Limits...
Effect of vitrification on the microRNA transcriptome in mouse blastocysts.

Zhao X, Hao H, Du W, Zhu H - PLoS ONE (2015)

Bottom Line: However, little information is available about the effect of vitrification on the miRNA transcriptome in blastocysts.Of these, the expression levels of five miRNAs differed significantly: in the vitrified blastocysts, four miRNAs (mmu-miR-199a-5p, mmu-miR-329-3p, mmu-miR-136-5p and mmu-miR-16-1-3p) were upregulated, and one (mmu-miR-212-3p) was downregulated.The biological analysis further showed that the differentially expressed miRNAs mainly regulated the implantation of embryos.

View Article: PubMed Central - PubMed

Affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, P. R. China.

ABSTRACT
Vitrification is commonly used in the cryopreservation of mammalian blastocysts to overcome the temporal and spatial limitations of embryo transfer. Previous studies have shown that the implantation ability of vitrified blastocysts is impaired and that microRNAs (miRNAs) regulate the critical genes for embryo implantation. However, little information is available about the effect of vitrification on the miRNA transcriptome in blastocysts. In the present study, the miRNA transcriptomes in fresh and vitrified mouse blastocysts were analyzed by miRNA Taqman assay based method, and the results were validated using quantitative real-time PCR (qRT-PCR). Then, the differentially expressed miRNAs were assessed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Overall, 760 known mouse miRNAs were detected in the vitrified and fresh mouse blastocysts. Of these, the expression levels of five miRNAs differed significantly: in the vitrified blastocysts, four miRNAs (mmu-miR-199a-5p, mmu-miR-329-3p, mmu-miR-136-5p and mmu-miR-16-1-3p) were upregulated, and one (mmu-miR-212-3p) was downregulated. The expression levels of all miRNAs measured by the miRNA Taqman assay based method and qRT-PCR were consistent. The four upregulated miRNAs were predicted to regulate 877 candidate target genes, and the downregulated miRNA was predicted to regulate 231 genes. The biological analysis further showed that the differentially expressed miRNAs mainly regulated the implantation of embryos. In conclusion, the results of our study showed that vitrification significantly altered the miRNA transcriptome in mouse blastocysts, which may decrease the implantation potential of vitrified blastocysts.

No MeSH data available.


Related in: MedlinePlus

Heat map of differentially expressed miRNAs in the vitrification and control groups.F1, F2, and F3 are the samples from the fresh blastocyst group. V1, V2, and V3 are the samples from the vitrified blastocyst group. Red indicates upregulated expression, and green indicates downregulated expression, with respect to a reference expression level.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4390370&req=5

pone.0123451.g001: Heat map of differentially expressed miRNAs in the vitrification and control groups.F1, F2, and F3 are the samples from the fresh blastocyst group. V1, V2, and V3 are the samples from the vitrified blastocyst group. Red indicates upregulated expression, and green indicates downregulated expression, with respect to a reference expression level.

Mentions: S1 Table presented the fold change of the miRNAs in vitrified blastocysts. Compared to the expression levels in the fresh blastocysts, five miRNAs showed significantly different expression in the vitrified blastocysts (Fig 1). Of these miRNAs, four were upregulated (mmu-miR-199a-5p, mmu-miR-329-3p, mmu-miR-136-5p, and mmu-miR-16-1-3p), and one was downregulated (mmu-miR-212-3p).


Effect of vitrification on the microRNA transcriptome in mouse blastocysts.

Zhao X, Hao H, Du W, Zhu H - PLoS ONE (2015)

Heat map of differentially expressed miRNAs in the vitrification and control groups.F1, F2, and F3 are the samples from the fresh blastocyst group. V1, V2, and V3 are the samples from the vitrified blastocyst group. Red indicates upregulated expression, and green indicates downregulated expression, with respect to a reference expression level.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390370&req=5

pone.0123451.g001: Heat map of differentially expressed miRNAs in the vitrification and control groups.F1, F2, and F3 are the samples from the fresh blastocyst group. V1, V2, and V3 are the samples from the vitrified blastocyst group. Red indicates upregulated expression, and green indicates downregulated expression, with respect to a reference expression level.
Mentions: S1 Table presented the fold change of the miRNAs in vitrified blastocysts. Compared to the expression levels in the fresh blastocysts, five miRNAs showed significantly different expression in the vitrified blastocysts (Fig 1). Of these miRNAs, four were upregulated (mmu-miR-199a-5p, mmu-miR-329-3p, mmu-miR-136-5p, and mmu-miR-16-1-3p), and one was downregulated (mmu-miR-212-3p).

Bottom Line: However, little information is available about the effect of vitrification on the miRNA transcriptome in blastocysts.Of these, the expression levels of five miRNAs differed significantly: in the vitrified blastocysts, four miRNAs (mmu-miR-199a-5p, mmu-miR-329-3p, mmu-miR-136-5p and mmu-miR-16-1-3p) were upregulated, and one (mmu-miR-212-3p) was downregulated.The biological analysis further showed that the differentially expressed miRNAs mainly regulated the implantation of embryos.

View Article: PubMed Central - PubMed

Affiliation: Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, P. R. China.

ABSTRACT
Vitrification is commonly used in the cryopreservation of mammalian blastocysts to overcome the temporal and spatial limitations of embryo transfer. Previous studies have shown that the implantation ability of vitrified blastocysts is impaired and that microRNAs (miRNAs) regulate the critical genes for embryo implantation. However, little information is available about the effect of vitrification on the miRNA transcriptome in blastocysts. In the present study, the miRNA transcriptomes in fresh and vitrified mouse blastocysts were analyzed by miRNA Taqman assay based method, and the results were validated using quantitative real-time PCR (qRT-PCR). Then, the differentially expressed miRNAs were assessed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Overall, 760 known mouse miRNAs were detected in the vitrified and fresh mouse blastocysts. Of these, the expression levels of five miRNAs differed significantly: in the vitrified blastocysts, four miRNAs (mmu-miR-199a-5p, mmu-miR-329-3p, mmu-miR-136-5p and mmu-miR-16-1-3p) were upregulated, and one (mmu-miR-212-3p) was downregulated. The expression levels of all miRNAs measured by the miRNA Taqman assay based method and qRT-PCR were consistent. The four upregulated miRNAs were predicted to regulate 877 candidate target genes, and the downregulated miRNA was predicted to regulate 231 genes. The biological analysis further showed that the differentially expressed miRNAs mainly regulated the implantation of embryos. In conclusion, the results of our study showed that vitrification significantly altered the miRNA transcriptome in mouse blastocysts, which may decrease the implantation potential of vitrified blastocysts.

No MeSH data available.


Related in: MedlinePlus