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Phylogenetic molecular species delimitations unravel potential new species in the pest genus Spodoptera Guenée, 1852 (Lepidoptera, Noctuidae).

Dumas P, Barbut J, Le Ru B, Silvain JF, Clamens AL, d'Alençon E, Kergoat GJ - PLoS ONE (2015)

Bottom Line: As some of them can efficiently deal with large single-locus datasets, they could speed up the process of species discovery compared to more time consuming molecular methods, and benefit from the existence of large public datasets; these methods can also particularly favour scientific research and actions dealing with threatened or economically important taxa.In this work, the effectiveness of innovative (and faster) species delimitation approaches to infer putative species boundaries has been successfully tested in Spodoptera, by processing the most comprehensive dataset (in terms of number of species and specimens) ever achieved; results are congruent and reliable, irrespective of the set of parameters and phylogenetic models applied.This case study highlights the interest of molecular species delimitation methods as valuable tools for species discovery and to emphasize taxonomic ambiguities.

View Article: PubMed Central - PubMed

Affiliation: Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands.

ABSTRACT
Nowadays molecular species delimitation methods promote the identification of species boundaries within complex taxonomic groups by adopting innovative species concepts and theories (e.g. branching patterns, coalescence). As some of them can efficiently deal with large single-locus datasets, they could speed up the process of species discovery compared to more time consuming molecular methods, and benefit from the existence of large public datasets; these methods can also particularly favour scientific research and actions dealing with threatened or economically important taxa. In this study we aim to investigate and clarify the status of economically important moths species belonging to the genus Spodoptera (Lepidoptera, Noctuidae), a complex group in which previous phylogenetic analyses and integrative approaches already suggested the possible occurrence of cryptic species and taxonomic ambiguities. In this work, the effectiveness of innovative (and faster) species delimitation approaches to infer putative species boundaries has been successfully tested in Spodoptera, by processing the most comprehensive dataset (in terms of number of species and specimens) ever achieved; results are congruent and reliable, irrespective of the set of parameters and phylogenetic models applied. Our analyses confirm the existence of three potential new species clusters (for S. exigua (Hübner, 1808), S. frugiperda (J.E. Smith, 1797) and S. mauritia (Boisduval, 1833)) and support the synonymy of S. marima (Schaus, 1904) with S. ornithogalli (Guenée, 1852). They also highlight the ambiguity of the status of S. cosmiodes (Walker, 1858) and S. descoinsi Lalanne-Cassou & Silvain, 1994. This case study highlights the interest of molecular species delimitation methods as valuable tools for species discovery and to emphasize taxonomic ambiguities.

No MeSH data available.


COI-based phylogenetic relationships of Spodoptera species.The tree on the left summarizes the results of ML analyses while the tree on the right summarizes the results of BI analyses. Black dots indicate nodes supported by bootstrap values ≥ 70% (ML tree) or supported by posterior probability ≥ 0.95 (BI tree).
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pone.0122407.g001: COI-based phylogenetic relationships of Spodoptera species.The tree on the left summarizes the results of ML analyses while the tree on the right summarizes the results of BI analyses. Black dots indicate nodes supported by bootstrap values ≥ 70% (ML tree) or supported by posterior probability ≥ 0.95 (BI tree).

Mentions: The phylogenetic analyses in ML and BI show similar results (Fig 1; see also S1 and S2 Figs for details), which only differ by the position of some outgroups and the placement of two Spodoptera species (S. apertura and S. pecten). In both ML and BI analyses, the genus Spodoptera is recovered monophyletic. Support for Spodoptera monophyly was higher for the ML tree (BV of 95%) in comparison to the BI tree (PP of 0.94). The phylogenies are overall not resolved, except for the terminal nodes. Support values are equally high for the nodes leading to morphospecies (BV ≥ 70% for 23 out of the 27 corresponding nodes under ML; PP ≥ 0.95 for 21 out of the 27 corresponding nodes under BI).


Phylogenetic molecular species delimitations unravel potential new species in the pest genus Spodoptera Guenée, 1852 (Lepidoptera, Noctuidae).

Dumas P, Barbut J, Le Ru B, Silvain JF, Clamens AL, d'Alençon E, Kergoat GJ - PLoS ONE (2015)

COI-based phylogenetic relationships of Spodoptera species.The tree on the left summarizes the results of ML analyses while the tree on the right summarizes the results of BI analyses. Black dots indicate nodes supported by bootstrap values ≥ 70% (ML tree) or supported by posterior probability ≥ 0.95 (BI tree).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390195&req=5

pone.0122407.g001: COI-based phylogenetic relationships of Spodoptera species.The tree on the left summarizes the results of ML analyses while the tree on the right summarizes the results of BI analyses. Black dots indicate nodes supported by bootstrap values ≥ 70% (ML tree) or supported by posterior probability ≥ 0.95 (BI tree).
Mentions: The phylogenetic analyses in ML and BI show similar results (Fig 1; see also S1 and S2 Figs for details), which only differ by the position of some outgroups and the placement of two Spodoptera species (S. apertura and S. pecten). In both ML and BI analyses, the genus Spodoptera is recovered monophyletic. Support for Spodoptera monophyly was higher for the ML tree (BV of 95%) in comparison to the BI tree (PP of 0.94). The phylogenies are overall not resolved, except for the terminal nodes. Support values are equally high for the nodes leading to morphospecies (BV ≥ 70% for 23 out of the 27 corresponding nodes under ML; PP ≥ 0.95 for 21 out of the 27 corresponding nodes under BI).

Bottom Line: As some of them can efficiently deal with large single-locus datasets, they could speed up the process of species discovery compared to more time consuming molecular methods, and benefit from the existence of large public datasets; these methods can also particularly favour scientific research and actions dealing with threatened or economically important taxa.In this work, the effectiveness of innovative (and faster) species delimitation approaches to infer putative species boundaries has been successfully tested in Spodoptera, by processing the most comprehensive dataset (in terms of number of species and specimens) ever achieved; results are congruent and reliable, irrespective of the set of parameters and phylogenetic models applied.This case study highlights the interest of molecular species delimitation methods as valuable tools for species discovery and to emphasize taxonomic ambiguities.

View Article: PubMed Central - PubMed

Affiliation: Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands.

ABSTRACT
Nowadays molecular species delimitation methods promote the identification of species boundaries within complex taxonomic groups by adopting innovative species concepts and theories (e.g. branching patterns, coalescence). As some of them can efficiently deal with large single-locus datasets, they could speed up the process of species discovery compared to more time consuming molecular methods, and benefit from the existence of large public datasets; these methods can also particularly favour scientific research and actions dealing with threatened or economically important taxa. In this study we aim to investigate and clarify the status of economically important moths species belonging to the genus Spodoptera (Lepidoptera, Noctuidae), a complex group in which previous phylogenetic analyses and integrative approaches already suggested the possible occurrence of cryptic species and taxonomic ambiguities. In this work, the effectiveness of innovative (and faster) species delimitation approaches to infer putative species boundaries has been successfully tested in Spodoptera, by processing the most comprehensive dataset (in terms of number of species and specimens) ever achieved; results are congruent and reliable, irrespective of the set of parameters and phylogenetic models applied. Our analyses confirm the existence of three potential new species clusters (for S. exigua (Hübner, 1808), S. frugiperda (J.E. Smith, 1797) and S. mauritia (Boisduval, 1833)) and support the synonymy of S. marima (Schaus, 1904) with S. ornithogalli (Guenée, 1852). They also highlight the ambiguity of the status of S. cosmiodes (Walker, 1858) and S. descoinsi Lalanne-Cassou & Silvain, 1994. This case study highlights the interest of molecular species delimitation methods as valuable tools for species discovery and to emphasize taxonomic ambiguities.

No MeSH data available.