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Increased biodiversity in the environment improves the humoral response of rats.

Pi C, Allott EH, Ren D, Poulton S, Lee SY, Perkins S, Everett ML, Holzknecht ZE, Lin SS, Parker W - PLoS ONE (2015)

Bottom Line: This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors.However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals.However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, United States of America.

ABSTRACT
Previous studies have compared the immune systems of wild and of laboratory rodents in an effort to determine how laboratory rodents differ from their naturally occurring relatives. This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors. However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals. In this study, the humoral immune responses of laboratory rats in a traditional laboratory environment and in an environment with enriched biodiversity were examined following immunization with a panel of antigens. Biodiversity enrichment included colonization of the laboratory animals with helminths and co-housing the laboratory animals with wild-caught rats. Increased biodiversity did not apparently affect the IgE response to peanut antigens following immunization with those antigens. However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

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Relative concentration of IgE and of peanut-specific IgE in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats following immunization.The relative concentration of antibody was determined by ELISA as described in the Methods. The means and standard errors are indicated by the horizontal bars, and the data were assessed using a t-test. (A) Following immunization, no statistically significant difference (NS) was observed when comparing total serum IgE levels from biome depleted and biome enriched animals. In addition, (B), following immunization, no statistically significant difference (NS) was observed when comparing anti-peanut IgE levels from biome depleted and biome enriched animals.
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pone.0120255.g002: Relative concentration of IgE and of peanut-specific IgE in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats following immunization.The relative concentration of antibody was determined by ELISA as described in the Methods. The means and standard errors are indicated by the horizontal bars, and the data were assessed using a t-test. (A) Following immunization, no statistically significant difference (NS) was observed when comparing total serum IgE levels from biome depleted and biome enriched animals. In addition, (B), following immunization, no statistically significant difference (NS) was observed when comparing anti-peanut IgE levels from biome depleted and biome enriched animals.

Mentions: As shown in Fig. 2A and in Table 1, no statistically significant difference was observed between serum IgE levels in biome depleted versus biome enriched rats following immunization. Similarly, no statistically significant difference was observed between anti-peanut IgE levels in biome depleted versus biome enriched rats following immunization (Fig. 2B).


Increased biodiversity in the environment improves the humoral response of rats.

Pi C, Allott EH, Ren D, Poulton S, Lee SY, Perkins S, Everett ML, Holzknecht ZE, Lin SS, Parker W - PLoS ONE (2015)

Relative concentration of IgE and of peanut-specific IgE in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats following immunization.The relative concentration of antibody was determined by ELISA as described in the Methods. The means and standard errors are indicated by the horizontal bars, and the data were assessed using a t-test. (A) Following immunization, no statistically significant difference (NS) was observed when comparing total serum IgE levels from biome depleted and biome enriched animals. In addition, (B), following immunization, no statistically significant difference (NS) was observed when comparing anti-peanut IgE levels from biome depleted and biome enriched animals.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390306&req=5

pone.0120255.g002: Relative concentration of IgE and of peanut-specific IgE in the serum of biome depleted (n = 20) and biome enriched (n = 15) rats following immunization.The relative concentration of antibody was determined by ELISA as described in the Methods. The means and standard errors are indicated by the horizontal bars, and the data were assessed using a t-test. (A) Following immunization, no statistically significant difference (NS) was observed when comparing total serum IgE levels from biome depleted and biome enriched animals. In addition, (B), following immunization, no statistically significant difference (NS) was observed when comparing anti-peanut IgE levels from biome depleted and biome enriched animals.
Mentions: As shown in Fig. 2A and in Table 1, no statistically significant difference was observed between serum IgE levels in biome depleted versus biome enriched rats following immunization. Similarly, no statistically significant difference was observed between anti-peanut IgE levels in biome depleted versus biome enriched rats following immunization (Fig. 2B).

Bottom Line: This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors.However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals.However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, United States of America.

ABSTRACT
Previous studies have compared the immune systems of wild and of laboratory rodents in an effort to determine how laboratory rodents differ from their naturally occurring relatives. This comparison serves as an indicator of what sorts of changes might exist between modern humans living in Western culture compared to our hunter-gatherer ancestors. However, immunological experiments on wild-caught animals are difficult and potentially confounded by increased levels of stress in the captive animals. In this study, the humoral immune responses of laboratory rats in a traditional laboratory environment and in an environment with enriched biodiversity were examined following immunization with a panel of antigens. Biodiversity enrichment included colonization of the laboratory animals with helminths and co-housing the laboratory animals with wild-caught rats. Increased biodiversity did not apparently affect the IgE response to peanut antigens following immunization with those antigens. However, animals housed in the enriched biodiversity setting demonstrated an increased mean humoral response to T-independent and T-dependent antigens and increased levels of "natural" antibodies directed at a xenogeneic protein and at an autologous tissue extract that were not used as immunogens.

Show MeSH