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Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

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Related in: MedlinePlus

MTT assay for cell proliferation of human umbilical vein endothelial cells (HUVECs) on the VEGF loaded PCL and heparinized PCL/Gel scaffolds. Neat PCL and tissue culture plate (TCP) were used as control (n = 5). *P < 0.05; #P < 0.001.
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fig5: MTT assay for cell proliferation of human umbilical vein endothelial cells (HUVECs) on the VEGF loaded PCL and heparinized PCL/Gel scaffolds. Neat PCL and tissue culture plate (TCP) were used as control (n = 5). *P < 0.05; #P < 0.001.

Mentions: The effect of sustained release of VEGF on the endothelial cell proliferation was investigated by in vitro MTT assay. As shown in Figure 5, the cell proliferation was significantly (P < 0.05) accelerated on VEGF loaded Hep-PCL/Gel scaffolds compared with that on the neat PCL throughout the entire culture process. In addition, cells grew faster on the VEGF loaded Hep-PCL/Gel-2 than on the VEGF loaded Hep-PCL/Gel-1 scaffold, which may be due to the higher VEGF loading. VEGF loaded on the PCL scaffold slightly increases the cell proliferation, but the effect is not pronounced. The enhanced proliferation on the VEGF loaded Hep-PCL/Gel scaffolds also confirms that the bioactivity of VEGF released from the scaffolds was higher than PCL scaffolds due to the protective effect provided by immobilized heparin.


Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

MTT assay for cell proliferation of human umbilical vein endothelial cells (HUVECs) on the VEGF loaded PCL and heparinized PCL/Gel scaffolds. Neat PCL and tissue culture plate (TCP) were used as control (n = 5). *P < 0.05; #P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390103&req=5

fig5: MTT assay for cell proliferation of human umbilical vein endothelial cells (HUVECs) on the VEGF loaded PCL and heparinized PCL/Gel scaffolds. Neat PCL and tissue culture plate (TCP) were used as control (n = 5). *P < 0.05; #P < 0.001.
Mentions: The effect of sustained release of VEGF on the endothelial cell proliferation was investigated by in vitro MTT assay. As shown in Figure 5, the cell proliferation was significantly (P < 0.05) accelerated on VEGF loaded Hep-PCL/Gel scaffolds compared with that on the neat PCL throughout the entire culture process. In addition, cells grew faster on the VEGF loaded Hep-PCL/Gel-2 than on the VEGF loaded Hep-PCL/Gel-1 scaffold, which may be due to the higher VEGF loading. VEGF loaded on the PCL scaffold slightly increases the cell proliferation, but the effect is not pronounced. The enhanced proliferation on the VEGF loaded Hep-PCL/Gel scaffolds also confirms that the bioactivity of VEGF released from the scaffolds was higher than PCL scaffolds due to the protective effect provided by immobilized heparin.

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

Show MeSH
Related in: MedlinePlus