Total proteome analysis identifies migration defects as a major pathogenetic factor in immunoglobulin heavy chain variable region (IGHV)-unmutated chronic lymphocytic leukemia.
Bottom Line: Furthermore, UM-CLL cells underexpressed proteins associated with cytoskeletal remodeling and overexpressed proteins associated with transcriptional and translational activity.Taken together, our findings indicate that UM-CLL cells are less migratory and more adhesive than M-CLL cells, resulting in their retention in lymph nodes, where they are exposed to proliferative stimuli.Our study illustrates the potential of total proteome analysis to elucidate pathogenetic mechanisms in cancer.
Affiliation: From the ‡Department of Molecular and Clinical Cancer Medicine.Show MeSH
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Mentions: A total of 3521 proteins were identified within a 1% global false discovery rate and with a high confidence of correct peptide sequence assignment (Fig. 1A and the full list of proteins provided in the supplemental data). Of these, 2024 proteins were identified in all three separate iTRAQ-MS experiments (Fig. 1A). To determine whether protein expression was similar between UM-CLL and M-CLL samples, we subjected the data to PCA. As shown in Fig. 1B, PCA showed a distinction between UM-CLL and M-CLL samples based on protein expression.
Affiliation: From the ‡Department of Molecular and Clinical Cancer Medicine.