Limits...
Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

Show MeSH

Related in: MedlinePlus

Protein levels of chemokines and their receptors in the brainstem of Sprague-Dawley (SD) and taiep rats. Chemokines, receptors, and growth factors were assayed using indirect ELISA. Each value represents the mean ± SEM of 5 independent experiments made in triplicate. ∗Significantly different from SD rats (Student's t-test). The significance was established at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4390177&req=5

fig4: Protein levels of chemokines and their receptors in the brainstem of Sprague-Dawley (SD) and taiep rats. Chemokines, receptors, and growth factors were assayed using indirect ELISA. Each value represents the mean ± SEM of 5 independent experiments made in triplicate. ∗Significantly different from SD rats (Student's t-test). The significance was established at P < 0.05.

Mentions: Comparison of protein levels between taiep rats and SD rats showed changes in chemokine receptors and growth factors at both ages. At 1 month of age, receptors that increased were CCR2 (43.5 ± 5.1%), CCR8 (111.3 ± 12.4%), CXCR4 (115.8 ± 18.1%), and FGF2 (27.6% ± 4.1). In contrast, CCR5 (−19.8 ± 5.1) and CXCL1 (−82.2 ± 1.6%) were decreased (Figure 4). In the brainstem of 6-month-old rats, protein levels were decreased for CCL2 (−39.0% ± 5.5%), CXCL1 (−80.9% ± 3.6%), CCR1 (−14.4% ± 7.9%), CCR2 (−41.2% ± 1.4%), CCR5 (−21.8% ± 5.8%), CCR8 (−34.6% ± 5.8%), CXCR4 (−54.4% ± 3.7%), and, the growth factor, FGF2 (−42.7% ± 2.7%). In contrast, VEGFA (125.5% ± 29.7%) levels increased (Figure 4).


Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Protein levels of chemokines and their receptors in the brainstem of Sprague-Dawley (SD) and taiep rats. Chemokines, receptors, and growth factors were assayed using indirect ELISA. Each value represents the mean ± SEM of 5 independent experiments made in triplicate. ∗Significantly different from SD rats (Student's t-test). The significance was established at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390177&req=5

fig4: Protein levels of chemokines and their receptors in the brainstem of Sprague-Dawley (SD) and taiep rats. Chemokines, receptors, and growth factors were assayed using indirect ELISA. Each value represents the mean ± SEM of 5 independent experiments made in triplicate. ∗Significantly different from SD rats (Student's t-test). The significance was established at P < 0.05.
Mentions: Comparison of protein levels between taiep rats and SD rats showed changes in chemokine receptors and growth factors at both ages. At 1 month of age, receptors that increased were CCR2 (43.5 ± 5.1%), CCR8 (111.3 ± 12.4%), CXCR4 (115.8 ± 18.1%), and FGF2 (27.6% ± 4.1). In contrast, CCR5 (−19.8 ± 5.1) and CXCL1 (−82.2 ± 1.6%) were decreased (Figure 4). In the brainstem of 6-month-old rats, protein levels were decreased for CCL2 (−39.0% ± 5.5%), CXCL1 (−80.9% ± 3.6%), CCR1 (−14.4% ± 7.9%), CCR2 (−41.2% ± 1.4%), CCR5 (−21.8% ± 5.8%), CCR8 (−34.6% ± 5.8%), CXCR4 (−54.4% ± 3.7%), and, the growth factor, FGF2 (−42.7% ± 2.7%). In contrast, VEGFA (125.5% ± 29.7%) levels increased (Figure 4).

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

Show MeSH
Related in: MedlinePlus