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Investigation of genes encoding calcineurin B-like protein family in legumes and their expression analyses in chickpea (Cicer arietinum L.).

Meena MK, Ghawana S, Sardar A, Dwivedi V, Khandal H, Roy R, Chattopadhyay D - PLoS ONE (2015)

Bottom Line: Most of the CaCBL genes exhibited high expression in flowers.Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance.These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

ABSTRACT
Calcium ion (Ca2+) is a ubiquitous second messenger that transmits various internal and external signals including stresses and, therefore, is important for plants' response process. Calcineurin B-like proteins (CBLs) are one of the plant calcium sensors, which sense and convey the changes in cytosolic Ca2+-concentration for response process. A search in four leguminous plant (soybean, Medicago truncatula, common bean and chickpea) genomes identified 9 to 15 genes in each species that encode CBL proteins. Sequence analyses of CBL peptides and coding sequences (CDS) suggested that there are nine original CBL genes in these legumes and some of them were multiplied during whole genome or local gene duplication. Coding sequences of chickpea CBL genes (CaCBL) were cloned from their cDNAs and sequenced, and their annotations in the genome assemblies were corrected accordingly. Analyses of protein sequences and gene structures of CBL family in plant kingdom indicated its diverse origin but showed a remarkable conservation in overall protein structure with appearance of complex gene structure in the course of evolution. Expression of CaCBL genes in different tissues and in response to different stress and hormone treatment were studied. Most of the CaCBL genes exhibited high expression in flowers. Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance. These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

No MeSH data available.


Relative transcript levels of chickpea CBL genes in response to treatment with various phytohormones.Expression profiles of CBL genes in chickpea seedlings exposed to Abscisic acid (A), BAP (B), IAA (C), Salicylic acid (D) and Methyl Jasmonate (E) for different period by qRT-PCR and presented as heatmap. The scale bar represents relative expression value. Hierarchical clustering was played in data analysis. Relative fold expression values are presented as bar diagram in S4 Fig.
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pone.0123640.g006: Relative transcript levels of chickpea CBL genes in response to treatment with various phytohormones.Expression profiles of CBL genes in chickpea seedlings exposed to Abscisic acid (A), BAP (B), IAA (C), Salicylic acid (D) and Methyl Jasmonate (E) for different period by qRT-PCR and presented as heatmap. The scale bar represents relative expression value. Hierarchical clustering was played in data analysis. Relative fold expression values are presented as bar diagram in S4 Fig.

Mentions: Expressions of CaCBL4 and -8 are consistently decreased with time in response to ABA treatment. CaCBL10 expression did not alter by the treatment. Expressions of rest of the CaCBL genes were enhanced with exposure period, however, not steadily. The maximum fold increase was observed for CaCBL6 gene expression, which was enhanced by more than 6-folds after six hours of treatment (Fig 6A). CaCBL1, -2, -3 and -9 registered similar enhancement in expression. Expression patterns of CaCBL1 and -9 in response to ABA are in accordance with the role of their orthologs in Arabidopsis in ABA-mediated signaling.


Investigation of genes encoding calcineurin B-like protein family in legumes and their expression analyses in chickpea (Cicer arietinum L.).

Meena MK, Ghawana S, Sardar A, Dwivedi V, Khandal H, Roy R, Chattopadhyay D - PLoS ONE (2015)

Relative transcript levels of chickpea CBL genes in response to treatment with various phytohormones.Expression profiles of CBL genes in chickpea seedlings exposed to Abscisic acid (A), BAP (B), IAA (C), Salicylic acid (D) and Methyl Jasmonate (E) for different period by qRT-PCR and presented as heatmap. The scale bar represents relative expression value. Hierarchical clustering was played in data analysis. Relative fold expression values are presented as bar diagram in S4 Fig.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390317&req=5

pone.0123640.g006: Relative transcript levels of chickpea CBL genes in response to treatment with various phytohormones.Expression profiles of CBL genes in chickpea seedlings exposed to Abscisic acid (A), BAP (B), IAA (C), Salicylic acid (D) and Methyl Jasmonate (E) for different period by qRT-PCR and presented as heatmap. The scale bar represents relative expression value. Hierarchical clustering was played in data analysis. Relative fold expression values are presented as bar diagram in S4 Fig.
Mentions: Expressions of CaCBL4 and -8 are consistently decreased with time in response to ABA treatment. CaCBL10 expression did not alter by the treatment. Expressions of rest of the CaCBL genes were enhanced with exposure period, however, not steadily. The maximum fold increase was observed for CaCBL6 gene expression, which was enhanced by more than 6-folds after six hours of treatment (Fig 6A). CaCBL1, -2, -3 and -9 registered similar enhancement in expression. Expression patterns of CaCBL1 and -9 in response to ABA are in accordance with the role of their orthologs in Arabidopsis in ABA-mediated signaling.

Bottom Line: Most of the CaCBL genes exhibited high expression in flowers.Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance.These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

ABSTRACT
Calcium ion (Ca2+) is a ubiquitous second messenger that transmits various internal and external signals including stresses and, therefore, is important for plants' response process. Calcineurin B-like proteins (CBLs) are one of the plant calcium sensors, which sense and convey the changes in cytosolic Ca2+-concentration for response process. A search in four leguminous plant (soybean, Medicago truncatula, common bean and chickpea) genomes identified 9 to 15 genes in each species that encode CBL proteins. Sequence analyses of CBL peptides and coding sequences (CDS) suggested that there are nine original CBL genes in these legumes and some of them were multiplied during whole genome or local gene duplication. Coding sequences of chickpea CBL genes (CaCBL) were cloned from their cDNAs and sequenced, and their annotations in the genome assemblies were corrected accordingly. Analyses of protein sequences and gene structures of CBL family in plant kingdom indicated its diverse origin but showed a remarkable conservation in overall protein structure with appearance of complex gene structure in the course of evolution. Expression of CaCBL genes in different tissues and in response to different stress and hormone treatment were studied. Most of the CaCBL genes exhibited high expression in flowers. Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance. These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

No MeSH data available.