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Recent trends in rapid environmental monitoring of pathogens and toxicants: potential of nanoparticle-based biosensor and applications.

Koedrith P, Thasiphu T, Weon JI, Boonprasert R, Tuitemwong K, Tuitemwong P - ScientificWorldJournal (2015)

Bottom Line: The increase of cells to detection levels requires long incubation time.The major approach is to use nanoparticles as signal reporter to increase output rather than spending time to increase cell concentrations.Trends in future development of novel detection devices and their advantages over other environmental monitoring methodologies are also discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environment and Resource Studies, Mahidol University, Phutthamonthon District, Nakhon Pathom 73170, Thailand.

ABSTRACT
Of global concern, environmental pollution adversely affects human health and socioeconomic development. The presence of environmental contaminants, especially bacterial, viral, and parasitic pathogens and their toxins as well as chemical substances, poses serious public health concerns. Nanoparticle-based biosensors are considered as potential tools for rapid, specific, and highly sensitive detection of the analyte of interest (both biotic and abiotic contaminants). In particular, there are several limitations of conventional detection methods for water-borne pathogens due to low concentrations and interference with various enzymatic inhibitors in the environmental samples. The increase of cells to detection levels requires long incubation time. This review describes current state of biosensor nanotechnology, the advantage over conventional detection methods, and the challenges due to testing of environmental samples. The major approach is to use nanoparticles as signal reporter to increase output rather than spending time to increase cell concentrations. Trends in future development of novel detection devices and their advantages over other environmental monitoring methodologies are also discussed.

No MeSH data available.


Related in: MedlinePlus

Common procedures for detection of certain water-borne pathogens in environmental matrices and progressive development of respective bionanosensors including immunosensor, DNA-based sensor, and others. Irrespectively, preprocessing steps of necessity initially require filtration and concentration, and then an immunoseparation step (e.g., immunomagnetic separation) in several types of assays.
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Related In: Results  -  Collection


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fig4: Common procedures for detection of certain water-borne pathogens in environmental matrices and progressive development of respective bionanosensors including immunosensor, DNA-based sensor, and others. Irrespectively, preprocessing steps of necessity initially require filtration and concentration, and then an immunoseparation step (e.g., immunomagnetic separation) in several types of assays.

Mentions: Recently, nucleic acid biosensor-based researches have been increasingly focused. Nucleic acid biosensors offer desirable sensitivity for detecting particularly water-borne pathogens even at low levels (Figure 4) [57, 58]. At diverging point, immunosensors (left panels) require only filtration, concentration, and detection while DNA biosensors (central panels) possibly need consecutive preprocessing of cellular component disruption, genetic material purification, and often enzymatic amplification and/or hybridization. Aforementioned sensors can be established in either label-based or label-free detection system. Typical detection methods are exemplified in column boxes.


Recent trends in rapid environmental monitoring of pathogens and toxicants: potential of nanoparticle-based biosensor and applications.

Koedrith P, Thasiphu T, Weon JI, Boonprasert R, Tuitemwong K, Tuitemwong P - ScientificWorldJournal (2015)

Common procedures for detection of certain water-borne pathogens in environmental matrices and progressive development of respective bionanosensors including immunosensor, DNA-based sensor, and others. Irrespectively, preprocessing steps of necessity initially require filtration and concentration, and then an immunoseparation step (e.g., immunomagnetic separation) in several types of assays.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390168&req=5

fig4: Common procedures for detection of certain water-borne pathogens in environmental matrices and progressive development of respective bionanosensors including immunosensor, DNA-based sensor, and others. Irrespectively, preprocessing steps of necessity initially require filtration and concentration, and then an immunoseparation step (e.g., immunomagnetic separation) in several types of assays.
Mentions: Recently, nucleic acid biosensor-based researches have been increasingly focused. Nucleic acid biosensors offer desirable sensitivity for detecting particularly water-borne pathogens even at low levels (Figure 4) [57, 58]. At diverging point, immunosensors (left panels) require only filtration, concentration, and detection while DNA biosensors (central panels) possibly need consecutive preprocessing of cellular component disruption, genetic material purification, and often enzymatic amplification and/or hybridization. Aforementioned sensors can be established in either label-based or label-free detection system. Typical detection methods are exemplified in column boxes.

Bottom Line: The increase of cells to detection levels requires long incubation time.The major approach is to use nanoparticles as signal reporter to increase output rather than spending time to increase cell concentrations.Trends in future development of novel detection devices and their advantages over other environmental monitoring methodologies are also discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environment and Resource Studies, Mahidol University, Phutthamonthon District, Nakhon Pathom 73170, Thailand.

ABSTRACT
Of global concern, environmental pollution adversely affects human health and socioeconomic development. The presence of environmental contaminants, especially bacterial, viral, and parasitic pathogens and their toxins as well as chemical substances, poses serious public health concerns. Nanoparticle-based biosensors are considered as potential tools for rapid, specific, and highly sensitive detection of the analyte of interest (both biotic and abiotic contaminants). In particular, there are several limitations of conventional detection methods for water-borne pathogens due to low concentrations and interference with various enzymatic inhibitors in the environmental samples. The increase of cells to detection levels requires long incubation time. This review describes current state of biosensor nanotechnology, the advantage over conventional detection methods, and the challenges due to testing of environmental samples. The major approach is to use nanoparticles as signal reporter to increase output rather than spending time to increase cell concentrations. Trends in future development of novel detection devices and their advantages over other environmental monitoring methodologies are also discussed.

No MeSH data available.


Related in: MedlinePlus