Limits...
MitoFates: improved prediction of mitochondrial targeting sequences and their cleavage sites.

Fukasawa Y, Tsuji J, Fu SC, Tomii K, Horton P, Imai K - Mol. Cell Proteomics (2015)

Bottom Line: Here we describe MitoFates, an improved prediction method for cleavable N-terminal mitochondrial targeting signals (presequences) and their cleavage sites.Interestingly, these include candidate regulators of parkin translocation to damaged mitochondria, and also many genes with known disease mutations, suggesting that careful investigation of MitoFates predictions may be helpful in elucidating the role of mitochondria in health and disease.MitoFates is open source with a convenient web server publicly available.

View Article: PubMed Central - PubMed

Affiliation: From the ‡Department of Computational Biology, Graduate School of Frontier Sciences, The University Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba, 277-8561, Japan;

Show MeSH
An example of prediction output from the MitoFates web server is shown.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4390256&req=5

Figure 5: An example of prediction output from the MitoFates web server is shown.

Mentions: We developed a MitoFates webserver for easy use, available at http://mitf.cbrc.jp/MitoFates/. The default threshold is set to 0.385, corresponding to an estimated 79% precision and 80% recall. The MitoFates webserver can accept multiple protein sequences at a time. The output shows prediction results with predicted cleavage sites (of MPP and secondary proteases) and presequence hexamer motif hits having a maximum PA score (Fig. 5). More information is available at http://mitf.cbrc.jp/MitoFates/usage.html. The source code for MitoFates is also available at the website.


MitoFates: improved prediction of mitochondrial targeting sequences and their cleavage sites.

Fukasawa Y, Tsuji J, Fu SC, Tomii K, Horton P, Imai K - Mol. Cell Proteomics (2015)

An example of prediction output from the MitoFates web server is shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390256&req=5

Figure 5: An example of prediction output from the MitoFates web server is shown.
Mentions: We developed a MitoFates webserver for easy use, available at http://mitf.cbrc.jp/MitoFates/. The default threshold is set to 0.385, corresponding to an estimated 79% precision and 80% recall. The MitoFates webserver can accept multiple protein sequences at a time. The output shows prediction results with predicted cleavage sites (of MPP and secondary proteases) and presequence hexamer motif hits having a maximum PA score (Fig. 5). More information is available at http://mitf.cbrc.jp/MitoFates/usage.html. The source code for MitoFates is also available at the website.

Bottom Line: Here we describe MitoFates, an improved prediction method for cleavable N-terminal mitochondrial targeting signals (presequences) and their cleavage sites.Interestingly, these include candidate regulators of parkin translocation to damaged mitochondria, and also many genes with known disease mutations, suggesting that careful investigation of MitoFates predictions may be helpful in elucidating the role of mitochondria in health and disease.MitoFates is open source with a convenient web server publicly available.

View Article: PubMed Central - PubMed

Affiliation: From the ‡Department of Computational Biology, Graduate School of Frontier Sciences, The University Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba, 277-8561, Japan;

Show MeSH