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Ciprofloxacin-eluting nanofibers inhibits biofilm formation by Pseudomonas aeruginosa and a methicillin-resistant Staphylococcus aureus.

Ahire JJ, Neveling DP, Hattingh M, Dicks LM - PLoS ONE (2015)

Bottom Line: A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO.No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F.This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, Matieland (Stellenbosch), South Africa.

ABSTRACT
Pseudomonas aeruginosa and Staphylococcus aureus are commonly associated with hospital-acquired infections and are known to form biofilms. Ciprofloxacin (CIP), which is normally used to treat these infections, is seldom effective in killing cells in a biofilm. This is mostly due to slow or weak penetration of CIP to the core of biofilms. The problem is accentuated by the release of CIP below MIC (minimal inhibitory concentration) levels following a rapid (burst) release. The aim of this study was to develop a drug carrier that would keep CIP above MIC levels for an extended period. Ciprofloxacin was suspended into poly(D,L-lactide) (PDLLA) and poly(ethylene oxide) (PEO), and electrospun into nanofibers (CIP-F). All of the CIP was released from the nanofibers within 2 h, which is typical of a burst release. However, 99% of P. aeruginosa PA01 cells and 91% of S. aureus Xen 30 cells (a methicillin-resistant strain) in biofilms were killed when exposed to CIP-F. CIP levels remained above MIC for 5 days, as shown by growth inhibition of the cells in vitro. The nanofibers were smooth in texture with no bead formation, as revealed by scanning electron and atomic force microscopy. A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO. No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F. This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

No MeSH data available.


Related in: MedlinePlus

Survival of MCF-12A breast epithelial cells after treatment with ciprofloxacin (CIP)-containing nanofibers (CIP-F) and nanofibers without CIP (CF).Freshly prepared culture medium served as a negative control (NC) and Triton X-100 as positive control (PC). Data points presented are the average of three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.
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pone.0123648.g005: Survival of MCF-12A breast epithelial cells after treatment with ciprofloxacin (CIP)-containing nanofibers (CIP-F) and nanofibers without CIP (CF).Freshly prepared culture medium served as a negative control (NC) and Triton X-100 as positive control (PC). Data points presented are the average of three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.

Mentions: No significant difference in cell viability was observed when breast epithelial cells MCF-12A were exposed to CF, CIP-F and in the absence of nanofibers (Fig 5). Almost no cells survived exposure to Triton X-100 (Fig 5).


Ciprofloxacin-eluting nanofibers inhibits biofilm formation by Pseudomonas aeruginosa and a methicillin-resistant Staphylococcus aureus.

Ahire JJ, Neveling DP, Hattingh M, Dicks LM - PLoS ONE (2015)

Survival of MCF-12A breast epithelial cells after treatment with ciprofloxacin (CIP)-containing nanofibers (CIP-F) and nanofibers without CIP (CF).Freshly prepared culture medium served as a negative control (NC) and Triton X-100 as positive control (PC). Data points presented are the average of three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390291&req=5

pone.0123648.g005: Survival of MCF-12A breast epithelial cells after treatment with ciprofloxacin (CIP)-containing nanofibers (CIP-F) and nanofibers without CIP (CF).Freshly prepared culture medium served as a negative control (NC) and Triton X-100 as positive control (PC). Data points presented are the average of three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.
Mentions: No significant difference in cell viability was observed when breast epithelial cells MCF-12A were exposed to CF, CIP-F and in the absence of nanofibers (Fig 5). Almost no cells survived exposure to Triton X-100 (Fig 5).

Bottom Line: A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO.No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F.This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, Matieland (Stellenbosch), South Africa.

ABSTRACT
Pseudomonas aeruginosa and Staphylococcus aureus are commonly associated with hospital-acquired infections and are known to form biofilms. Ciprofloxacin (CIP), which is normally used to treat these infections, is seldom effective in killing cells in a biofilm. This is mostly due to slow or weak penetration of CIP to the core of biofilms. The problem is accentuated by the release of CIP below MIC (minimal inhibitory concentration) levels following a rapid (burst) release. The aim of this study was to develop a drug carrier that would keep CIP above MIC levels for an extended period. Ciprofloxacin was suspended into poly(D,L-lactide) (PDLLA) and poly(ethylene oxide) (PEO), and electrospun into nanofibers (CIP-F). All of the CIP was released from the nanofibers within 2 h, which is typical of a burst release. However, 99% of P. aeruginosa PA01 cells and 91% of S. aureus Xen 30 cells (a methicillin-resistant strain) in biofilms were killed when exposed to CIP-F. CIP levels remained above MIC for 5 days, as shown by growth inhibition of the cells in vitro. The nanofibers were smooth in texture with no bead formation, as revealed by scanning electron and atomic force microscopy. A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO. No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F. This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

No MeSH data available.


Related in: MedlinePlus