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Effects of maternal diet and exercise during pregnancy on glucose metabolism in skeletal muscle and fat of weanling rats.

Raipuria M, Bahari H, Morris MJ - PLoS ONE (2015)

Bottom Line: At PND1, both male and female pups from exercised lean dams were significantly lighter (CE versus CS), with no effect in those from obese dams.Exercise significantly reduced insulin concentrations in males (CE/FE versus CS/FS), with reduced glucose in male FE pups.Maternal exercise appeared to decrease the metabolic risk induced by maternal obesity, improving insulin/glucose metabolism, with greater effects in male than female offspring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medical Sciences, UNSW Australia, Sydney, NSW 2052, Australia.

ABSTRACT
Obesity during pregnancy contributes to the development of metabolic disorders in offspring. Maternal exercise may limit gestational weight gain and ameliorate these programming effects. We previously showed benefits of post-weaning voluntary exercise in offspring from obese dams. Here we examined whether voluntary exercise during pregnancy influences lipid and glucose homeostasis in muscle and fat in offspring of both lean and obese dams. Female Sprague-Dawley rats were fed chow (C) or high fat (F) diet for 6 weeks before mating. Half underwent voluntary exercise (CE/FE) with a running wheel introduced 10 days prior to mating and available until the dams delivered; others remained sedentary (CS/FS). Male and female pups were killed at postnatal day (PND)19 and retroperitoneal fat and gastrocnemius muscle were collected for gene expression. Lean and obese dams achieved similar modest levels of exercise. At PND1, both male and female pups from exercised lean dams were significantly lighter (CE versus CS), with no effect in those from obese dams. At PND19, maternal obesity significantly increased offspring body weight and adiposity, with no effect of maternal exercise. Exercise significantly reduced insulin concentrations in males (CE/FE versus CS/FS), with reduced glucose in male FE pups. In males, maternal obesity significantly decreased muscle myogenic differentiation 1 (MYOD1) and glucose transporter type 4 (GLUT4) mRNA expressions (FS vs CS); these were normalized by exercise. Maternal exercise upregulated adipose GLUT4, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and peroxisome proliferator activated receptor gamma coactivator 1 alpha (PGC1α) mRNA expression in offspring of dams consuming chow. Modest voluntary exercise during pregnancy was associated with lower birth weight in pups from lean dams. Maternal exercise appeared to decrease the metabolic risk induced by maternal obesity, improving insulin/glucose metabolism, with greater effects in male than female offspring.

No MeSH data available.


Related in: MedlinePlus

Maternal blood glucose concentration during the lactation period, at postpartum day 12.Data are expressed as mean ± SEM. The first letter describes maternal diet; chow (C) or HFD (F) and the second letter describes maternal activity; sedentary (S) or exercise (E). Data were analyzed by two-way ANOVA followed by post hoc LSD. **P< .01 maternal HFD effect; ††P < .01 maternal exercise effect.
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pone.0120980.g001: Maternal blood glucose concentration during the lactation period, at postpartum day 12.Data are expressed as mean ± SEM. The first letter describes maternal diet; chow (C) or HFD (F) and the second letter describes maternal activity; sedentary (S) or exercise (E). Data were analyzed by two-way ANOVA followed by post hoc LSD. **P< .01 maternal HFD effect; ††P < .01 maternal exercise effect.

Mentions: Maternal exercise during pregnancy had beneficial effects in obese dams by maintaining blood glucose at similar levels to control dams during the lactation period (postpartum day 12, Fig. 1). There was no significant difference in blood glucose level at 4 weeks postpartum across groups (Table 1). Plasma insulin concentrations were significantly increased by HFD consumption in obese dams regardless of their exercise activity (P < .01, Table 1). No effect of exercise on plasma insulin was observed. Maternal HFD consumption was associated with hyperlipidemia; plasma triglyceride levels were significantly increased in sedentary obese dams with a reduction in obese exercising dams that did not reach significance (FE vs FS, P < .06, Table 1).


Effects of maternal diet and exercise during pregnancy on glucose metabolism in skeletal muscle and fat of weanling rats.

Raipuria M, Bahari H, Morris MJ - PLoS ONE (2015)

Maternal blood glucose concentration during the lactation period, at postpartum day 12.Data are expressed as mean ± SEM. The first letter describes maternal diet; chow (C) or HFD (F) and the second letter describes maternal activity; sedentary (S) or exercise (E). Data were analyzed by two-way ANOVA followed by post hoc LSD. **P< .01 maternal HFD effect; ††P < .01 maternal exercise effect.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390148&req=5

pone.0120980.g001: Maternal blood glucose concentration during the lactation period, at postpartum day 12.Data are expressed as mean ± SEM. The first letter describes maternal diet; chow (C) or HFD (F) and the second letter describes maternal activity; sedentary (S) or exercise (E). Data were analyzed by two-way ANOVA followed by post hoc LSD. **P< .01 maternal HFD effect; ††P < .01 maternal exercise effect.
Mentions: Maternal exercise during pregnancy had beneficial effects in obese dams by maintaining blood glucose at similar levels to control dams during the lactation period (postpartum day 12, Fig. 1). There was no significant difference in blood glucose level at 4 weeks postpartum across groups (Table 1). Plasma insulin concentrations were significantly increased by HFD consumption in obese dams regardless of their exercise activity (P < .01, Table 1). No effect of exercise on plasma insulin was observed. Maternal HFD consumption was associated with hyperlipidemia; plasma triglyceride levels were significantly increased in sedentary obese dams with a reduction in obese exercising dams that did not reach significance (FE vs FS, P < .06, Table 1).

Bottom Line: At PND1, both male and female pups from exercised lean dams were significantly lighter (CE versus CS), with no effect in those from obese dams.Exercise significantly reduced insulin concentrations in males (CE/FE versus CS/FS), with reduced glucose in male FE pups.Maternal exercise appeared to decrease the metabolic risk induced by maternal obesity, improving insulin/glucose metabolism, with greater effects in male than female offspring.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medical Sciences, UNSW Australia, Sydney, NSW 2052, Australia.

ABSTRACT
Obesity during pregnancy contributes to the development of metabolic disorders in offspring. Maternal exercise may limit gestational weight gain and ameliorate these programming effects. We previously showed benefits of post-weaning voluntary exercise in offspring from obese dams. Here we examined whether voluntary exercise during pregnancy influences lipid and glucose homeostasis in muscle and fat in offspring of both lean and obese dams. Female Sprague-Dawley rats were fed chow (C) or high fat (F) diet for 6 weeks before mating. Half underwent voluntary exercise (CE/FE) with a running wheel introduced 10 days prior to mating and available until the dams delivered; others remained sedentary (CS/FS). Male and female pups were killed at postnatal day (PND)19 and retroperitoneal fat and gastrocnemius muscle were collected for gene expression. Lean and obese dams achieved similar modest levels of exercise. At PND1, both male and female pups from exercised lean dams were significantly lighter (CE versus CS), with no effect in those from obese dams. At PND19, maternal obesity significantly increased offspring body weight and adiposity, with no effect of maternal exercise. Exercise significantly reduced insulin concentrations in males (CE/FE versus CS/FS), with reduced glucose in male FE pups. In males, maternal obesity significantly decreased muscle myogenic differentiation 1 (MYOD1) and glucose transporter type 4 (GLUT4) mRNA expressions (FS vs CS); these were normalized by exercise. Maternal exercise upregulated adipose GLUT4, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and peroxisome proliferator activated receptor gamma coactivator 1 alpha (PGC1α) mRNA expression in offspring of dams consuming chow. Modest voluntary exercise during pregnancy was associated with lower birth weight in pups from lean dams. Maternal exercise appeared to decrease the metabolic risk induced by maternal obesity, improving insulin/glucose metabolism, with greater effects in male than female offspring.

No MeSH data available.


Related in: MedlinePlus