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Percutaneous ultrasound guided implantation of VX2 for creation of a rabbit hepatic tumor model.

White SB, Chen J, Gordon AC, Harris KR, Nicolai JR, West DL, Larson AC - PLoS ONE (2015)

Bottom Line: Open surgical procedures are invasive and require long procedure times and recovery that can result in post-operative morbidity and mortality.Magnetic resonance imaging was used to assess for tumor growth and necropsy was performed to determine rates of tract seeding and metastatic disease.Therefore, percutaneous ultrasound guided tumor implantation safely provides reliable tumor growth for establishing hepatic VX2 tumors in a rabbit model with decreased rates of tract seeding, compared to previously reported methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Division of Interventional Radiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America; Department of Radiology, Northwestern University, Chicago, Illinois, United States of America.

ABSTRACT
Creation of a VX2 tumor model has traditionally required a laparotomy and surgical implantation of tumor fragments. Open surgical procedures are invasive and require long procedure times and recovery that can result in post-operative morbidity and mortality. The purpose of this study is to report the results of a percutaneous ultrasound guided method for creation of a VX2 model in rabbit livers. A total of 27 New Zealand white rabbits underwent a percutaneous ultrasound guided approach, where a VX2 tumor fragment was implanted in the liver. Magnetic resonance imaging was used to assess for tumor growth and necropsy was performed to determine rates of tract seeding and metastatic disease. Ultrasound guided tumor implantation was successful in all 27 rabbits. One rabbit died 2 days following the implantation procedure. Two rabbits had no tumors seen on follow-up imaging. Therefore, tumor development was seen in 24/26 (92%) rabbits. During the follow-up period, tract seeding was seen in 8% of rabbits and 38% had extra-hepatic metastatic disease. Therefore, percutaneous ultrasound guided tumor implantation safely provides reliable tumor growth for establishing hepatic VX2 tumors in a rabbit model with decreased rates of tract seeding, compared to previously reported methods.

No MeSH data available.


Related in: MedlinePlus

Histology.H&E staining was performed confirming tumors within the liver.
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pone.0123888.g004: Histology.H&E staining was performed confirming tumors within the liver.

Mentions: At necropsy, the livers were explanted and assessed for tumor size and location (Fig 3B). The rabbits were also assessed for the presence of metastatic disease on gross pathological examination and size, location and extent of disease were recorded. The liver tumors were then sectioned and sent for hematoxylin and eosin (H&E) staining (Fig 4).


Percutaneous ultrasound guided implantation of VX2 for creation of a rabbit hepatic tumor model.

White SB, Chen J, Gordon AC, Harris KR, Nicolai JR, West DL, Larson AC - PLoS ONE (2015)

Histology.H&E staining was performed confirming tumors within the liver.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390313&req=5

pone.0123888.g004: Histology.H&E staining was performed confirming tumors within the liver.
Mentions: At necropsy, the livers were explanted and assessed for tumor size and location (Fig 3B). The rabbits were also assessed for the presence of metastatic disease on gross pathological examination and size, location and extent of disease were recorded. The liver tumors were then sectioned and sent for hematoxylin and eosin (H&E) staining (Fig 4).

Bottom Line: Open surgical procedures are invasive and require long procedure times and recovery that can result in post-operative morbidity and mortality.Magnetic resonance imaging was used to assess for tumor growth and necropsy was performed to determine rates of tract seeding and metastatic disease.Therefore, percutaneous ultrasound guided tumor implantation safely provides reliable tumor growth for establishing hepatic VX2 tumors in a rabbit model with decreased rates of tract seeding, compared to previously reported methods.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Division of Interventional Radiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America; Department of Radiology, Northwestern University, Chicago, Illinois, United States of America.

ABSTRACT
Creation of a VX2 tumor model has traditionally required a laparotomy and surgical implantation of tumor fragments. Open surgical procedures are invasive and require long procedure times and recovery that can result in post-operative morbidity and mortality. The purpose of this study is to report the results of a percutaneous ultrasound guided method for creation of a VX2 model in rabbit livers. A total of 27 New Zealand white rabbits underwent a percutaneous ultrasound guided approach, where a VX2 tumor fragment was implanted in the liver. Magnetic resonance imaging was used to assess for tumor growth and necropsy was performed to determine rates of tract seeding and metastatic disease. Ultrasound guided tumor implantation was successful in all 27 rabbits. One rabbit died 2 days following the implantation procedure. Two rabbits had no tumors seen on follow-up imaging. Therefore, tumor development was seen in 24/26 (92%) rabbits. During the follow-up period, tract seeding was seen in 8% of rabbits and 38% had extra-hepatic metastatic disease. Therefore, percutaneous ultrasound guided tumor implantation safely provides reliable tumor growth for establishing hepatic VX2 tumors in a rabbit model with decreased rates of tract seeding, compared to previously reported methods.

No MeSH data available.


Related in: MedlinePlus