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Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

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Related in: MedlinePlus

Representative microscopic images showing the blood vessels immunostained by vWF in the explanted scaffolds after subcutaneous implantation for 2 (a–d) and 4 (e–h) weeks (n = 3): PCL (a and e); VEGF loaded PCL (b and f); VEGF loaded Hep-PCL/Gel-1 (c and g); VEGF loaded Hep-PCL/Gel-2 (d and h) (scale bars = 50μm). Quantitative analysis on the density of blood vessel (i). *P < 0.05, #P < 0.001.
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fig7: Representative microscopic images showing the blood vessels immunostained by vWF in the explanted scaffolds after subcutaneous implantation for 2 (a–d) and 4 (e–h) weeks (n = 3): PCL (a and e); VEGF loaded PCL (b and f); VEGF loaded Hep-PCL/Gel-1 (c and g); VEGF loaded Hep-PCL/Gel-2 (d and h) (scale bars = 50μm). Quantitative analysis on the density of blood vessel (i). *P < 0.05, #P < 0.001.

Mentions: The blood vessels in the cellularized area were analyzed by immunofluorescent staining with vWF (Figures 7(a)–7(h)) and further quantified based on the image. More capillaries could be clearly identified on the VEGF loaded PCL/Gel scaffolds (arrow indicated). The quantitative result further demonstrates that VEGF loaded PCL/Gel scaffolds significantly enhanced the vessel density compared to PCL and VEGF loaded PCL (P < 0.001) after 4 weeks. This enhancement is closely associated with heparin for VEGF loading and protecting, which agrees well with in vitro cell proliferation (Figure 5).


Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

Representative microscopic images showing the blood vessels immunostained by vWF in the explanted scaffolds after subcutaneous implantation for 2 (a–d) and 4 (e–h) weeks (n = 3): PCL (a and e); VEGF loaded PCL (b and f); VEGF loaded Hep-PCL/Gel-1 (c and g); VEGF loaded Hep-PCL/Gel-2 (d and h) (scale bars = 50μm). Quantitative analysis on the density of blood vessel (i). *P < 0.05, #P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390103&req=5

fig7: Representative microscopic images showing the blood vessels immunostained by vWF in the explanted scaffolds after subcutaneous implantation for 2 (a–d) and 4 (e–h) weeks (n = 3): PCL (a and e); VEGF loaded PCL (b and f); VEGF loaded Hep-PCL/Gel-1 (c and g); VEGF loaded Hep-PCL/Gel-2 (d and h) (scale bars = 50μm). Quantitative analysis on the density of blood vessel (i). *P < 0.05, #P < 0.001.
Mentions: The blood vessels in the cellularized area were analyzed by immunofluorescent staining with vWF (Figures 7(a)–7(h)) and further quantified based on the image. More capillaries could be clearly identified on the VEGF loaded PCL/Gel scaffolds (arrow indicated). The quantitative result further demonstrates that VEGF loaded PCL/Gel scaffolds significantly enhanced the vessel density compared to PCL and VEGF loaded PCL (P < 0.001) after 4 weeks. This enhancement is closely associated with heparin for VEGF loading and protecting, which agrees well with in vitro cell proliferation (Figure 5).

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

Show MeSH
Related in: MedlinePlus