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Ciprofloxacin-eluting nanofibers inhibits biofilm formation by Pseudomonas aeruginosa and a methicillin-resistant Staphylococcus aureus.

Ahire JJ, Neveling DP, Hattingh M, Dicks LM - PLoS ONE (2015)

Bottom Line: A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO.No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F.This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, Matieland (Stellenbosch), South Africa.

ABSTRACT
Pseudomonas aeruginosa and Staphylococcus aureus are commonly associated with hospital-acquired infections and are known to form biofilms. Ciprofloxacin (CIP), which is normally used to treat these infections, is seldom effective in killing cells in a biofilm. This is mostly due to slow or weak penetration of CIP to the core of biofilms. The problem is accentuated by the release of CIP below MIC (minimal inhibitory concentration) levels following a rapid (burst) release. The aim of this study was to develop a drug carrier that would keep CIP above MIC levels for an extended period. Ciprofloxacin was suspended into poly(D,L-lactide) (PDLLA) and poly(ethylene oxide) (PEO), and electrospun into nanofibers (CIP-F). All of the CIP was released from the nanofibers within 2 h, which is typical of a burst release. However, 99% of P. aeruginosa PA01 cells and 91% of S. aureus Xen 30 cells (a methicillin-resistant strain) in biofilms were killed when exposed to CIP-F. CIP levels remained above MIC for 5 days, as shown by growth inhibition of the cells in vitro. The nanofibers were smooth in texture with no bead formation, as revealed by scanning electron and atomic force microscopy. A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO. No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F. This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

No MeSH data available.


Related in: MedlinePlus

A: Release of ciprofloxacin (CIP) from PDLLA: PEO nanofibers immediately after immersion into PBS, and 2, 3, 4, 6, 24 and 48 h thereafter, B: in vitro antimicrobial activity of CIP-containing nanofibers (CIP-F) against P. aeruginosa PA01 and S. aureus Xen 30, C: cell density of P. aeruginosa PA01 exposed to nanofibers without CIP (CF) and D: cell density of S. aureus Xen 30 exposed to CF and CIP-F.The control was without nanofibers and without CIP. Data points represent an average reading recorded from three disks per time point and three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.
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pone.0123648.g003: A: Release of ciprofloxacin (CIP) from PDLLA: PEO nanofibers immediately after immersion into PBS, and 2, 3, 4, 6, 24 and 48 h thereafter, B: in vitro antimicrobial activity of CIP-containing nanofibers (CIP-F) against P. aeruginosa PA01 and S. aureus Xen 30, C: cell density of P. aeruginosa PA01 exposed to nanofibers without CIP (CF) and D: cell density of S. aureus Xen 30 exposed to CF and CIP-F.The control was without nanofibers and without CIP. Data points represent an average reading recorded from three disks per time point and three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.

Mentions: Immediately after electrospinning, 6.01 μg CIP was released from 2 mg CIP-F (Fig 3A). Two hours later, 58.22 μg CIP was released (Fig 3A). The release of CIP decreased to 2.02 μg after a further 3 h (Fig 3A). All of the CIP was released within the first 3 h (Fig 3A). Despite the rapid diffusion of CIP from CIP-F, growth of P. aeruginosa PA01 was inhibited for seven consecutive days, as observed after seven transfers of CIP-F disks onto seeded plates (Fig 3B). Growth of P. aeruginosa PA01 was severely inhibited within the first 24 h, as shown by an inhibition zone of 35 mm in diameter (Fig 3B).


Ciprofloxacin-eluting nanofibers inhibits biofilm formation by Pseudomonas aeruginosa and a methicillin-resistant Staphylococcus aureus.

Ahire JJ, Neveling DP, Hattingh M, Dicks LM - PLoS ONE (2015)

A: Release of ciprofloxacin (CIP) from PDLLA: PEO nanofibers immediately after immersion into PBS, and 2, 3, 4, 6, 24 and 48 h thereafter, B: in vitro antimicrobial activity of CIP-containing nanofibers (CIP-F) against P. aeruginosa PA01 and S. aureus Xen 30, C: cell density of P. aeruginosa PA01 exposed to nanofibers without CIP (CF) and D: cell density of S. aureus Xen 30 exposed to CF and CIP-F.The control was without nanofibers and without CIP. Data points represent an average reading recorded from three disks per time point and three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390291&req=5

pone.0123648.g003: A: Release of ciprofloxacin (CIP) from PDLLA: PEO nanofibers immediately after immersion into PBS, and 2, 3, 4, 6, 24 and 48 h thereafter, B: in vitro antimicrobial activity of CIP-containing nanofibers (CIP-F) against P. aeruginosa PA01 and S. aureus Xen 30, C: cell density of P. aeruginosa PA01 exposed to nanofibers without CIP (CF) and D: cell density of S. aureus Xen 30 exposed to CF and CIP-F.The control was without nanofibers and without CIP. Data points represent an average reading recorded from three disks per time point and three independent experiments (mean ± standard deviation). * p < 0.05. ns: not significant.
Mentions: Immediately after electrospinning, 6.01 μg CIP was released from 2 mg CIP-F (Fig 3A). Two hours later, 58.22 μg CIP was released (Fig 3A). The release of CIP decreased to 2.02 μg after a further 3 h (Fig 3A). All of the CIP was released within the first 3 h (Fig 3A). Despite the rapid diffusion of CIP from CIP-F, growth of P. aeruginosa PA01 was inhibited for seven consecutive days, as observed after seven transfers of CIP-F disks onto seeded plates (Fig 3B). Growth of P. aeruginosa PA01 was severely inhibited within the first 24 h, as shown by an inhibition zone of 35 mm in diameter (Fig 3B).

Bottom Line: A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO.No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F.This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, University of Stellenbosch, Matieland (Stellenbosch), South Africa.

ABSTRACT
Pseudomonas aeruginosa and Staphylococcus aureus are commonly associated with hospital-acquired infections and are known to form biofilms. Ciprofloxacin (CIP), which is normally used to treat these infections, is seldom effective in killing cells in a biofilm. This is mostly due to slow or weak penetration of CIP to the core of biofilms. The problem is accentuated by the release of CIP below MIC (minimal inhibitory concentration) levels following a rapid (burst) release. The aim of this study was to develop a drug carrier that would keep CIP above MIC levels for an extended period. Ciprofloxacin was suspended into poly(D,L-lactide) (PDLLA) and poly(ethylene oxide) (PEO), and electrospun into nanofibers (CIP-F). All of the CIP was released from the nanofibers within 2 h, which is typical of a burst release. However, 99% of P. aeruginosa PA01 cells and 91% of S. aureus Xen 30 cells (a methicillin-resistant strain) in biofilms were killed when exposed to CIP-F. CIP levels remained above MIC for 5 days, as shown by growth inhibition of the cells in vitro. The nanofibers were smooth in texture with no bead formation, as revealed by scanning electron and atomic force microscopy. A single vibration peak at 1632 cm-1, recorded with Fourier transform infrared spectroscopy, indicated that CIP remained in crystal form when incorporated into PDLLA: PEO. No abnormalities in the histology of MCF-12A breast epithelial cells were observed when exposed to CIP-F. This is the first report of the inhibition of biofilm formation by CIP released from PDLLA: PEO nanofibers.

No MeSH data available.


Related in: MedlinePlus