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The Neuroprotective Mechanism of Low-Frequency rTMS on Nigral Dopaminergic Neurons of Parkinson's Disease Model Mice.

Dong Q, Wang Y, Gu P, Shao R, Zhao L, Liu X, Wang Z, Wang M - Parkinsons Dis (2015)

Bottom Line: Results.Conclusions.Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor.

View Article: PubMed Central - PubMed

Affiliation: Fifth Department of Neurology, Cangzhou Central Hospital, No. 16 Xinhua Western Road, Cangzhou, Hebei 061000, China.

ABSTRACT
Background. Parkinson's disease is a neurodegenerative disease in elder people, pathophysiologic basis of which is the severe deficiency of dopamine in the striatum. The purpose of the present study was to evaluate the neuroprotective effect of low-frequency rTMS on Parkinson's disease in model mice. Methods. The effects of low-frequency rTMS on the motor function, cortex excitability, neurochemistry, and neurohistopathology of MPTP-induced Parkinson's disease mice were investigated through behavioral detection, electrophysiologic technique, high performance liquid chromatography-electrochemical detection, immunohistochemical staining, and western blot. Results. Low-frequency rTMS could improve the motor coordination impairment of Parkinson's disease mice: the resting motor threshold significantly decreased in the Parkinson's disease mice; the degeneration of nigral dopaminergic neuron and the expression of tyrosine hydroxylase were significantly improved by low-frequency rTMS; moreover, the expressions of brain derived neurotrophic factor and glial cell line derived neurotrophic factor were also improved by low-frequency rTMS. Conclusions. Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor. The present study provided a theoretical basis for the application of low-frequency rTMS in the clinical treatment and recovery of Parkinson's disease.

No MeSH data available.


Related in: MedlinePlus

The effect of low-frequency rTMS on the expression of TH, BDNF, and GDNF as illustrated by western blot. *Significantly different from NS group, P < 0.05; #significantly different from rTMS group, P < 0.05.
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fig6: The effect of low-frequency rTMS on the expression of TH, BDNF, and GDNF as illustrated by western blot. *Significantly different from NS group, P < 0.05; #significantly different from rTMS group, P < 0.05.

Mentions: The results of immunohistochemical staining were shown in Figure 5 and Table S5. Generally, the expressions of TH, BDNF (including two bands: monomer and homodimer), and GDNF all significantly declined in PD model mice. However, the symptoms were reversed after treatment of low-frequency rTMS. And the results showed that the changes of COD of TH were positively related to those of BDNF and GDNF (Table 1). Same patterns were also observed in the western blot of TH, BDNF, and GDNF, which confirmed the protective effect of low-frequency rTMS on the NDN of PD model mice (Figure 6; Tables 2 and S6).


The Neuroprotective Mechanism of Low-Frequency rTMS on Nigral Dopaminergic Neurons of Parkinson's Disease Model Mice.

Dong Q, Wang Y, Gu P, Shao R, Zhao L, Liu X, Wang Z, Wang M - Parkinsons Dis (2015)

The effect of low-frequency rTMS on the expression of TH, BDNF, and GDNF as illustrated by western blot. *Significantly different from NS group, P < 0.05; #significantly different from rTMS group, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390107&req=5

fig6: The effect of low-frequency rTMS on the expression of TH, BDNF, and GDNF as illustrated by western blot. *Significantly different from NS group, P < 0.05; #significantly different from rTMS group, P < 0.05.
Mentions: The results of immunohistochemical staining were shown in Figure 5 and Table S5. Generally, the expressions of TH, BDNF (including two bands: monomer and homodimer), and GDNF all significantly declined in PD model mice. However, the symptoms were reversed after treatment of low-frequency rTMS. And the results showed that the changes of COD of TH were positively related to those of BDNF and GDNF (Table 1). Same patterns were also observed in the western blot of TH, BDNF, and GDNF, which confirmed the protective effect of low-frequency rTMS on the NDN of PD model mice (Figure 6; Tables 2 and S6).

Bottom Line: Results.Conclusions.Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor.

View Article: PubMed Central - PubMed

Affiliation: Fifth Department of Neurology, Cangzhou Central Hospital, No. 16 Xinhua Western Road, Cangzhou, Hebei 061000, China.

ABSTRACT
Background. Parkinson's disease is a neurodegenerative disease in elder people, pathophysiologic basis of which is the severe deficiency of dopamine in the striatum. The purpose of the present study was to evaluate the neuroprotective effect of low-frequency rTMS on Parkinson's disease in model mice. Methods. The effects of low-frequency rTMS on the motor function, cortex excitability, neurochemistry, and neurohistopathology of MPTP-induced Parkinson's disease mice were investigated through behavioral detection, electrophysiologic technique, high performance liquid chromatography-electrochemical detection, immunohistochemical staining, and western blot. Results. Low-frequency rTMS could improve the motor coordination impairment of Parkinson's disease mice: the resting motor threshold significantly decreased in the Parkinson's disease mice; the degeneration of nigral dopaminergic neuron and the expression of tyrosine hydroxylase were significantly improved by low-frequency rTMS; moreover, the expressions of brain derived neurotrophic factor and glial cell line derived neurotrophic factor were also improved by low-frequency rTMS. Conclusions. Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor. The present study provided a theoretical basis for the application of low-frequency rTMS in the clinical treatment and recovery of Parkinson's disease.

No MeSH data available.


Related in: MedlinePlus