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Proteomics analyses for the global proteins in the brain tissues of different human prion diseases.

Shi Q, Chen LN, Zhang BY, Xiao K, Zhou W, Chen C, Zhang XM, Tian C, Gao C, Wang J, Han J, Dong XP - Mol. Cell Proteomics (2015)

Bottom Line: Global protein profiling, significant pathway, and functional categories were analyzed.Almost coincident biological functions were identified in the brain tissues of the three diseases.This is the first study to provide a reference proteome map for human prion diseases and will be helpful for future studies focused on potential biomarkers for the diagnosis and therapy of human prion diseases.

View Article: PubMed Central - PubMed

Affiliation: From the ‡State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University, Hangzhou 310003), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Chang-Bai Rd 155, Beijing 102206, People's Republic of China;

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Numbers of the differentially expressed proteins identified in the cortex and cerebellum tissues from sCJD, FFI, and G114V gCJD cases.A, Individual numbers of the up- and down-regulated proteins in the cortex and cerebellum tissues from the groups of sCJD, FFI, and G114V gCJD based on the standard of 1.2-, 1.5-, and 2.0-fold change, respectively. The numbers of the total changed proteins each group are shown on the top of each column and the p value between the groups of cortex and cerebellum (with 1.2-fold changed) is indicated on the top of graph. The exact numbers of the up- and down-regulated proteins each group are shown on the bottom. B, Numbers of the commonly up-regulated and down-regulated proteins (with 1.2-fold changed) in cortex and cerebellum regions of three prion diseases. The p value between the groups of cortex and cerebellum is indicated on the top.
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Figure 1: Numbers of the differentially expressed proteins identified in the cortex and cerebellum tissues from sCJD, FFI, and G114V gCJD cases.A, Individual numbers of the up- and down-regulated proteins in the cortex and cerebellum tissues from the groups of sCJD, FFI, and G114V gCJD based on the standard of 1.2-, 1.5-, and 2.0-fold change, respectively. The numbers of the total changed proteins each group are shown on the top of each column and the p value between the groups of cortex and cerebellum (with 1.2-fold changed) is indicated on the top of graph. The exact numbers of the up- and down-regulated proteins each group are shown on the bottom. B, Numbers of the commonly up-regulated and down-regulated proteins (with 1.2-fold changed) in cortex and cerebellum regions of three prion diseases. The p value between the groups of cortex and cerebellum is indicated on the top.

Mentions: Using the standards of 1.2-, 1.5-, and 2.0-fold change, the potential differentially expressing proteins in each disease were separately counted. The exact numbers of the up- and down-regulated proteins in cortex and cerebellum tissues from the groups of sCJD, FFI, and G114V gCJD were shown in Fig. 1A. The numbers of 1.2-, 1.5-, and 2.0-fold changed proteins in cortical regions were 1178, 403, and 117 in sCJD, 878, 235, and 74 in FFI, 1314, 537 and 183 in G114V gCJD, whereas those in cerebella regions were 1412, 671, and 158 in sCJD, 1356, 622, and 221 in FFI, 1503, 765, and 316 in G114V gCJD, respectively. For exploring the potential changes in proteomic profiles among the different human prion diseases maximally, we used the proteins that were 1.2-fold increased or decreased as the differential expressing proteins in further analyses.


Proteomics analyses for the global proteins in the brain tissues of different human prion diseases.

Shi Q, Chen LN, Zhang BY, Xiao K, Zhou W, Chen C, Zhang XM, Tian C, Gao C, Wang J, Han J, Dong XP - Mol. Cell Proteomics (2015)

Numbers of the differentially expressed proteins identified in the cortex and cerebellum tissues from sCJD, FFI, and G114V gCJD cases.A, Individual numbers of the up- and down-regulated proteins in the cortex and cerebellum tissues from the groups of sCJD, FFI, and G114V gCJD based on the standard of 1.2-, 1.5-, and 2.0-fold change, respectively. The numbers of the total changed proteins each group are shown on the top of each column and the p value between the groups of cortex and cerebellum (with 1.2-fold changed) is indicated on the top of graph. The exact numbers of the up- and down-regulated proteins each group are shown on the bottom. B, Numbers of the commonly up-regulated and down-regulated proteins (with 1.2-fold changed) in cortex and cerebellum regions of three prion diseases. The p value between the groups of cortex and cerebellum is indicated on the top.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 1: Numbers of the differentially expressed proteins identified in the cortex and cerebellum tissues from sCJD, FFI, and G114V gCJD cases.A, Individual numbers of the up- and down-regulated proteins in the cortex and cerebellum tissues from the groups of sCJD, FFI, and G114V gCJD based on the standard of 1.2-, 1.5-, and 2.0-fold change, respectively. The numbers of the total changed proteins each group are shown on the top of each column and the p value between the groups of cortex and cerebellum (with 1.2-fold changed) is indicated on the top of graph. The exact numbers of the up- and down-regulated proteins each group are shown on the bottom. B, Numbers of the commonly up-regulated and down-regulated proteins (with 1.2-fold changed) in cortex and cerebellum regions of three prion diseases. The p value between the groups of cortex and cerebellum is indicated on the top.
Mentions: Using the standards of 1.2-, 1.5-, and 2.0-fold change, the potential differentially expressing proteins in each disease were separately counted. The exact numbers of the up- and down-regulated proteins in cortex and cerebellum tissues from the groups of sCJD, FFI, and G114V gCJD were shown in Fig. 1A. The numbers of 1.2-, 1.5-, and 2.0-fold changed proteins in cortical regions were 1178, 403, and 117 in sCJD, 878, 235, and 74 in FFI, 1314, 537 and 183 in G114V gCJD, whereas those in cerebella regions were 1412, 671, and 158 in sCJD, 1356, 622, and 221 in FFI, 1503, 765, and 316 in G114V gCJD, respectively. For exploring the potential changes in proteomic profiles among the different human prion diseases maximally, we used the proteins that were 1.2-fold increased or decreased as the differential expressing proteins in further analyses.

Bottom Line: Global protein profiling, significant pathway, and functional categories were analyzed.Almost coincident biological functions were identified in the brain tissues of the three diseases.This is the first study to provide a reference proteome map for human prion diseases and will be helpful for future studies focused on potential biomarkers for the diagnosis and therapy of human prion diseases.

View Article: PubMed Central - PubMed

Affiliation: From the ‡State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University, Hangzhou 310003), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Chang-Bai Rd 155, Beijing 102206, People's Republic of China;

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Related in: MedlinePlus