Limits...
Investigation of genes encoding calcineurin B-like protein family in legumes and their expression analyses in chickpea (Cicer arietinum L.).

Meena MK, Ghawana S, Sardar A, Dwivedi V, Khandal H, Roy R, Chattopadhyay D - PLoS ONE (2015)

Bottom Line: Most of the CaCBL genes exhibited high expression in flowers.Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance.These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

ABSTRACT
Calcium ion (Ca2+) is a ubiquitous second messenger that transmits various internal and external signals including stresses and, therefore, is important for plants' response process. Calcineurin B-like proteins (CBLs) are one of the plant calcium sensors, which sense and convey the changes in cytosolic Ca2+-concentration for response process. A search in four leguminous plant (soybean, Medicago truncatula, common bean and chickpea) genomes identified 9 to 15 genes in each species that encode CBL proteins. Sequence analyses of CBL peptides and coding sequences (CDS) suggested that there are nine original CBL genes in these legumes and some of them were multiplied during whole genome or local gene duplication. Coding sequences of chickpea CBL genes (CaCBL) were cloned from their cDNAs and sequenced, and their annotations in the genome assemblies were corrected accordingly. Analyses of protein sequences and gene structures of CBL family in plant kingdom indicated its diverse origin but showed a remarkable conservation in overall protein structure with appearance of complex gene structure in the course of evolution. Expression of CaCBL genes in different tissues and in response to different stress and hormone treatment were studied. Most of the CaCBL genes exhibited high expression in flowers. Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance. These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

No MeSH data available.


Relative transcript levels of chickpea CBL genes in response to different abiotic stress treatments.6 day-old chickpea seedlings were exposed to 250 mM NaCl (A), 20% PEG (B) and Cold at 4°C (C) and samples were harvested at different time interval as mentioned for relative expression studies by qRT-PCR and presented as heatmap. The scale bar represents relative expression values. Hierarchical clustering has been represented at left. Relative fold expression values are presented as bar diagram in S4 Fig.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4390317&req=5

pone.0123640.g005: Relative transcript levels of chickpea CBL genes in response to different abiotic stress treatments.6 day-old chickpea seedlings were exposed to 250 mM NaCl (A), 20% PEG (B) and Cold at 4°C (C) and samples were harvested at different time interval as mentioned for relative expression studies by qRT-PCR and presented as heatmap. The scale bar represents relative expression values. Hierarchical clustering has been represented at left. Relative fold expression values are presented as bar diagram in S4 Fig.

Mentions: The first CBL gene (AtCBL4/SOS3) was identified in the screening for salt-overly-sensitive phenotype. Subsequent reports suggest that most of the CBL and CIPK genes were found associated with signaling related to abiotic stress and abiotic stress-related hormones. To investigate the potential role of CaCBL genes in abiotic stress response, expression studies were performed under different conditions of stresses for different time periods. 6 day-old chickpea seedlings were treated with 250mM NaCl (salt) or 20% PEG (dehydration) or 4°C (cold). The relative expressions were converted to log 2 values of fold changes and presented as heatmap with reference to the value at control condition. Exposure to 250mM sodium chloride appears to have insignificant effect on expression of CaCBL5, -8 and -6 (Fig 5A). Surprisingly, expression of CaCBL4 was downregulated by salt exposure, which suggested that it functions differently from its ortholog in Arabidopsis. Expression of other CaCBLs was increased by salt treatment. Several studies have suggested that overlapping signaling pathways operate in response to drought and salinity. Apart from CaCBL5, expression of all other chickpea CBL genes under PEG treatment followed same pattern of their expression in response to salinity. Expressions of CaCBL6, -4 and -8 were either downregulated or unaltered under PEG treatment. In contrast to salt treatment, dehydration enhanced expression of CaCBL5 by ten fold. CaCBL10 registered the highest fold (16-fold) of expression under salinity, while its expression under dehydration was periodically upregulated only at 1hr and 12hr. CaCBL1 registered the highest fold (17-fold) of expression under dehydration at 24hr (Fig 5B).


Investigation of genes encoding calcineurin B-like protein family in legumes and their expression analyses in chickpea (Cicer arietinum L.).

Meena MK, Ghawana S, Sardar A, Dwivedi V, Khandal H, Roy R, Chattopadhyay D - PLoS ONE (2015)

Relative transcript levels of chickpea CBL genes in response to different abiotic stress treatments.6 day-old chickpea seedlings were exposed to 250 mM NaCl (A), 20% PEG (B) and Cold at 4°C (C) and samples were harvested at different time interval as mentioned for relative expression studies by qRT-PCR and presented as heatmap. The scale bar represents relative expression values. Hierarchical clustering has been represented at left. Relative fold expression values are presented as bar diagram in S4 Fig.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390317&req=5

pone.0123640.g005: Relative transcript levels of chickpea CBL genes in response to different abiotic stress treatments.6 day-old chickpea seedlings were exposed to 250 mM NaCl (A), 20% PEG (B) and Cold at 4°C (C) and samples were harvested at different time interval as mentioned for relative expression studies by qRT-PCR and presented as heatmap. The scale bar represents relative expression values. Hierarchical clustering has been represented at left. Relative fold expression values are presented as bar diagram in S4 Fig.
Mentions: The first CBL gene (AtCBL4/SOS3) was identified in the screening for salt-overly-sensitive phenotype. Subsequent reports suggest that most of the CBL and CIPK genes were found associated with signaling related to abiotic stress and abiotic stress-related hormones. To investigate the potential role of CaCBL genes in abiotic stress response, expression studies were performed under different conditions of stresses for different time periods. 6 day-old chickpea seedlings were treated with 250mM NaCl (salt) or 20% PEG (dehydration) or 4°C (cold). The relative expressions were converted to log 2 values of fold changes and presented as heatmap with reference to the value at control condition. Exposure to 250mM sodium chloride appears to have insignificant effect on expression of CaCBL5, -8 and -6 (Fig 5A). Surprisingly, expression of CaCBL4 was downregulated by salt exposure, which suggested that it functions differently from its ortholog in Arabidopsis. Expression of other CaCBLs was increased by salt treatment. Several studies have suggested that overlapping signaling pathways operate in response to drought and salinity. Apart from CaCBL5, expression of all other chickpea CBL genes under PEG treatment followed same pattern of their expression in response to salinity. Expressions of CaCBL6, -4 and -8 were either downregulated or unaltered under PEG treatment. In contrast to salt treatment, dehydration enhanced expression of CaCBL5 by ten fold. CaCBL10 registered the highest fold (16-fold) of expression under salinity, while its expression under dehydration was periodically upregulated only at 1hr and 12hr. CaCBL1 registered the highest fold (17-fold) of expression under dehydration at 24hr (Fig 5B).

Bottom Line: Most of the CaCBL genes exhibited high expression in flowers.Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance.These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

ABSTRACT
Calcium ion (Ca2+) is a ubiquitous second messenger that transmits various internal and external signals including stresses and, therefore, is important for plants' response process. Calcineurin B-like proteins (CBLs) are one of the plant calcium sensors, which sense and convey the changes in cytosolic Ca2+-concentration for response process. A search in four leguminous plant (soybean, Medicago truncatula, common bean and chickpea) genomes identified 9 to 15 genes in each species that encode CBL proteins. Sequence analyses of CBL peptides and coding sequences (CDS) suggested that there are nine original CBL genes in these legumes and some of them were multiplied during whole genome or local gene duplication. Coding sequences of chickpea CBL genes (CaCBL) were cloned from their cDNAs and sequenced, and their annotations in the genome assemblies were corrected accordingly. Analyses of protein sequences and gene structures of CBL family in plant kingdom indicated its diverse origin but showed a remarkable conservation in overall protein structure with appearance of complex gene structure in the course of evolution. Expression of CaCBL genes in different tissues and in response to different stress and hormone treatment were studied. Most of the CaCBL genes exhibited high expression in flowers. Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance. These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.

No MeSH data available.