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Increased biodiversity in the environment improves the humoral response of rats.

Pi C, Allott EH, Ren D, Poulton S, Lee SY, Perkins S, Everett ML, Holzknecht ZE, Lin SS, Parker W - PLoS ONE (2015)

Bottom Line: This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors.However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals.However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, United States of America.

ABSTRACT
Previous studies have compared the immune systems of wild and of laboratory rodents in an effort to determine how laboratory rodents differ from their naturally occurring relatives. This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors. However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals. In this study, the humoral immune responses of laboratory rats in a traditional laboratory environment and in an environment with enriched biodiversity were examined following immunization with a panel of antigens. Biodiversity enrichment included colonization of the laboratory animals with helminths and co-housing the laboratory animals with wild-caught rats. Increased biodiversity did not apparently affect the IgE response to peanut antigens following immunization with those antigens. However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

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Relative concentration of DNP-specific antibody in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats.The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM, (B) IgG, and (C) subclasses of IgG are shown. The means and standard errors are shown. The p-values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)
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pone.0120255.g004: Relative concentration of DNP-specific antibody in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats.The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM, (B) IgG, and (C) subclasses of IgG are shown. The means and standard errors are shown. The p-values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)

Mentions: The level of anti-DNP IgM and IgG in serum following immunization of biome depleted and biome enriched animals was assessed as a measure of the immune response against DNP-Ficoll, a T-independent antigen. The mean concentrations of anti-DNP IgM (Fig. 4A) and anti-DNP IgG (Fig. 4B) were significantly greater in biome enriched animals compared to biome depleted animals (p < 0.0001 and p = 0.039, respectively) following immunization.


Increased biodiversity in the environment improves the humoral response of rats.

Pi C, Allott EH, Ren D, Poulton S, Lee SY, Perkins S, Everett ML, Holzknecht ZE, Lin SS, Parker W - PLoS ONE (2015)

Relative concentration of DNP-specific antibody in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats.The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM, (B) IgG, and (C) subclasses of IgG are shown. The means and standard errors are shown. The p-values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390306&req=5

pone.0120255.g004: Relative concentration of DNP-specific antibody in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats.The relative concentration of antibody was determined by ELISA as described in the Methods. Relative levels of (A) IgM, (B) IgG, and (C) subclasses of IgG are shown. The means and standard errors are shown. The p-values associated with comparing data from biome depleted and biome enriched animals using a t-test are shown. (NS = not significant)
Mentions: The level of anti-DNP IgM and IgG in serum following immunization of biome depleted and biome enriched animals was assessed as a measure of the immune response against DNP-Ficoll, a T-independent antigen. The mean concentrations of anti-DNP IgM (Fig. 4A) and anti-DNP IgG (Fig. 4B) were significantly greater in biome enriched animals compared to biome depleted animals (p < 0.0001 and p = 0.039, respectively) following immunization.

Bottom Line: This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors.However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals.However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, United States of America.

ABSTRACT
Previous studies have compared the immune systems of wild and of laboratory rodents in an effort to determine how laboratory rodents differ from their naturally occurring relatives. This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors. However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals. In this study, the humoral immune responses of laboratory rats in a traditional laboratory environment and in an environment with enriched biodiversity were examined following immunization with a panel of antigens. Biodiversity enrichment included colonization of the laboratory animals with helminths and co-housing the laboratory animals with wild-caught rats. Increased biodiversity did not apparently affect the IgE response to peanut antigens following immunization with those antigens. However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

Show MeSH