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Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

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Related in: MedlinePlus

Immunoreactivity against receptors in the brainstem of taiep and Sprague-Dawley rats 6 months old. Paraffin-included slices of 3 μm were immunostained with rabbit monoclonal anti-CCR2, anti-CCR5, anti-CXCR2, and anti-CXCR4 (fluorescein, green). Propidium iodide (red) or DAPI (blue) was used as nuclear counterstaining. SD; Sprague-Dawley rats.
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fig5: Immunoreactivity against receptors in the brainstem of taiep and Sprague-Dawley rats 6 months old. Paraffin-included slices of 3 μm were immunostained with rabbit monoclonal anti-CCR2, anti-CCR5, anti-CXCR2, and anti-CXCR4 (fluorescein, green). Propidium iodide (red) or DAPI (blue) was used as nuclear counterstaining. SD; Sprague-Dawley rats.

Mentions: Immunofluorescence studies showed decreased intensity against CCR5 and CXCR4 in the brainstem of 6-month-old taiep rats in comparison with SD rats. CCR5 immunostaining was evident in glial cells located in the white matter of both SD and taiep rats. CCR2 and CXCR2 were found in glial cells and neurons, but CXCR4 was observed in glial cells (Figure 5). CXCR2 immunofluorescence intensity in taiep rats was not different from that in SD rats (Figure 5).


Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Immunoreactivity against receptors in the brainstem of taiep and Sprague-Dawley rats 6 months old. Paraffin-included slices of 3 μm were immunostained with rabbit monoclonal anti-CCR2, anti-CCR5, anti-CXCR2, and anti-CXCR4 (fluorescein, green). Propidium iodide (red) or DAPI (blue) was used as nuclear counterstaining. SD; Sprague-Dawley rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390177&req=5

fig5: Immunoreactivity against receptors in the brainstem of taiep and Sprague-Dawley rats 6 months old. Paraffin-included slices of 3 μm were immunostained with rabbit monoclonal anti-CCR2, anti-CCR5, anti-CXCR2, and anti-CXCR4 (fluorescein, green). Propidium iodide (red) or DAPI (blue) was used as nuclear counterstaining. SD; Sprague-Dawley rats.
Mentions: Immunofluorescence studies showed decreased intensity against CCR5 and CXCR4 in the brainstem of 6-month-old taiep rats in comparison with SD rats. CCR5 immunostaining was evident in glial cells located in the white matter of both SD and taiep rats. CCR2 and CXCR2 were found in glial cells and neurons, but CXCR4 was observed in glial cells (Figure 5). CXCR2 immunofluorescence intensity in taiep rats was not different from that in SD rats (Figure 5).

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

Show MeSH
Related in: MedlinePlus