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Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

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Related in: MedlinePlus

Chemokine expression profile in the brainstem of taiep rats 1 and 6 months old. Deregulation in chemokines and chemokine receptors was determined by RT2 Profiler PCR Array in the brainstem of taiep rats one and six months old. Values show fold up- or downregulation as compared to control group, Sprague-Dawley rats at the same age.
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fig2: Chemokine expression profile in the brainstem of taiep rats 1 and 6 months old. Deregulation in chemokines and chemokine receptors was determined by RT2 Profiler PCR Array in the brainstem of taiep rats one and six months old. Values show fold up- or downregulation as compared to control group, Sprague-Dawley rats at the same age.

Mentions: The scatter plots show the expression profile of 84 genes of taiep rat in comparison with the control profile of SD rats (Figure 1). No upregulation of proinflammatory cytokines was determined at any age and group studied. In contrast, downregulation was established for IL-1β (−3.3, P < 0.05) and IL-10 (−8.0, P < 0.05) at 1-month-old, ITG-β2 at both 1- (−59.3, P = 0.01) and 6-month-old (−5.4, P = 0.01), and MAPK1 at 6-month-old (−29.9, P = 0.01) taiep rats (Figure 2). Downregulation was also established for inflammation mediators such as formyl peptide receptor 1 (FPR1) at 1- (−13.5, P < 0.05) and at 6-month-old (−5.31, P < 0.05) and GPR17 at 1- (−7.03, P = 0.05) and at 6-month-old (−4.6, P < 0.05) taiep rats. These results show that taiep rat brainstem exhibits a decrease in inflammatory and anti-inflammatory cytokine.


Analysis of chemokines and receptors expression profile in the myelin mutant taiep rat.

Soto-Rodriguez G, Gonzalez-Barrios JA, Martinez-Fong D, Blanco-Alvarez VM, Eguibar JR, Ugarte A, Martinez-Perez F, Brambila E, Peña LM, Pazos-Salazar NG, Torres-Soto M, Garcia-Robles G, Tomas-Sanchez C, Leon-Chavez BA - Oxid Med Cell Longev (2015)

Chemokine expression profile in the brainstem of taiep rats 1 and 6 months old. Deregulation in chemokines and chemokine receptors was determined by RT2 Profiler PCR Array in the brainstem of taiep rats one and six months old. Values show fold up- or downregulation as compared to control group, Sprague-Dawley rats at the same age.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390177&req=5

fig2: Chemokine expression profile in the brainstem of taiep rats 1 and 6 months old. Deregulation in chemokines and chemokine receptors was determined by RT2 Profiler PCR Array in the brainstem of taiep rats one and six months old. Values show fold up- or downregulation as compared to control group, Sprague-Dawley rats at the same age.
Mentions: The scatter plots show the expression profile of 84 genes of taiep rat in comparison with the control profile of SD rats (Figure 1). No upregulation of proinflammatory cytokines was determined at any age and group studied. In contrast, downregulation was established for IL-1β (−3.3, P < 0.05) and IL-10 (−8.0, P < 0.05) at 1-month-old, ITG-β2 at both 1- (−59.3, P = 0.01) and 6-month-old (−5.4, P = 0.01), and MAPK1 at 6-month-old (−29.9, P = 0.01) taiep rats (Figure 2). Downregulation was also established for inflammation mediators such as formyl peptide receptor 1 (FPR1) at 1- (−13.5, P < 0.05) and at 6-month-old (−5.31, P < 0.05) and GPR17 at 1- (−7.03, P = 0.05) and at 6-month-old (−4.6, P < 0.05) taiep rats. These results show that taiep rat brainstem exhibits a decrease in inflammatory and anti-inflammatory cytokine.

Bottom Line: PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat.ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months.These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

View Article: PubMed Central - PubMed

Affiliation: Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, 14 Sur y Avenida San Claudio, 72570 Puebla, PUE, Mexico.

ABSTRACT
Taiep rat has a failure in myelination and remyelination processes leading to a state of hypomyelination throughout its life. Chemokines, which are known to play a role in inflammation, are also involved in the remyelination process. We aimed to demonstrate that remyelination-stimulating factors are altered in the brainstem of 1- and 6-month-old taiep rats. We used a Rat RT(2) Profiler PCR Array to assess mRNA expression of 84 genes coding for cytokines, chemokines, and their receptors. We also evaluated protein levels of CCL2, CCR1, CCR2, CCL5, CCR5, CCR8, CXCL1, CXCR2, CXCR4, FGF2, and VEGFA by ELISA. Sprague-Dawley rats were used as a control. PCR Array procedure showed that proinflammatory cytokines were not upregulated in the taiep rat. In contrast, some mRNA levels of beta and alpha chemokines were upregulated in 1-month-old rats, but CXCR4 was downregulated at their 6 months of age. ELISA results showed that CXCL1, CCL2, CCR2, CCR5, CCR8, and CXCR4 protein levels were decreased in brainstem at the age of 6 months. These results suggest the presence of a chronic neuroinflammation process with deficiency of remyelination-stimulating factors (CXCL1, CXCR2, and CXCR4), which might account for the demyelination in the taiep rat.

Show MeSH
Related in: MedlinePlus