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Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

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Related in: MedlinePlus

Overview of scatter plot on expression of 84 genes. Analysis of gene expression in the brainstem at different ages of Sprague-Dawley and taiep rats by RT2 Profiler PCR Array. The black line indicates fold changes [2(−ΔCt)] of 1. The pink lines indicate the desired fold change in gene expression threshold, defined by the user with the entry in cell A1. SD1M = 1-month-old Sprague-Dawley (SD) rats; T1M = 1-month-old taiep rats; SD6M = 6-month-old SD rats; T6M = 6-month-old taiep rats.
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fig3: Overview of scatter plot on expression of 84 genes. Analysis of gene expression in the brainstem at different ages of Sprague-Dawley and taiep rats by RT2 Profiler PCR Array. The black line indicates fold changes [2(−ΔCt)] of 1. The pink lines indicate the desired fold change in gene expression threshold, defined by the user with the entry in cell A1. SD1M = 1-month-old Sprague-Dawley (SD) rats; T1M = 1-month-old taiep rats; SD6M = 6-month-old SD rats; T6M = 6-month-old taiep rats.

Mentions: Comparisons between results from 6-month-old versus 1-month-old rats in the same group show no significant changes in gene expression in SD rats, except for two downregulated chemokines, CXCL9 (−3.9, P < 0.05) and TGFB1 (−3.6, P < 0.05), while, in taiep rats, gene expression was downregulated for 5 chemokines: CCR8 (−5.2, P = 0.05), CCR9 (−7.7, P < 0.05), CXCL5 (−2.2, P < 0.05), MAPK1 (−15.3, P < 0.05), and TGFB1 (−3.5, P < 0.05) (Figure 3).


Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Overview of scatter plot on expression of 84 genes. Analysis of gene expression in the brainstem at different ages of Sprague-Dawley and taiep rats by RT2 Profiler PCR Array. The black line indicates fold changes [2(−ΔCt)] of 1. The pink lines indicate the desired fold change in gene expression threshold, defined by the user with the entry in cell A1. SD1M = 1-month-old Sprague-Dawley (SD) rats; T1M = 1-month-old taiep rats; SD6M = 6-month-old SD rats; T6M = 6-month-old taiep rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390177&req=5

fig3: Overview of scatter plot on expression of 84 genes. Analysis of gene expression in the brainstem at different ages of Sprague-Dawley and taiep rats by RT2 Profiler PCR Array. The black line indicates fold changes [2(−ΔCt)] of 1. The pink lines indicate the desired fold change in gene expression threshold, defined by the user with the entry in cell A1. SD1M = 1-month-old Sprague-Dawley (SD) rats; T1M = 1-month-old taiep rats; SD6M = 6-month-old SD rats; T6M = 6-month-old taiep rats.
Mentions: Comparisons between results from 6-month-old versus 1-month-old rats in the same group show no significant changes in gene expression in SD rats, except for two downregulated chemokines, CXCL9 (−3.9, P < 0.05) and TGFB1 (−3.6, P < 0.05), while, in taiep rats, gene expression was downregulated for 5 chemokines: CCR8 (−5.2, P = 0.05), CCR9 (−7.7, P < 0.05), CXCL5 (−2.2, P < 0.05), MAPK1 (−15.3, P < 0.05), and TGFB1 (−3.5, P < 0.05) (Figure 3).

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

Show MeSH
Related in: MedlinePlus