analysis method. Statistical analysis was performed using One-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P value *** = p<0.001 WISP2 vs SERPINE2; GPNMN; ITIH5.

Mentions: Constitutive expression of SERPINE2, WISP2, GPNMB and ITIH5 in synovium, infrapatellar fat pad and chondrocytes from OA patients. As shown in Fig 1A, we detected the mRNA expression of all the adipokines in the OA synovial tissues and in the IPFPs. These genes displayed a similar expression pattern in both tissues, being WISP2 the most expressed adipokine (Fig 1A).


Identification of novel adipokines in the joint. Differential expression in healthy and osteoarthritis tissues.

Conde J, Scotece M, Abella V, Gómez R, López V, Villar R, Hermida M, Pino J, Gómez-Reino JJ, Gualillo O - PLoS ONE (2015)

SERPINE2, WISP2, GPNMB and ITIH5 expression evaluated by real-time reverse transcription PCR (qRT-PCR) in synovia, IPFP and chondrocytes obtained from OA patients.A. Amplicons were electrophoresed on 2% agarose gel and stained with ethidium bromide. B. Determination of the basal mRNA expression of SERPINE2, WISP2, GPNMB and ITIH5 in synovial tissues, IPFP and chondrocytes of 30 OA patients. The results were expressed using the ΔΔCt data analysis method. Statistical analysis was performed using One-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P value *** = p<0.001 WISP2 vs SERPINE2; GPNMN; ITIH5.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390373&req=5

pone.0123601.g001: SERPINE2, WISP2, GPNMB and ITIH5 expression evaluated by real-time reverse transcription PCR (qRT-PCR) in synovia, IPFP and chondrocytes obtained from OA patients.A. Amplicons were electrophoresed on 2% agarose gel and stained with ethidium bromide. B. Determination of the basal mRNA expression of SERPINE2, WISP2, GPNMB and ITIH5 in synovial tissues, IPFP and chondrocytes of 30 OA patients. The results were expressed using the ΔΔCt data analysis method. Statistical analysis was performed using One-way ANOVA followed by Newman-Keuls Multiple Comparison Test. P value *** = p<0.001 WISP2 vs SERPINE2; GPNMN; ITIH5.
Mentions: Constitutive expression of SERPINE2, WISP2, GPNMB and ITIH5 in synovium, infrapatellar fat pad and chondrocytes from OA patients. As shown in Fig 1A, we detected the mRNA expression of all the adipokines in the OA synovial tissues and in the IPFPs. These genes displayed a similar expression pattern in both tissues, being WISP2 the most expressed adipokine (Fig 1A).

Bottom Line: Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples.Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

View Article: PubMed Central - PubMed

Affiliation: SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), the NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases), Research Laboratory 9, Santiago University Clinical Hospital, Santiago de Compostela, Spain.

ABSTRACT

Objectives: Emerging data suggest that several metabolic factors, released mainly by white adipose tissue (WAT) and joint tissues, and collectively named adipokines, might have a role in the pathophysiology of OA. Recently, novel adipokines such as SERPINE2, WISP2, GPNMB and ITIH5 have been identified in WAT. The main goal of this study was to analyse the expression of these novel adipokines in synovium, infrapatellar fat pad and chondrocytes and to compare the expression of these molecules in healthy and OA tissues.

Methods: Synovial tissues, infrapatellar fat pad and chondrocytes were obtained from 36 OA patients (age 52-85; mean BMI 28.9) who underwent total knee replacement surgery. Healthy synovial tissues and infrapatellar fat pad were obtained from 15 traumatic knee patients (age 23-53; mean BMI 23.5). mRNA and protein expression were determined by qRT-PCR and western blot analysis respectively.

Results: All the novel adipokines, matter of our study, are expressed in OA synovium, infrapatellar fat pad and chondrocytes. Moreover, we detected a differential expression of SERPINE2 and ITIH5 in OA synovial tissues as compared to healthy samples. Finally, we also observed an increased expression of WISP2 in OA infrapatellar fat pad in comparison to healthy controls.

Conclusions: In this study we demonstrated for the first time the expression of four novel adipokines in different joint tissues and how these molecules are differentially expressed in healthy and OA joint tissues.

No MeSH data available.


Related in: MedlinePlus