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Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions.

Trtikova M, Wikmark OG, Zemp N, Widmer A, Hilbeck A - PLoS ONE (2015)

Bottom Line: Under stressful environmental conditions we found similar transgene expressions as under optimal conditions but Bt content responded differently.These results suggest that Bt content is not only controlled by the transgene expression but is also dependent on the genetic background of the maize variety.Under stressful conditions the concentration of Bt protein is even more difficult to predict.

View Article: PubMed Central - PubMed

Affiliation: ETH Zurich, IBZ, Plant Ecological Genetics, Universitaetstrasse 16, 8092 Zurich, Switzerland.

ABSTRACT
Bt protein content in transgenic insect resistant (Bt) maize may vary between tissues within plants and between plants growing under different environmental conditions. However, it is unknown whether and how Bt protein content correlates with transgene expression, and whether this relationship is influenced by stressful environmental conditions. Two Bt maize varieties containing the same transgene cassette (MON 810) were grown under optimal and stressful conditions. Before and during stress exposure, the upper leaves were analysed for transgene expression using quantitative RT-PCR and for Bt content using ELISA. Under optimal conditions there was no significant difference in the transgene expression between the two investigated Bt maize varieties whereas Bt protein content differed significantly. Transgene expression was correlated with Bt protein content in only one of the varieties. Under stressful environmental conditions we found similar transgene expressions as under optimal conditions but Bt content responded differently. These results suggest that Bt content is not only controlled by the transgene expression but is also dependent on the genetic background of the maize variety. Under stressful conditions the concentration of Bt protein is even more difficult to predict.

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Related in: MedlinePlus

Correlation under optimal conditions.Spearman rank correlation between relative transgene expression and Bt protein content in the plants grown under optimal conditions: A) white Bt maize and B) yellow Bt maize.
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pone.0123011.g003: Correlation under optimal conditions.Spearman rank correlation between relative transgene expression and Bt protein content in the plants grown under optimal conditions: A) white Bt maize and B) yellow Bt maize.

Mentions: The relationship between transgene expression and Bt protein content differed between the two Bt maize varieties. The Bt protein content was correlated with cry1Ab transgene expression in the white Bt maize plants (Rs = 0.536, P = 0.040) but not in the yellow Bt maize plants (Rs = 0.407, P = 0.133) (Fig 3). Furthermore, the correlation was only found in the white Bt maize grown under optimal conditions before any stress treatment was applied. No correlation between cry1Ab transgene expression and Cry1Ab protein content was found in the plants exposed to cold/wet or hot/dry stress (S1 Fig).


Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions.

Trtikova M, Wikmark OG, Zemp N, Widmer A, Hilbeck A - PLoS ONE (2015)

Correlation under optimal conditions.Spearman rank correlation between relative transgene expression and Bt protein content in the plants grown under optimal conditions: A) white Bt maize and B) yellow Bt maize.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390241&req=5

pone.0123011.g003: Correlation under optimal conditions.Spearman rank correlation between relative transgene expression and Bt protein content in the plants grown under optimal conditions: A) white Bt maize and B) yellow Bt maize.
Mentions: The relationship between transgene expression and Bt protein content differed between the two Bt maize varieties. The Bt protein content was correlated with cry1Ab transgene expression in the white Bt maize plants (Rs = 0.536, P = 0.040) but not in the yellow Bt maize plants (Rs = 0.407, P = 0.133) (Fig 3). Furthermore, the correlation was only found in the white Bt maize grown under optimal conditions before any stress treatment was applied. No correlation between cry1Ab transgene expression and Cry1Ab protein content was found in the plants exposed to cold/wet or hot/dry stress (S1 Fig).

Bottom Line: Under stressful environmental conditions we found similar transgene expressions as under optimal conditions but Bt content responded differently.These results suggest that Bt content is not only controlled by the transgene expression but is also dependent on the genetic background of the maize variety.Under stressful conditions the concentration of Bt protein is even more difficult to predict.

View Article: PubMed Central - PubMed

Affiliation: ETH Zurich, IBZ, Plant Ecological Genetics, Universitaetstrasse 16, 8092 Zurich, Switzerland.

ABSTRACT
Bt protein content in transgenic insect resistant (Bt) maize may vary between tissues within plants and between plants growing under different environmental conditions. However, it is unknown whether and how Bt protein content correlates with transgene expression, and whether this relationship is influenced by stressful environmental conditions. Two Bt maize varieties containing the same transgene cassette (MON 810) were grown under optimal and stressful conditions. Before and during stress exposure, the upper leaves were analysed for transgene expression using quantitative RT-PCR and for Bt content using ELISA. Under optimal conditions there was no significant difference in the transgene expression between the two investigated Bt maize varieties whereas Bt protein content differed significantly. Transgene expression was correlated with Bt protein content in only one of the varieties. Under stressful environmental conditions we found similar transgene expressions as under optimal conditions but Bt content responded differently. These results suggest that Bt content is not only controlled by the transgene expression but is also dependent on the genetic background of the maize variety. Under stressful conditions the concentration of Bt protein is even more difficult to predict.

No MeSH data available.


Related in: MedlinePlus