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The Neuroprotective Mechanism of Low-Frequency rTMS on Nigral Dopaminergic Neurons of Parkinson's Disease Model Mice.

Dong Q, Wang Y, Gu P, Shao R, Zhao L, Liu X, Wang Z, Wang M - Parkinsons Dis (2015)

Bottom Line: Results.Conclusions.Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor.

View Article: PubMed Central - PubMed

Affiliation: Fifth Department of Neurology, Cangzhou Central Hospital, No. 16 Xinhua Western Road, Cangzhou, Hebei 061000, China.

ABSTRACT
Background. Parkinson's disease is a neurodegenerative disease in elder people, pathophysiologic basis of which is the severe deficiency of dopamine in the striatum. The purpose of the present study was to evaluate the neuroprotective effect of low-frequency rTMS on Parkinson's disease in model mice. Methods. The effects of low-frequency rTMS on the motor function, cortex excitability, neurochemistry, and neurohistopathology of MPTP-induced Parkinson's disease mice were investigated through behavioral detection, electrophysiologic technique, high performance liquid chromatography-electrochemical detection, immunohistochemical staining, and western blot. Results. Low-frequency rTMS could improve the motor coordination impairment of Parkinson's disease mice: the resting motor threshold significantly decreased in the Parkinson's disease mice; the degeneration of nigral dopaminergic neuron and the expression of tyrosine hydroxylase were significantly improved by low-frequency rTMS; moreover, the expressions of brain derived neurotrophic factor and glial cell line derived neurotrophic factor were also improved by low-frequency rTMS. Conclusions. Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor. The present study provided a theoretical basis for the application of low-frequency rTMS in the clinical treatment and recovery of Parkinson's disease.

No MeSH data available.


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The comparison of the locomotor activity in different groups at different time points.
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fig2: The comparison of the locomotor activity in different groups at different time points.

Mentions: Shortly after the first injection of MPTP, model mice showed performance of piloerection, bradykinesia, stroub tail reaction, instability of gait, tremor, and the toes of the hind feet widely separated. Those performances remained in MPTP-treated mice for 5 to 6 h. Similar symptoms were observed after the subsequent injections, and the symptoms of bradykinesia, stiffness, and instability of gait strengthened with the injection of MPTP. However, all the mice showed recovery as regards external appearance 24 h after injection. No abnormality was observed in the control group. No dysfunction was observed in the stimulated animals during or after low-frequency rTMS. The results of automated locomotor activity test showed that treatment of low-frequency rTMS had no improvement on the locomotor activity of model mice (Figure 1; See Supplementary Table S1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/564095); however, significant effect of low-frequency rTMS on the improvement of rotary number was observed in rotary test (Figure 2; Table S2).


The Neuroprotective Mechanism of Low-Frequency rTMS on Nigral Dopaminergic Neurons of Parkinson's Disease Model Mice.

Dong Q, Wang Y, Gu P, Shao R, Zhao L, Liu X, Wang Z, Wang M - Parkinsons Dis (2015)

The comparison of the locomotor activity in different groups at different time points.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390107&req=5

fig2: The comparison of the locomotor activity in different groups at different time points.
Mentions: Shortly after the first injection of MPTP, model mice showed performance of piloerection, bradykinesia, stroub tail reaction, instability of gait, tremor, and the toes of the hind feet widely separated. Those performances remained in MPTP-treated mice for 5 to 6 h. Similar symptoms were observed after the subsequent injections, and the symptoms of bradykinesia, stiffness, and instability of gait strengthened with the injection of MPTP. However, all the mice showed recovery as regards external appearance 24 h after injection. No abnormality was observed in the control group. No dysfunction was observed in the stimulated animals during or after low-frequency rTMS. The results of automated locomotor activity test showed that treatment of low-frequency rTMS had no improvement on the locomotor activity of model mice (Figure 1; See Supplementary Table S1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/564095); however, significant effect of low-frequency rTMS on the improvement of rotary number was observed in rotary test (Figure 2; Table S2).

Bottom Line: Results.Conclusions.Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor.

View Article: PubMed Central - PubMed

Affiliation: Fifth Department of Neurology, Cangzhou Central Hospital, No. 16 Xinhua Western Road, Cangzhou, Hebei 061000, China.

ABSTRACT
Background. Parkinson's disease is a neurodegenerative disease in elder people, pathophysiologic basis of which is the severe deficiency of dopamine in the striatum. The purpose of the present study was to evaluate the neuroprotective effect of low-frequency rTMS on Parkinson's disease in model mice. Methods. The effects of low-frequency rTMS on the motor function, cortex excitability, neurochemistry, and neurohistopathology of MPTP-induced Parkinson's disease mice were investigated through behavioral detection, electrophysiologic technique, high performance liquid chromatography-electrochemical detection, immunohistochemical staining, and western blot. Results. Low-frequency rTMS could improve the motor coordination impairment of Parkinson's disease mice: the resting motor threshold significantly decreased in the Parkinson's disease mice; the degeneration of nigral dopaminergic neuron and the expression of tyrosine hydroxylase were significantly improved by low-frequency rTMS; moreover, the expressions of brain derived neurotrophic factor and glial cell line derived neurotrophic factor were also improved by low-frequency rTMS. Conclusions. Low-frequency rTMS had a neuroprotective effect on the nigral dopaminergic neuron which might be due to the improved expressions of brain derived neurotrophic factor and glial cell line-derived neurotrophic factor. The present study provided a theoretical basis for the application of low-frequency rTMS in the clinical treatment and recovery of Parkinson's disease.

No MeSH data available.


Related in: MedlinePlus