MitoFates: improved prediction of mitochondrial targeting sequences and their cleavage sites.
Bottom Line: Five-hundred and eighty of these genes were not annotated as mitochondrial in either UniProt or Gene Ontology.Interestingly, these include candidate regulators of parkin translocation to damaged mitochondria, and also many genes with known disease mutations, suggesting that careful investigation of MitoFates predictions may be helpful in elucidating the role of mitochondria in health and disease.MitoFates is open source with a convenient web server publicly available.
Affiliation: From the ‡Department of Computational Biology, Graduate School of Frontier Sciences, The University Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba, 277-8561, Japan;Show MeSH
Mentions: We developed a MitoFates webserver for easy use, available at http://mitf.cbrc.jp/MitoFates/. The default threshold is set to 0.385, corresponding to an estimated 79% precision and 80% recall. The MitoFates webserver can accept multiple protein sequences at a time. The output shows prediction results with predicted cleavage sites (of MPP and secondary proteases) and presequence hexamer motif hits having a maximum PA score (Fig. 5). More information is available at http://mitf.cbrc.jp/MitoFates/usage.html. The source code for MitoFates is also available at the website.
Affiliation: From the ‡Department of Computational Biology, Graduate School of Frontier Sciences, The University Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba, 277-8561, Japan;