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Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens).

Price BW, Henry CS, Hall AC, Mochizuki A, Duelli P, Brooks SJ - PLoS ONE (2015)

Bottom Line: Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes.One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis.Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.

View Article: PubMed Central - PubMed

Affiliation: Life Sciences Department, Natural History Museum, London, England.

ABSTRACT
Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.

No MeSH data available.


Maximum Likelihood phylogram of the cryptic species of the Chrysoperla carnea-group.Phylogram is based on analysis of 1226 bp of COI sequence. Numbers at the branch points are bootstrap support (above) and Bayesian posterior probabilities (below); branch lengths are proportional to the number of substitutions per site except where indicated. Positions of the test and lectotype specimens in the phylogram are shown in red.
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pone.0121127.g003: Maximum Likelihood phylogram of the cryptic species of the Chrysoperla carnea-group.Phylogram is based on analysis of 1226 bp of COI sequence. Numbers at the branch points are bootstrap support (above) and Bayesian posterior probabilities (below); branch lengths are proportional to the number of substitutions per site except where indicated. Positions of the test and lectotype specimens in the phylogram are shown in red.

Mentions: Likelihood and Bayesian analysis of the COI data alone (Fig. 3) did not significantly affect the topology in comparison to the combined mitochondrial dataset; however, support values were reduced across the tree. Both the test and lectotype specimens fell within the C. carnea clade with 100% bootstrap and 0.77 posterior probability support. Data files (analysis and resulting tree files) available on the NHM data portal: http://dx.doi.org/10.5519/0059186.


Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens).

Price BW, Henry CS, Hall AC, Mochizuki A, Duelli P, Brooks SJ - PLoS ONE (2015)

Maximum Likelihood phylogram of the cryptic species of the Chrysoperla carnea-group.Phylogram is based on analysis of 1226 bp of COI sequence. Numbers at the branch points are bootstrap support (above) and Bayesian posterior probabilities (below); branch lengths are proportional to the number of substitutions per site except where indicated. Positions of the test and lectotype specimens in the phylogram are shown in red.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390323&req=5

pone.0121127.g003: Maximum Likelihood phylogram of the cryptic species of the Chrysoperla carnea-group.Phylogram is based on analysis of 1226 bp of COI sequence. Numbers at the branch points are bootstrap support (above) and Bayesian posterior probabilities (below); branch lengths are proportional to the number of substitutions per site except where indicated. Positions of the test and lectotype specimens in the phylogram are shown in red.
Mentions: Likelihood and Bayesian analysis of the COI data alone (Fig. 3) did not significantly affect the topology in comparison to the combined mitochondrial dataset; however, support values were reduced across the tree. Both the test and lectotype specimens fell within the C. carnea clade with 100% bootstrap and 0.77 posterior probability support. Data files (analysis and resulting tree files) available on the NHM data portal: http://dx.doi.org/10.5519/0059186.

Bottom Line: Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes.One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis.Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.

View Article: PubMed Central - PubMed

Affiliation: Life Sciences Department, Natural History Museum, London, England.

ABSTRACT
Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.

No MeSH data available.